期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 54, 期 45, 页码 13230-13235出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201505138
关键词
electron microscopy; fluorescent probes; immunochemistry; NMR spectroscopy; single-molecule studies
资金
- German Funding Agency (DFG) [SFB958/Z02, SFB958/A01]
The precision of single-molecule localization-based super-resolution microscopy, including dSTORM, critically depends on the number of detected photons per localization. Recently, reductive caging of fluorescent dyes followed by UV-induced recovery in oxidative buffer systems was used to increase the photon yield and thereby the localization precision in single-color dSTORM. By screening 39dyes for their fluorescence caging and recovery kinetics, we identify novel dyes that are suitable for multicolor caged dSTORM. Using a dye pair suited for registration error-free multicolor dSTORM based on spectral demixing (SD), a multicolor localization precision below 15nm was achieved. Caged SD-dSTORM can resolve the ultrastructure of single 40nm synaptic vesicles in brain sections similar to images obtained by immuno-electron microscopy, yet with much improved label density in two independent channels.
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