Article
Neurosciences
Emil Jakobsen, Jens V. Andersen, Sofie K. Christensen, Olga Siamka, Martin R. Larsen, Helle S. Waagepetersen, Blanca I. Aldana, Lasse K. Bak
Summary: Pharmacologically induced inhibition of sAC activity lowers mitochondrial respiration, induces phosphorylation of AMP-activated protein kinase (AMPK), and decreases glycogen stores in cultured primary murine astrocytes. Mitochondrial sAC emerges as a key regulator of astrocyte bioenergetics, despite challenges in investigating the roles of cytosolic versus mitochondrial sAC in astrocytes using current pharmacological tools.
Article
Biochemistry & Molecular Biology
Baojun Sun, Hitomi Maruta, Yun Ma, Hiromi Yamashita
Summary: Taurine is an abundant free amino acid in mammalian tissues that plays a role in skeletal muscle function and exercise capacity. This study investigated the mechanism of taurine function in skeletal muscles by examining the effects of short-term administration of taurine on rats and L6 cells. The results suggest that taurine modulates skeletal muscle function by activating AMP-activated protein kinase through the calcium signaling pathway, leading to the expression of genes and proteins associated with mitochondrial and respiratory metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Fuli Shi, Zhimin Tang, Shanshan Jiang, Zhijuan Xiong, Wansi Zhang, Yuanjun Li, Hui Lin, Zhijun Luo, Ying Ying
Summary: Functional AMPKβ1 and AMPKβ2 inhibit cell proliferation and tumorigenesis in colon cancer cells, suggesting their potential as effective targets for colon cancer therapy.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2022)
Article
Pharmacology & Pharmacy
Lu Li, Huiquan Liu, Weili Tao, Su Wen, Xiaofen Fu, Shiying Yu
Summary: This study demonstrates the cachectic role of HMGB1 in colon cancer, whether it is in soluble form or secreted from tumor cells as exosomes. The HMGB1 inhibitor glycyrrhizin shows promise in relieving muscle wasting and attenuating cachexia progression.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Endocrinology & Metabolism
Severine Olivier, Camille Pochard, Hanna Diounou, Vanessa Castillo, Jordane Divoux, Joshua Alcantara, Jocelyne Leclerc, Sandra Guilmeau, Camille Huet, Wafa Charifi, Thibault V. Varin, Noemie Daniel, Marc Foretz, Michel Neunlist, Benoit L. Salomon, Pradipta Ghosh, Andre Marette, Malvyne Rolli-Derkinderen, Benoit Viollet
Summary: Intestinal AMPK plays a significant role in maintaining the integrity of distal colon IEB, but its function in regulating glucose homeostasis is limited.
MOLECULAR METABOLISM
(2021)
Article
Cell Biology
Linfei Hu, Jun Zhang, Mengran Tian, Ning Kang, Guangwei Xu, Jingtai Zhi, Xianhui Ruan, Xiukun Hou, Wei Zhang, Jiaoyu Yi, Weike Ma, Luchen Chang, Tao Tang, Xiangqian Zheng, Xi Wei, Ming Gao
Summary: The present study reveals that Ref-1 upregulation contributes to intrinsic resistance to vemurafenib in PTC. Combination treatment with a Ref-1 redox inhibitor and a BRAF inhibitor enhances the antitumor effects of vemurafenib by further inhibiting the MAPK pathway and activating excessive autophagy and related senescence process.
CELL DEATH & DISEASE
(2022)
Article
Cell Biology
Xiao-Jing Zhang, Xiaolan Liu, Manli Hu, Guo-Jun Zhao, Dating Sun, Xu Cheng, Hui Xiang, Yong-Ping Huang, Rui-Feng Tian, Li-Jun Shen, Jun-Peng Ma, Hai-Ping Wang, Song Tian, Shanyu Gan, Haibo Xu, Rufang Liao, Toujun Zou, Yan-Xiao Ji, Peng Zhang, Jingjing Cai, Zhao Wang, Guannan Meng, Qingbo Xu, Yibin Wang, Xin-Liang Ma, Peter P. Liu, Zan Huang, Lihua Zhu, Zhi-Gang She, Xin Zhang, Lan Bai, Hailong Yang, Zhibing Lu, Hongliang Li
Summary: The study found that ALOX12 exacerbates cardiomyocyte injury by not only producing 12-HETE, but also suppressing AMPK activity. Pharmacological inhibition of ALOX12 can significantly reduce cardiac injury caused by post-myocardial ischemia-reperfusion.
Review
Neurosciences
Aymeric Ravel-Chapuis, Elise Duchesne, Bernard J. Jasmin
Summary: Myotonic dystrophy type 1 (DM1) is a multisystemic disorder caused by CUG repeats in DMPK mRNAs, leading to RNA foci formation and abnormal RNA-binding proteins. AMPK activation and exercise have shown beneficial effects on the DM1 pathophysiology.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Biochemistry & Molecular Biology
Kaitlin R. Morrison, Tingting Wang, Kuan Yoow Chan, Eleanor W. Trotter, Ari Gillespie, Michael Z. Michael, Jonathan S. Oakhill, Iain M. Hagan, Janni Petersen
Summary: The expression and activity of AMPK alpha 1 catalytic subunit are significantly associated with poor prognosis in colorectal cancer patients. By attenuating mTOR control of AMPK alpha 1, a mutant colorectal cancer cell line with elevated AMPK alpha 1 signaling was generated. This elevated signaling confers hypersensitivity to metformin-induced growth inhibition, suggesting a potential role for metformin in colorectal cancer therapy.
Article
Medicine, Research & Experimental
Mohamed M. Elseweidy, Alaa S. Elawady, Mohammed S. Sobh, Gehad M. Elnagar
Summary: The study aimed to investigate the metabolic effects of lycopene (LYC) and atorvastatin (ATOR) in a diabetic hyperlipidemic rat model. The results showed that LYC had better effects on the studied biomarkers, indicating its potential as a natural therapeutic agent for hyperlipidemia.
Article
Pharmacology & Pharmacy
Lijun Kong, Hewei Zhang, Chaosheng Lu, Keqing Shi, Hongjian Huang, Yushu Zheng, Yongqiang Wang, Dan Wang, Hongwei Wang, Wei Huang
Summary: AICAR activation of AMPK significantly ameliorates PALI, with hepatic AMPK activation playing a crucial protective role in PALI. AICAR-induced AMPK activation protects rats from PALI by regulating hepatic antioxidant capacity through Nrf2 and inhibiting NLRP3 inflammasome activation.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Cell Biology
Francesca Ronca, Antonio Raggi
Summary: In this review, the interaction between TnT and AMPD is discussed. During intense muscular activity, the decrease in ATP/ADP ratio and tissue pH stimulates the activation of AMPD, which leads to the production of ATP. Proteolytic activity in skeletal muscle removes a N-terminal fragment from AMPD, resulting in reduced inhibition by ATP and increased NH3 production. TnT may mimic the regulatory function of the inhibitory N-terminal domain of AMPD, suggesting a potential association between the two proteins in modulating muscle contraction and ammonia production.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Engineering, Environmental
Juan Zhang, Wen-Hao Zhang, Christophe Morisseau, Min Zhang, Hong-Jun Dong, Qi-Meng Zhu, Xiao-Kui Huo, Cheng-Peng Sun, Bruce D. Hammock, Xiao-Chi Ma
Summary: PM2.5 is closely related to respiratory diseases and lung inflammation. sEH and epoxy fatty acids play a vital role in this inflammation. In a mouse model of PM2.5-mediated lung injury, the cytochrome P450 oxidase/sEH metabolic pathway was found to be involved. The inhibition of sEH protected against lung injury by increasing levels of EETs and inactivating pulmonary macrophages.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Geriatrics & Gerontology
Shintaro Nakamura, Yasutaka Miyachi, Akihito Shinjo, Hisashi Yokomizo, Masatomo Takahashi, Kohta Nakatani, Yoshihiro Izumi, Hiroko Otsuka, Naoichi Sato, Ryuichi Sakamoto, Takashi Miyazawa, Takeshi Bamba, Yoshihiro Ogawa
Summary: This study found that the SGLT2 inhibitor CANA can improve endurance capacity in diabetic mice and identified the potential role of the AICARP/AMPK pathway in oxidative skeletal muscles.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2023)
Article
Biochemistry & Molecular Biology
Kanlayanee Tonum, Nipitpon Srimai, Napason Chabang, Somsak Fongsupa, Patoomratana Tuchinda, Jacob A. Torres, Thomas Weimbs, Sunhapas Soodvilai
Summary: Activation of AMPK inhibits CFTR-mediated chloride secretion, leading to reduced progression of PKD. Panduratin A, as an AMPK activator, shows pharmacological effects in reducing renal cyst development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Frederic Sorgeloos, Michael Peeters, Yohei Hayashi, Fabian Borghese, Nicolas Capelli, Melissa Drappier, Teresa Cesaro, Didier Colau, Vincent Stroobant, Didier Vertommen, Gregory de Bodt, Stephane Messe, Ignasi Forne, Felix Mueller-Planitz, Jean-Francois Collet, Thomas Michiels
Summary: Microbes have evolved to exploit cellular kinases called RSKs, which play a role in defending against pathogens. Kaposi's sarcoma-associated herpesvirus and Yersinia use a similar strategy to manipulate RSKs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Isaac P. Heremans, Francesco Caligiore, Isabelle Gerin, Marina Bury, Marilena Lutz, Julie Graff, Vincent Stroobant, Didier Vertommen, Aurelio A. Teleman, Emile Van Schaftingen, Guido T. Bommer
Summary: This study reveals that PARK7, an enzyme mutated in hereditary Parkinson's disease, plays a protective role against damage caused by a metabolite of glycolysis. The findings provide important insights into the link between metabolism and the development of Parkinson's disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Neurosciences
Janne Hakanen, Nicolas Parmentier, Leonie Sommacal, Dario Garcia-Sanchez, Mohamed Aittaleb, Didier Vertommen, Libing Zhou, Nuria Ruiz-Reig, Fadel Tissir
Summary: The planar cell polarity protein Celsr3 and the microtubule depolymerizing protein Kif2a cooperatively specify the directionality of neuroblasts tangential migration in the postnatal brain, providing new insights into the mechanisms of neuroblast migration.
PROGRESS IN NEUROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Alexander I. Kostyuk, Maria-Armineh Tossounian, Anastasiya S. Panova, Marion Thauvin, Roman I. Raevskii, Daria Ezerina, Khadija Wahni, Inge Van Molle, Anastasia D. Sergeeva, Didier Vertommen, Andrey Yu. Gorokhovatsky, Mikhail S. Baranov, Sophie Vriz, Joris Messens, Dmitry S. Bilan, Vsevolod V. Belousov
Summary: In this study, we developed a fluorescent genetically encoded biosensor, Hypocrates, for the visualization of (pseudo) hypohalous acids and their derivatives. Hypocrates is ratiometric, reversible, and highly sensitive. By solving its X-ray structure, we revealed its sensing mechanism and successfully applied it in bacterial stress imaging and a zebrafish tail fin injury model.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Emna Ouni, Valerie Nedbal, Marta Da Pian, Hangbao Cao, Kalina T. Haas, Alexis Peaucelle, Olivier Van Kerk, Gaetan Herinckx, Etienne Marbaix, Marie-Madeleine Dolmans, Timo Tuuri, Marjut Otala, Christiani A. Amorim, Didier Vertommen
Summary: The increasing infertility rate in modern society has been attributed to limited knowledge of human ovarian tissue. This study reveals the importance of the extracellular side of the ovarian tissue and creates a proteomic codex for the ovarian matrisome. The research provides valuable insights into healthy ovarian physiology, disease characterization, and tissue development in vitro.
Article
Cell Biology
Sophie L. Allen, Alex P. Seabright, Jonathan Quinlan, Amritpal Dhaliwal, Felicity R. Williams, Nicholas H. F. Fine, David J. Hodson, Matthew J. Armstrong, Ahmed M. Elsharkaway, Carolyn A. Greig, Yu-Chiang Lai, Janet M. Lord, Gareth G. Lavery, Leigh Breen
Summary: This study utilized an in vitro model of liver disease to investigate the effects of serum from liver disease patients on myotube diameter, mitochondrial respiration, and muscle-related protein content. The findings suggest potential mechanisms underlying sarcopenia development in liver disease patients.
Article
Biology
Justine Dontaine, Asma Bouali, Frederic Daussin, Laurent Bultot, Didier Vertommen, Manon Martin, Raahulan Rathagirishnan, Alexanne Cuillerier, Sandrine Horman, Christophe Beauloye, Laurent Gatto, Benjamin Lauzier, Luc Bertrand, Yan Burelle
Summary: An in vitro assay in isolated heart mitochondria reveals that O-GlcNAcase inhibitor NButGT rapidly increases protein O-GlcNAcylation leading to increased respiratory capacity and complex I activity and decreased ROS release.
COMMUNICATIONS BIOLOGY
(2022)
Article
Virology
Bradley Ward, Jean Cyr Yombi, Jean-Luc Balligand, Patrice D. Cani, Jean-Francois Collet, Julien de Greef, Joseph P. Dewulf, Laurent Gatto, Vincent Haufroid, Sebastien Jodogne, Benoit Kabamba, Sebastien Pyr Dit Ruys, Didier Vertommen, Laure Elens, Leila Belkhir
Summary: This paper introduces the HYGIEIA project, which aims to study the molecular mechanisms and post-COVID conditions through a multi-omic approach and network medicine analysis. By analyzing high-throughput sequencing and mass-spectrometry data from multiple biological layers, meaningful discoveries are expected and can be translated into improvements in clinical practice.
Article
Oncology
Gilles Rademaker, Brunella Costanza, Sebastien Pyr Dit Ruys, Raphael Peiffer, Ferman Agirman, Naima Maloujahmoum, Didier Vertommen, Andrei Turtoi, Akeila Bellahcene, Vincent Castronovo, Olivier Peulen
Summary: Paladin is identified as a potential biomarker for colon cancer liver metastases, and its depletion inhibits colon cancer cell migration and metastasis.
Article
Hematology
Christian Pecquet, Nicolas Papadopoulos, Thomas Balligand, Ilyas Chachoua, Amandine Tisserand, Audrey Nedelec, Didier Vertommen, Anita Roy, Caroline Marty, Harini Nivarthi, Mira El-Khoury, Eva Hug, Andrea Majoros, Erica Xu, Oleh Zagrijtschuk, Tudor E. Fertig, Daciana S. Marta, Heinz Gisslinger, Bettina Gisslinger, Martin Schalling, Ilaria Casetti, Elisa Rumi, Daniela Pietra, Chiara Cavalloni, Luca Arcaini, Mario Cazzola, Norio Komatsu, Yoshihiko Kihara, Yoshitaka Sunami, Yoko Edahiro, Marito Araki, Roman Lesyk, Veronika Buxhofer-Ausch, Sonja Heibl, Florence Pasquier, Violaine Havelange, Isabell Plo, William Vainchenker, Robert Kralovics, Stefan N. Constantinescu
Summary: Mutant CALR proteins bind to and activate the TpoR in cells, driving the development of myeloproliferative neoplasms. These mutant CALR proteins can be found in patient plasma complexed with sTFR1, which increases their stability. They can specifically interact with TpoR on target cells and promote thrombopoietin-independent colony formation.
Article
Microbiology
Belen Lizcano-Perret, Cecile Lardinois, Fanny Wavreil, Philippe Hauchamps, Gaetan Herinckx, Frederic Sorgeloos, Didier Vertommen, Laurent Gatto, Thomas Michiels
Summary: It has been discovered that certain pathogen proteins can hijack cellular protein kinases to promote their own replication or evade immunity by redirecting them to specific substrates.
Article
Biochemistry & Molecular Biology
Laura Glorieux, Laura Vandooren, Sylvie Derclaye, Sebastien Pyr Dit Ruys, Paloma Oncina-Gil, Anna Salowka, Gaetan Herinckx, Elias Aajja, Pascale Lemoine, Catherine Spourquet, Helene Lefort, Patrick Henriet, Donatienne Tyteca, Francesca M. Spagnoli, David Alsteens, Didier Vertommen, Christophe E. Pierreux
Summary: The study identified and quantified the extracellular matrix (ECM) composition of the developing pancreas using mass spectrometry and found that it displayed dynamic expression profiles with changes in collagen and proteoglycans. Atomic force microscopy measurements showed that the pancreatic ECM was soft and the biomechanical properties did not significantly change during pancreas maturation. The study also optimized a decellularization protocol for pancreatic tissues, preserving their 3D ECM organization and proving suitable for recellularization studies. These findings provide insights into the ECM composition and biomechanics of the pancreatic embryonic and perinatal stages, laying the groundwork for further investigations on the interactions between the ECM and pancreatic cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Svitlana Drozdovska, Nadege Zanou, Jessica Lavier, Lucia Mazzolai, Gregoire P. Millet, Maxime Pellegrin
Summary: This study investigated the effect of low- and supramaximal-intensity hypoxic training on muscle metabolic gene expression in mice. The results indicated that hypoxic training, regardless of exercise intensity, has a moderate effect on muscle metabolic gene expression in healthy mice.
Article
Geriatrics & Gerontology
Jeremy Vidal, Eric A. Fernandez, Martin Wohlwend, Pirkka-Pekka Laurila, Andrea Lopez-Mejia, Julien Ochala, Alexander J. Lobrinus, Bengt Kayser, Isabel C. Lopez-Mejia, Nicolas Place, Nadege Zanou
Summary: The study reveals that decreased RyR1 protein levels are commonly observed in various myopathies and are associated with endoplasmic reticulum (ER) stress, suggesting the important role of RyR1 depletion-induced ER stress in the pathogenesis of myopathies.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2023)
Article
Biochemistry & Molecular Biology
Samuel O. Lord, Yu-Chiang Lai
Summary: Researchers conducted the first global analysis of exercise-regulated ubiquitin signaling, revealing the potential role of NEDDylation in the removal of damaged proteins after exercise. The proteomic dataset generated from this study is invaluable for validating new mechanistic hypotheses.
