期刊
CHEMISTRY AND PHYSICS OF LIPIDS
卷 159, 期 1, 页码 13-20出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.chemphyslip.2009.02.004
关键词
Phosphatidic acid; Phosphaplipase D; LC-MS; Apoptosis; Signaling development; Taxus cuspidata cells
资金
- National Science Fund of China for Distinguished Young Scholars [20425620]
- Key Program [20736006]
- National Basic Research Program 973 of China [2007CB714301]
- National Science Fund of China for Doctor [20060056022]
Degradation of membrane phospholipids is associated with apoptotic responses, but the signaling development of this degradation is not well understood. Cerium (Ce4+), an important rare earth element, induces cellular apoptosis and taxol biosynthesis in Taxus cuspidata suspension cultures. Here, using mass spectrometry and biochemical technique, we demonstrated that the phospholipase D (PLD) was rapidly activated by Ce4+ and hydrolyzed structural phospholipids to generate lipid signal molecule, phosphatidic acid (PA). I-Butanol, an antagonist of PLD-dependent PA production, blocked the biphasic burst of superoxide anions (O-2(center dot-)) and thus mitigated cellular apoptosis. The time-course analysis of PA accumulation and ERK-like mitogen-activated protein kinase (MAPK) regulation indicated PA generation preceded MAPK activation, suggesting that the rapid accumulation of PA might be required for the initial MAPK activity. After 2 h of Ce4+ elicitation, however, PA-induced O-2(center dot-) burst, forming a negative regulation to MAPK activity, which in turn led to apoptotic signaling development. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
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