期刊
CHEMICO-BIOLOGICAL INTERACTIONS
卷 175, 期 1-3, 页码 73-75出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.cbi.2008.02.006
关键词
Acetylcholinesterase; Sulfhydryl reagents; Aphids; Insecticides
资金
- NIGMS NIH HHS [R15 GM072510, R15 GM072510-01, 1R15 GM 072510-01] Funding Source: Medline
Previously we used site-directed mutagenesis, in vitro expression, and molecular modeling to investigate the inactivation of an invertebrate acetylcholinesterase, cholinesterase 2 from amphioxus, by the Sulfhydryl reagents 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) and N-ethylmaleimide (NEM). We created the mutants C310A, C466A, C310A/C466A and C310A/F3121 to assess the roles of the two cysteines and a proposal that the increased rate of inactivation previously found in an F3121 mutant was due to increased access of sulfhydryl reagents to Cys310. Our results indicated that both of the cysteines could be involved in inactivation by sulfhydryl reagents, but that the cysteine near the acyl pocket was more accessible. We speculated that the inactivation of aphid AChEs by sulfhydryl reagents was due to the presence of a cysteine homologous to Cys310 and proposed that this residue could be a target for a specific insecticide. Here we reconsider this proposal. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
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