Article
Environmental Sciences
Dongpeng Li, Yifei Wang, Xinshan Song, Mengqi Jiang, Xiaoxiang Zhao, Xin Cao
Summary: Harmful algal blooms (HABs) have detrimental effects on the aquatic ecosystem and pose risks to human health and economic losses. Photocatalytic inhibition of algae is gaining more attention, but the impact of intense light irradiation on algal growth has been largely overlooked. Our study found that intense light irradiation significantly affected the physiological activity of algal cells and inhibited photosynthesis, which is crucial for the study of photocatalytic removal of harmful algae.
Article
Biotechnology & Applied Microbiology
Neil D. Butler, Shelby R. Anderson, Roman M. Dickey, Priyanka Nain, Aditya M. Kunjapur
Summary: Aldehydes are important chemical targets for flavors and fragrances industry and synthetic intermediates due to their ability to form C-C bonds. This study identifies and addresses unexpected oxidation of aromatic aldehydes, and demonstrates that it can be slowed down by inactivating certain aldehyde dehydrogenase genes in E. coli. The newly engineered E. coli ROAR strain shows reduced oxidation and improved product titers in biocatalysis reactions involving aldehydes.
METABOLIC ENGINEERING
(2023)
Article
Multidisciplinary Sciences
Marco Orecchioni, Kouji Kobiyama, Holger Winkels, Yanal Ghosheh, Sara McArdle, Zbigniew Mikulski, William B. Kiosses, Zhichao Fan, Lai Wen, Yunmin Jung, Payel Roy, Amal J. Ali, Yukiko Miyamoto, Matthew Mangan, Jeffrey Makings, Zhihao Wang, Angela Denn, Jenifer Vallejo, Michaela Owens, Christopher P. Durant, Simon Braumann, Navid Mader, Lin Li, Hiroaki Matsunami, Lars Eckmann, Eicke Latz, Zeneng Wang, Stanley L. Hazen, Klaus Ley
Summary: This study found that mouse vascular macrophages express the olfactory receptor Olfr2, which can detect and activate the inflammasome, leading to interleukin-1b secretion. It was also discovered that human and mouse blood plasma contains octanal, which can activate Olfr2 and its human ortholog OR6A2. Increasing octanal levels exacerbates atherosclerosis, while targeting Olfr2 gene significantly reduces atherosclerotic plaques. These findings suggest that inhibiting OR6A2 may be a promising strategy for preventing and treating atherosclerosis.
Article
Biochemistry & Molecular Biology
Felix Wong, Jonathan M. Stokes, Sarah C. Bening, Charles Vidoudez, Sunia A. Trauger, James J. Collins
Summary: This study found that bactericidal antibiotics still have killing effect under anaerobic conditions. By affecting the energy demands of cells, antibiotics generate reactive metabolic byproducts, particularly reactive electrophilic species, which leads to bacterial death.
Review
Biochemistry & Molecular Biology
Burak Mugdat Karan, Karis Little, Josy Augustine, Alan W. Stitt, Tim M. Curtis
Summary: Diabetic retinopathy (DR) is a complication of diabetes mellitus that can cause vision loss and blindness. This review article explores the impact of oxidative stress, lipid-derived aldehydes, and advanced lipoxidation end products (ALEs) on the progression of DR. It also highlights the importance of aldehyde metabolism in the retina, particularly the role of aldehyde dehydrogenase (ALDH) and aldo-keto reductase (AKR) enzymes. The review discusses potential therapeutic strategies, such as gene therapy and pharmacological compounds, that can enhance the expression and activity of ALDH and AKR enzymes to protect the retina and preserve vision in diabetic patients.
Review
Oncology
Vasileios Xanthis, Theodora Mantso, Anna Dimtsi, Aglaia Pappa, Vasiliki E. Fadouloglou
Summary: Cancer is a leading cause of death globally, and its incidence rates are expected to increase in the coming years. Human aldehyde dehydrogenases (hALDHs) are enzymes responsible for oxidation reactions in the body and have been found to be associated with cancer pathology. This review provides a detailed understanding of their functions, 3D structures, and their roles in cancer development and chemotherapy resistance. It also discusses the impact of structural features and changes on their function and ability to interact with other proteins.
Article
Multidisciplinary Sciences
Monika Groth, Wojciech Luczaj, Justyna Dunaj-Malyszko, Elzbieta Skrzydlewska, Anna Moniuszko-Malinowska
Summary: Tick-borne encephalitis (TBE) is a viral disease that is still not fully understood. This study evaluated changes in plasma lipid profiles in TBE patients and patients with bacterial co-infections. The results showed that specific lipid species can distinguish TBE patients from those with co-infections. The study also found significant alterations in fatty acid metabolism.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Physical
Hong-Xu Lei, KaiLi Zhang, Yu-Xi Qin, Rong-Jian Dong, De-Zhan Chen, HaiFeng Zhou, Xie-Huang Sheng
Summary: Ferroptosis is a type of regulated necrosis characterized by iron-dependent accumulation of lipid hydroperoxides, with potential involvement in cardiovascular disease and neurodegeneration. The discovery of tetrahydroquinoxaline as a novel scaffold of ferroptosis inhibitors shows promising inhibitory effects without cytotoxicity in human cancer cells. Its small molecular weight allows for conjugation with other drugs to form multi-target drugs for the treatment of complex diseases.
JOURNAL OF MOLECULAR STRUCTURE
(2021)
Article
Biochemistry & Molecular Biology
Yang Liu, Yuan Wang, Zhi Lin, Rui Kang, Daolin Tang, Jiao Liu
Summary: SLC25A22 acts as a metabolic repressor of ferroptosis by producing glutathione and monounsaturated fatty acids, providing a previously unrecognized defense pathway against ferroptotic cell death.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Biochemistry & Molecular Biology
Cristina Mas-Bargues, Consuelo Escriva, Mar Dromant, Consuelo Borras, Jose Vina
Summary: Oxidative stress, caused by an imbalance between prooxidants and antioxidants, can lead to biomolecular damage and development of diseases. Lipids are sensitive to oxidant attack, with MDA, 4-HNE, and F2-isoprostane being the main biomarkers for lipid peroxidation assessment. The profile of PUFAs in tissues determines the proportion of each biomarker.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Neurosciences
Sean K. Ryan, Matija Zelic, Yingnan Han, Erin Teeple, Luoman Chen, Mahdiar Sadeghi, Srinivas Shankara, Lilu Guo, Cong Li, Fabrizio Pontarelli, Elizabeth H. Jensen, Ashley L. Comer, Dinesh Kumar, Mindy Zhang, Joseph Gans, Bailin Zhang, Jonathan D. Proto, Jacqueline Saleh, James C. Dodge, Virginia Savova, Deepak Rajpal, Dimitry Ofengeim, Timothy R. Hammond
Summary: Iron dysregulation is associated with multiple neurodegenerative diseases, including Parkinson's disease. In this study, it is found that iron-loaded microglia are responsive to iron and susceptible to ferroptosis, a form of cell death that is dependent on iron. Iron overload also induces a shift in the microglial transcriptional state that is similar to the transcriptomic signature found in postmortem brain microglia of Parkinson's disease. The removal of microglia delays iron-induced neurotoxicity, suggesting the contribution of microglial response to neurodegeneration.
NATURE NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Raul Arcusa, Juan Angel Carillo, Begona Cerda, Thierry Durand, Angel Gil-Izquierdo, Sonia Medina, Jean-Marie Galano, Maria Pilar Zafrilla, Javier Marhuenda
Summary: This study aimed to evaluate the efficacy of long-term consumption of high polyphenolic content from fruits and vegetables (such as berries) as a nutraceutical against central nervous system lipid peroxidation, as assessed through urine biomarkers. The results showed significant reductions in AdA and DHA-derived metabolites after consumption of the nutraceutical, indicating its effectiveness in preventing CNS lipid peroxidation.
Article
Biochemistry & Molecular Biology
Nathchar Naowarojna, Tianshu William Wu, Zijian Pan, Mengyu Li, Jingrong Regina Han, Yilong Zou
Summary: Ferroptosis is characterized by the accumulation of polyunsaturated-lipid peroxidation on cellular membranes in an iron-dependent manner. It has been implicated in various pathophysiological processes, but our understanding of its regulation is incomplete.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Gastroenterology & Hepatology
Ninh Quoc Dat, Le Thi Thanh Thuy, Vu Ngoc Hieu, Hoang Hai, Dinh Viet Hoang, Nguyen Thi Thanh Hai, Tuong Thi Van Thuy, Tohru Komiya, Krista Rombouts, Minh Phuong Dong, Ngo Vinh Hanh, Truong Huu Hoang, Misako Sato-Matsubara, Atsuko Daikoku, Chiho Kadono, Daisuke Oikawa, Katsutoshi Yoshizato, Fuminori Tokunaga, Massimo Pinzani, Norifumi Kawada
Summary: Cytoglobin (CYGB) expressed in hepatic stellate cells has antifibrotic properties, attenuating liver fibrosis, inflammation, and oxidative cell damage. Intravenous injection of CYGB effectively suppresses drug-induced liver injury, showing promising antifibrotic effects in cell culture and animal models.
Article
Biochemistry & Molecular Biology
Jessica L. H. Walters, Amanda L. Anderson, Sarah J. Martins da Silva, R. John Aitken, Geoffry N. De Iuliis, Jessie M. Sutherland, Brett Nixon, Elizabeth G. Bromfield
Summary: Overproduction of reactive oxygen species resulting in lipid peroxidation and the production of cytotoxic reactive carbonyl species such as 4-hydroxynonenal is a prevalent cause of sperm dysfunction in male infertility patients. ALOX15 plays a key role in this pathway in human spermatozoa, with increased ALOX15 abundance leading to membrane lipid peroxidation and cellular dysfunction, ultimately contributing to male infertility.
Article
Biochemistry & Molecular Biology
Luis Francisco Calleja, Javier Alejandro Belmont-Diaz, Oscar Medina-Contreras, Hector Quezada, Belem Yoval-Sanchez, Jesus Campos-Garcia, Jose Salud Rodriguez-Zavala
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2020)
Article
Oncology
Mayra Xochitl Duran-Maldonado, Laura Hernandez-Padilla, Juan Carlos Gallardo-Perez, Alma Laura Diaz-Perez, Lorena Martinez-Alcantar, Homero Reyes De la Cruz, Jose Salud Rodriguez-Zavala, Gustavo Pacheco-Rodriguez, Joel Moss, Jesus Campos-Garcia
FRONTIERS IN ONCOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Javier Porras-Ramirez, Rosa Estrada-Reyes, Jose Salud Rodriguez-Zavala, Ana Maria Dorantes-Barron, Noe Jurado-Hernandez, Mariano Martinez-Vazquez
Summary: This study aimed to synthesize novel antidepressant-like agents and evaluate their effects in mice, with compound (2-bromophenyl)-(3,4-dihydroisoquinoline-1-yl) methanone (4a) showing the most potent effects. Antagonist experiments indicated that 4a acted on serotonin and dopamine receptors, with docking studies showing a strong affinity with the human dopamine D3 receptor. This study highlights the pharmacological potential of halogenated BIQs in treating depressive disorders.
CANADIAN JOURNAL OF CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Luis Francisco Calleja, Belem Yoval-Sanchez, Luz Hernandez-Esquivel, Juan Carlos Gallardo-Perez, Marcela Sosa-Garrocho, Alvaro Marin-Hernandez, Ricardo Jasso-Chavez, Marina Macias-Silva, Jose Salud Rodriguez-Zavala
Summary: This study demonstrates the cell protection potential of omeprazole in eukaryotic cells by preserving the activity of aldehyde dehydrogenases (ALDHs) and reducing lipid aldehyde levels and oxidative stress. The results highlight the important role of ALDHs as part of the antioxidant system of the cell in preserving cell viability under stress conditions.
Article
Biotechnology & Applied Microbiology
L. Aguilar-Vega, L. E. Lopez-Jacome, B. Franco, S. Munoz-Carranza, N. Vargas-Maya, R. Franco-Cendejas, M. Hernandez-Duran, M. Otero-Zuniga, C. Campo-Beleno, J. G. Jimenez-Cortes, M. Martinez-Vazquez, J. S. Rodriguez-Zavala, T. Maeda, R. Zurabian, R. Garcia-Contreras
Summary: The study demonstrates that phenothiazine derivatives, especially chlorpromazine, exhibit attractive antibacterial properties against nosocomial multidrug-resistant strains of A. baumannii by generating reactive oxygen species (ROS) and causing cell membrane and DNA damage. Chlorpromazine also shows synergy with various antibiotics and is effective in treating experimentally infected Galleria mellonella when combined with ceftazidime. Repurposing phenothiazine derivatives for treating resistant infections by A. baumannii shows promising potential.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Humberto Cortes-Lopez, Javier Castro-Rosas, Rodolfo Garcia-Contreras, Jose Salud Rodriguez-Zavala, Bertha Gonzalez-Pedrajo, Miguel Diaz-Guerrero, Javier Hernandez-Morales, Naybi Munoz-Cazares, Marcos Soto-Hernandez, Lucero del Mar Ruiz-Posadas, Israel Castillo-Juarez
Summary: The study found that extracts from Hibiscus sabdariffa L. calyxes and two gamma-lactones have the potential to inhibit the quorum sensing system of Pseudomonas aeruginosa and reduce bacterial virulence. Particularly, hibiscus acid showed promise in preventing infections caused by P. aeruginosa.
JOURNAL OF MEDICINAL FOOD
(2021)
Article
Biochemistry & Molecular Biology
Fariha Ansari, Belem Yoval-Sanchez, Zoya Niatsetskaya, Sergey Sosunov, Anna Stepanova, Christian Garcia, Edward Owusu-Ansah, Vadim Ten, Ilka Wittig, Alexander Galkin
Summary: Accurate measurement of the content of mitochondrial complex I and alpha-ketoglutarate dehydrogenase complex is crucial for disease research and evaluation of treatment efficacy.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
R. Daniela Feregrino-Mondragon, Alicia Vega-Segura, Rosina Sanchez-Thomas, Mayel Silva-Flores, Jose S. Rodriguez-Zavala, Alvaro Marin-Hernandez, Israel Perez-Torres, M. Eugenia Torres-Marquez, Rafael Moreno-Sanchez, Ricardo Jasso-Chavez
Summary: The study assessed the role of Euglena gracilis mitochondrial metabolism in degrading organic matter present in liquid waste. It found that the cells can actively consume amino acids in waste supernatants and utilize organic waste as a carbon source for synthesizing biotechnological relevant metabolites. Mitochondria from waste supernatant grown cells exhibited similar activities and capabilities to those from glutamate+malate grown cells.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Review
Biochemistry & Molecular Biology
Alvaro Marin-Hernandez, Jose S. Rodriguez-Zavala, Ricardo Jasso-Chavez, Emma Saavedra, Rafael Moreno-Sanchez
Summary: Protein acetylation is a widespread biological process found in non-nuclear proteins, potentially regulating metabolic pathways. However, there is low correlation between acetylation levels and enzyme activity/pathway fluxes, and over-interpretation of results from mutant enzymes mimicking acetylation is common. Rigorous enzyme kinetic analysis in parallel with acetylation level determinations is often lacking.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Article
Biochemical Research Methods
Fariha Ansari, Belem Yoval, Zoya Niatsetskaya, Vadim Ten, Ilka Wittig, Alexander Galkin
Summary: This study introduces a novel method for measuring the absolute content of mitochondrial complex I, based on flavin fluorescence scanning of a polyacrylamide gel after separation of complexes by Clear Native electrophoresis. By using mouse primary astrocytes as an example, an average value of 2.2 x 10(5) complex I molecules/cell was calculated.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Belem Yoval-Sanchez, Fariha Ansari, Joel James, Zoya Niatsetskaya, Sergey Sosunov, Peter Filipenko, Irina G. Tikhonova, Vadim Ten, Ilka Wittig, Ruslan Rafikov, Alexander Galkin
Summary: Pathologies associated with tissue ischemia/reperfusion (I/R) in highly metabolizing organs such as the brain and heart are leading causes of death and disability in humans. The molecular mechanisms underlying mitochondrial dysfunction during acute injury in I/R are tissue-specific, but their details are not completely understood. In this study, the researchers investigated the process of redox-dependent dissociation of flavin mononucleotide (FMN) from mitochondrial complex I in brain and heart mitochondria. They found that the presence of a brain-specific long 50 kDa isoform of the NDUFV3 subunit of complex I is responsible for the different kinetics of FMN loss in the brain compared to the heart. This study provides insights into the distinct molecular mechanism of I/R-induced mitochondrial impairment in cardiac and cerebral tissues.
Article
Biochemistry & Molecular Biology
Naybi Munoz-Cazares, Israel Castillo-Juarez, Rodolfo Garcia-Contreras, Victor Alberto Castro-Torres, Miguel Diaz-Guerrero, Jose S. Rodriguez-Zavala, Hector Quezada, Bertha Gonzalez-Pedrajo, Mariano Martinez-Vazquez
Summary: This study assessed the inhibition of quorum sensing (QS) and type III secretion systems (T3SSs) on Pseudomonas aeruginosa virulence. The synthesized halogenated furanones, named GBr, effectively reduced pyocyanin production, biofilm formation, and swarming motility, and inhibited the secretion of ExoS, ExoT, or ExoU effectors in tested strains. In a mouse model, GBr significantly decreased necrosis formation and bacterial spread more efficiently than C-30. Molecular docking analysis suggested that GBr had increased affinity for the active site of the QS LasR regulator. These findings highlight the potential of GBr as a multi-target anti-virulence therapy.
Article
Biochemistry & Molecular Biology
Abraham Josue Nevarez-Ramirez, Ana Laura Guzman-Ortiz, Pedro Cortes-Reynosa, Eduardo Perez-Salazar, Gustavo Alberto Jaimes-Ortega, Ricardo Valle-Rios, Alvaro Marin-Hernandez, Jose S. Rodriguez-Zavala, Eliel Ruiz-May, Jose Luis Castrejon-Flores, Hector Quezada
Summary: Cellular interactions within the bone marrow microenvironment can modulate leukemic cells and lead to drug resistance. In this study, intercellular protein transfer from mouse bone marrow stromal cells to human T-lymphoblasts was detected using shotgun proteomics. The transferred proteins were found to contribute to drug resistance in the leukemic cells. Shotgun proteomic analysis of co-cultured cells from different species could serve as a simple option for studying the proteins exchanged among interacting cells.
Article
Biochemistry & Molecular Biology
Belem Yoval-Sanchez, Luis Francisco Calleja, Maria de la Luz Hernandez-Esquivel, Jose Salud Rodriguez-Zavala
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2020)