Article
Chemistry, Medicinal
Keisuke Komatsuya, Takaya Sakura, Kazuro Shiomi, Satoshi Omura, Kenji Hikosaka, Tomoyoshi Nozaki, Kiyoshi Kita, Daniel Ken Inaoka
Summary: The mitochondrial electron transport chain of Plasmodium falciparum has been identified as a promising target for chemotherapy, with two enzymes and complex II considered as candidate drug targets. Siccanin has been discovered as a potent inhibitor of complex II and III in P. falciparum, showing no inhibition of corresponding complexes in mammalian mitochondria. This compound has a dual mechanism of action and is a potential candidate for the development of new antimalarial drugs.
Article
Plant Sciences
Yovanny Izquierdo, Luis Muniz, Jorge Vicente, Satish Kulasekaran, Veronica Aguilera, Ana Lopez Sanchez, Ada Martinez-Ayala, Bran Lopez, Tomas Cascon, Carmen Castresana
Summary: Plant oxylipins are signaling molecules produced from fatty acids by oxidative pathways, primarily initiated by 9- and 13-lipoxygenases, impacting plant development and defense mechanisms. Studies on mutant plants insensitive to specific oxylipin products indicate a close connection between oxylipin signaling and mitochondrial stress responses in plants.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Pharmacology & Pharmacy
Mohammed Saarti, Hani Almukhtar, Paul A. Smith, Richard E. Roberts
Summary: The inhibitors of mitochondrial complex III, antimycin A and myxothiazol, suppress contractile responses in porcine isolated coronary arteries possibly by inhibiting calcium influx pathways and activating the AMP kinase pathway.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Biology
Abhilash Padavannil, Anjaneyulu Murari, Shauna-Kay Rhooms, Edward Owusu-Ansah, James A. Letts
Summary: Respiratory complex I plays a crucial role in bioenergetic metabolism and shows differences in behavior between Protostomia and Deuterostomia. The structure and conformational states of Drosophila melanogaster complex I provide insight into its functions and mechanisms in different organisms.
Article
Oncology
Martyn A. Sharpe, David S. Baskin, Kumar Pichumani, Omkar B. Ijare, Santosh A. Helekar
Summary: EMF can raise ROS levels in cells, potentially toxic to cancer cells; weak magnetic fields can disrupt electron flow in mitochondrial ETC, inducing cell death; sOMF of specific strength and frequency can completely halt electron transport and lead to mitochondrial dysfunction and cell death.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biology
Natalia Belosludtseva, Vlada S. Starinets, Alena A. Semenova, Anastasia D. Igoshkina, Mikhail Dubinin, Konstantin N. Belosludtsev
Summary: The metabolic agent S-15176 difumarate salt affects mitochondrial function by disturbing the catalytic function of enzyme complex III and inducing nonspecific membrane permeability. It leads to the dissipation of the mitochondrial membrane potential and a decline in ATP production. These findings suggest that S-15176 can induce mitochondrial dysfunction in mammal tissues and organs that are most vulnerable to chemical toxicity or exposed to higher concentrations of the drug.
Article
Biology
C. J. Kelly, Reid K. Couch, Vivian T. Ha, Camille M. Bodart, Judy Wu, Sydney Huff, Nicole T. Herrel, Hyunsung D. Kim, Azaad O. Zimmermann, Jessica Shattuck, Yu-Chen Pan, Matt Kaeberlein, Anthony S. Grillo
Summary: Mice lacking Complex I subunit NDUFS4 exhibit mitochondrial dysfunction and the abnormal iron homeostasis may contribute to the progression of Leigh Syndrome and other mitochondrial disorders.
Article
Urology & Nephrology
Nan Guan, Hanako Kobayashi, Ken Ishii, Olena Davidoff, Feng Sha, Talat A. Ikizler, Chuan-Ming Hao, Navdeep S. Chandel, Volker H. Haase
Summary: This study investigates the role of mitochondrial electron transport in kidney homeostasis by inactivating a subunit of mitochondrial complex III in kidney progenitor cells. The results show that mitochondrial oxidative metabolism is critical for kidney development, and inactivation of specific proteins can lead to severe kidney dysplasia or smaller functional kidneys.
KIDNEY INTERNATIONAL
(2022)
Article
Cell Biology
Xuan Wang, Yuting Ji, Jingyi Qi, Shuaishuai Zhou, Sitong Wan, Chang Fan, Zhenglong Gu, Peng An, Yongting Luo, Junjie Luo
Summary: Cervical cancer is a deadly disease in women, and mitochondrial-mediated ferroptosis (MMF) has been identified as a key factor in its development. By investigating the mitochondria transmembrane candidates, researchers discovered that mitochondrial carrier 1 (MTCH1) plays a central role in MMF in cervical cancers. MTCH1 deficiency disrupted mitochondrial oxidative phosphorylation and increased mitochondrial reactive oxygen species (ROS), leading to ferroptosis. Targeting MTCH1 in combination with Sorafenib effectively inhibited cervical cancer growth by inducing ferroptosis in a mouse xenograft model. These findings provide insights into the mechanisms of MMF and offer a potential therapeutic target for cervical cancer.
CELL DEATH & DISEASE
(2023)
Article
Cell Biology
Xiphias Ge Zhu, Aleksey Chudnovskiy, Lou Baudrier, Benjamin Prizer, Yuyang Liu, Benjamin N. Ostendorf, Norihiro Yamaguchi, Abolfozl Arab, Bernardo Tavora, Rebecca Timson, Soren Heissel, Elisa de Stanchina, Henrik Molina, Gabriel D. Victora, Hani Goodarzi, Kivanc Birsoy
Summary: This study reveals that loss of heme synthesis reduces tumor growth in PDAC, and autophagy plays a crucial role in immune evasion of pancreatic tumors.
Article
Biochemistry & Molecular Biology
Maria Kawalec, Piotr Wojtyniak, Ewelina Bielska, Anita Lewczuk, Anna Boratynska-Jasinska, Malgorzata Beresewicz-Haller, Malgorzata Frontczak-Baniewicz, Magdalena Gewartowska, Barbara Zablocka
Summary: Transient ischemic attacks (TIA) occur due to temporary blockage of blood circulation in the brain. This study found that changes in mitochondrial dynamics, mitophagy, and biogenesis may contribute to a reduced susceptibility to TIA.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Lianne R. de Haan, Megan J. Reiniers, Laurens F. Reeskamp, Ali Belkouz, Lei Ao, Shuqun Cheng, Baoyue Ding, Rowan F. van Golen, Michal Heger
Summary: This study analyzed the influence of ancillary factors on the formation of DCF using DCFH2-DA in the context of ETC inhibitors. The results showed that the type of culture medium, pH of the assay system, presence of fetal calf serum, and DCFH2-DA solvent concentration affect the formation of DCF. Hence, proper controls should be included in experiments using DCFH2-DA.
Article
Multidisciplinary Sciences
Mahmoud A. Bassal, Saumya E. Samaraweera, Kelly Lim, Brooks A. Bernard, Sheree Bailey, Satinder Kaur, Paul Leo, John Toubia, Chloe Thompson-Peach, Tran Nguyen, Kyaw Ze Ya Maung, Debora A. Casolari, Diana G. Iarossi, Ilaria S. Pagani, Jason Powell, Stuart Pitson, Siria Natera, Ute Roessner, Ian D. Lewis, Anna L. Brown, Daniel G. Tenen, Nirmal Robinson, David M. Ross, Ravindra Majeti, Thomas J. Gonda, Daniel Thomas, Richard J. D'Andrea
Summary: This study reveals the mutual exclusivity between germline mutations in mitochondrial complex I and somatic mutations in the metabolic enzyme IDH1 in patients with acute myeloid leukemia (AML), and finds that IDH1 mutant cells have increased sensitivity to complex I inhibitors.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Ami Kobayashi, Toshihiko Takeiwa, Kazuhiro Ikeda, Satoshi Inoue
Summary: The mitochondrial electron transport chain (ETC) is crucial for energy production and drives various biochemical processes in eukaryotic cells through oxidative phosphorylation (OXPHOS). Recent studies have shown that noncoding RNAs (ncRNAs), including microRNAs (miRNAs), transfer-RNA-derived fragments (tRFs), long ncRNAs (lncRNAs), and circular RNAs (circRNAs), play important roles in regulating ETC and OXPHOS systems. This review highlights the emerging roles of ncRNAs in the regulation of mitochondrial ETC and OXPHOS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Qun Chen, Jeremy Thompson, Ying Hu, Edward J. Lesnefsky
Summary: Aging-induced endoplasmic reticulum (ER) stress impairs mitochondrial function, which can be improved by attenuating ER stress or inhibiting calpain 1 (CPN1). These interventions can decrease cardiac injury during ischemia-reperfusion in aged hearts.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jianmei Hou, Elizabeth Nguyen, Minglyanna Surudoi, Michael J. Roy, Onisha Patel, Isabelle S. Lucet, Xiuquan Ma, Roger J. Daly
Summary: This article investigates the regulation and role of pseudokinase scaffolds PEAK1 and PEAK2 in cell signaling. The study finds that PEAK3, the third family member, also plays a crucial role in promoting cell proliferation, migration, and invasion by interacting with various proteins.
Article
Chemistry, Physical
Tianyu Xu, Shaowei Zhou, Xiuquan Ma, Han Wu, Lin Zhang, Meng Li
Summary: Laser welding of 2A12 aluminum alloy with added carbon nanotubes improves welding efficiency and significantly enhances the tensile strength of joints. The introduction of carbon nanotubes reduces pore size and improves mechanical properties. Increased precipitated phases increase dislocation density and provide a basis for dislocation strengthening. Carbon nanotubes are tightly bonded to the aluminum matrix, improving load transfer.
Article
Engineering, Industrial
Lin Zhang, Han Wu, Jincheng Wen, Meng Li, Xinyu Shao, Xiuquan Ma
Summary: This paper proposes a novel method using a femtosecond laser to join Al6082 and SiO2, which involves drilling, ejection, filling, and solidification to form a mechanical pin structure ensuring tight bonding between the two materials. The proposed method substantially reduces the requirements of material surface roughness and achieves over 100 MPa shear strength, comparable to the tensile strength of the aluminum alloy. It provides a new technique to improve product quality and facilitate the manufacturing process for joining dissimilar materials.
JOURNAL OF MATERIALS PROCESSING TECHNOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Chang Hu, Chunming Wang, Xiuquan Ma, Zhengwu Zhu, Ping Jiang, Gaoyang Mi
Summary: In this study, the evolution of microstructures and performance variations during laser-arc welding of 316L austenitic stainless steel were analyzed under the influence of a magnetic field. The magnetic field was found to refine austenite grains, suppress ferrite dendrite growth, and enhance the tensile strength of the material. The current, supercooling, and electromagnetic stirring were also investigated at different magnetic intensities, showing different effects on the microstructure evolution.
MATERIALS & DESIGN
(2022)
Article
Optics
Chang Hu, Chunming Wang, Shaowei Zhou, Han Wu, Kexiong Sun, Xiuquan Ma
Summary: A new manufacturing method for an all-fiber signal combiner based on CO2 laser ablation is proposed, which avoids the tapering process and results in better beam quality compared to the traditional method.
OPTICS AND LASER TECHNOLOGY
(2022)
Article
Optics
Kexiong Sun, Shicheng Zhu, Li Li, Chang Hu, Xiuquan Ma
Summary: This paper presents numerical studies on twisted polygon-shaped core fibers for achieving single mode operation with a large mode area propagation. Twisting-induced high losses to higher order modes and coupling higher order modes with leaky modes are effective for achieving single mode operation in large mode area fibers.
Article
Physics, Multidisciplinary
Yichun Wang, Han Wu, Wenkang Lu, Meng Li, Ling Tao, Xiuquan Ma
Summary: The study on ionizing normal saline induced by femtosecond laser focused on gas production rate and composition, revealing that each laser pulse can independently generate ionization gas when the repetition rate is less than 1000 Hz. Additionally, it was found that meglumini diatrizoici has an inhibitory effect on the ionization process, with the reasons for this inhibition explained. Lastly, analysis of the gas composition confirmed that the primary gas production mechanism of the femtosecond laser is the decomposition of water molecules, while the composition of the solution after the reaction proved the decomposition effect of the laser on meglumine.
Article
Materials Science, Multidisciplinary
Tianyu Xu, Xiuquan Ma, Lin Zhang, Meng Li, Shaowei Zhou, Han Wu, Zhengwu Zhu
Summary: In this paper, carbon nanotubes (CNTs) and silicon nitride (Si3N4) were used to form composite Al-Cu-Mg joints by laser welding. Results showed that the CNTs were either dispersedly arranged in the fusion zone or tightly coupled with nano-scale Si3N4 and Mg2Si precipitates. Coherent interfaces were built between CNTs and surroundings. The microstrain transfer and transformation between the Al-Cu-Mg matrix and the CNTs were mainly attributed to the formation of Al4C3. The tensile strength and elongation rate of the joint with composite additives were significantly improved compared to single laser welding. This work provides a new idea for optimizing the bonding design of the interface between CNTs and aluminum matrix to improve load transfer efficiency.
MATERIALS CHARACTERIZATION
(2022)
Article
Materials Science, Multidisciplinary
Tianyu Xu, Zhengwu Zhu, Gaoyang Mi, Libo Wang, Meng Li, Xiuquan Ma
Summary: In-situ addition of carbon nanotubes (CNTs) was used to strengthen laser welded Al-Cu-Mg alloys, and the mechanisms of CNTs strengthening joints were revealed. The CNTs were found to combine with precipitates and disperse in the matrix, providing conditions for load transfer. The fusion zone (FZ) grains of the CNTs-reinforced joints were finer and isotropic compared to joints without CNTs. The thermal mismatches between CNTs and aluminum alloys, as well as the role of CNTs as grain boundaries, contributed to the significant improvements in CNTs-reinforced joints.
MATERIALS CHARACTERIZATION
(2022)
Article
Optics
Chang Hu, Chunming Wang, Shaowei Zhou, Zhengwu Zhu, Han Wu, Kexiong Sun, Xiuquan Ma
Summary: This study proposes a new method for manufacturing D-shaped optical fibers using CO2 laser ablation technology. Numerical modeling and experimental analysis were conducted to understand the surface morphology formation mechanism and the impact of laser parameters on processing quality and efficiency.
OPTICS AND LASER TECHNOLOGY
(2022)
Article
Cell Biology
Camila de Castro Ferezin, Terry C. C. Lim Kam Sian, Yunjian Wu, Xiuquan Ma, Anderly C. Chueeh, Cheng Huang, Ralf B. Schittenhelm, Jorg Kobarg, Roger J. Daly
Summary: In this study, we found that NEK5 has diverse effects on signaling pathways and biological processes in breast epithelial cells, and may play an important role in the development and progression of breast cancer.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Biochemical Research Methods
Kexiong Sun, Hongzhang He, Xinyu Xia, Han Wu, Ling Tao, Xiuquan Ma
Summary: This paper investigates the burst mode laser ablation of porcine subcutaneous fat and porcine aorta, showing the potential for selective tissue ablation. The results demonstrate that subcutaneous fat can be ablated at a rate of over 0.2 mm/s with a fluence of 0.12 mJ/mm(2) per pulse and 180 mJ/mm(2) per burst, while the porcine aorta remains unaffected. The tissue temperature during lasing is lower than 45 degrees C for subcutaneous fat and 35 degrees C for the aorta.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Materials Science, Multidisciplinary
Shuye Zheng, Yu Huang, Chunming Wang, Zhengwu Zhu, Xiuquan Ma, Ping Jiang, Gaoyang Mi
Summary: This study comparatively investigated the melting-solidifying and heat-affected zones of 316 L stainless steel plates through laser-arc welding, laser welding, and laser cutting. The morphology, crystalline orientation, and phase interfaces were systematically analyzed. The refinement or coarsening of 8 and gamma phases in the zones were attributed to solid/liquid interface instability, lowered high-temperature duration, and variable epitaxial growth.
MATERIALS CHARACTERIZATION
(2022)
Article
Biochemistry & Molecular Biology
Xue Yang, Miguel I. Cruz, Elizabeth V. Nguyen, Cheng Huang, Ralf B. Schittenhelm, Jennii Luu, Karla J. Cowley, Sung-Young Shin, Lan K. Nguyen, Terry C. C. Lim Kam Sian, Kimberley C. Clark, Kaylene J. Simpson, Xiuquan Ma, Roger J. Daly
Summary: We found that the expression of pseudokinase NRBP1 is associated with poor prognosis in triple negative breast cancer (TNBC). NRBP1 is required for efficient migration, invasion and proliferation of TNBC cells in culture, as well as growth of TNBC orthotopic xenografts and experimental metastasis. NRBP1 interacted with P-Rex1, Rac1, and Cdc42, and its overexpression enhanced levels of GTP-bound Rac1 and Cdc42 in a P-Rex1-dependent manner, leading to increased cell migration and invasion.
Article
Optics
Xiuquan Ma, Libo Wang, Zhengwu Zhu, Lei Su, Wei Guo, Gaoyang Mi, Tianyu Xu
Summary: In this paper, a novel welding technology using laser in a planetary system was proposed. The crystal orientation and growth features in the fusion zones were studied and compared to single laser welding. The planetary system laser welding showed enhanced nucleation and transformation of growth, resulting in increased grain misorientation and decreased aspect ratio and grain size.
OPTICS AND LASER TECHNOLOGY
(2023)
