☆ 4.1 Article

Photocleavage of the Polypeptide Backbone by 2-Nitrophenylalanine

CHEMISTRY & BIOLOGY (2009)

期刊

CHEMISTRY & BIOLOGY

卷 16, 期 2, 页码 148-152

出版社

CELL PRESS

DOI: 10.1016/j.chembiol.2009.01.013

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Biochemistry & Molecular Biology

资金

NIH [R01 GM62159]

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Reagent

摘要

Photocleavage of the polypeptide backbone is potentially a powerful and general method to activate or deactivate functional peptides; and proteins with high spatial and temporal resolution. Here we show that 2-nitrophenylalanine is able to photochemically cleave the polypeptide backbone by an unusual cinnoline-forming reaction. This unnatural amino acid was genetically encoded in E coli, and protein containing 2-nitrophenylalanine was expressed and site-specifically photocleaved.

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