期刊
CHEMISTRY AND PHYSICS OF LIPIDS
卷 163, 期 6, 页码 538-544出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.chemphyslip.2010.04.002
关键词
Cytochrome c; Phospholipid; Reactive oxygen species; Peroxidation; Mass spectrometry
The oxidative interaction of cytochrome c (Cyt c) with liposomes of Palmitoyl Linoleyl Phosphatidyl Choline (PLPC) initiated by radio-induced free radicals was investigated. Results showed that the peroxidation of PLPC is decreased in the presence of Cyt c, meaning that this latter is the preferential target of hydroxyl radicals. In addition, when Cyt c was incubated with peroxidized PLPC, it was found to be able to decompose hydroperoxides of PLPC into hydroxides. The peroxidase activity of Cyt c proceeded via the opening of the tertiary structure of Cyt c. as suggested by the loss of the sixth coordination bond of the heme-iron. Even if it is known to preferentially interact with cardiolipin, this work shows that Cyt c is also able to interact with hydroperoxide species of non-anionic phospholipids. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
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