Article
Biochemistry & Molecular Biology
Qiuling Tang, Xuchao Li, Yanping He, Kangsheng Ma
Summary: The study reveals the significant role of the CPR gene in insecticide resistance in Aphis gossypii. The expression of AgCPR is influenced by the developmental stage and concentration of insecticide, and silencing of AgCPR increases susceptibility to the insecticide, indicating its functional importance.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yichao Zhang, Hongli Li, Shaohang Yuan, Haihua Wu, Xiaojian Liu, Jianzhen Zhang
Summary: This study found that NADPH-cytochrome P450 reductase (CPR) in Locusta migratoria is involved in the activation of Precocene I. Experimental results showed that LmCPR interacts with NADPH, FAD, and FMN through hydrogen bonds. Treatment with Precocene I reduced the juvenile hormone levels in nymphs and prolonged the interval between fourth and fifth instar nymphs. Knockdown of LmCPR gene weakened the response to Precocene I, leading to a decrease in the occurrence rate of precocious adults induced by Precocene I treatment.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Christiane Ott, Florencia Tomasina, Nicolas Campolo, Silvina Bartesaghi, Mauricio Mastrogiovanni, Alejandro Leyva, Carlos Batthy, Walter Meinl, Tilman Grune, Rafael Radi
Summary: The proteasome has a decreased capability to cleave at C-terminal of NO2Y residues in proteins, leading to a possible factor that decreases the turnover of oxidized proteins and facilitates the accumulation of nitrated proteins.
Article
Biochemistry & Molecular Biology
Minrong He, Xueyi Zhao, Xingyu Chen, Yiyan Shi, Shuai Wu, Fujin Xia, Rongyu Li, Ming Li, Hu Wan, Jianhong Li, Xun Liao
Summary: The study found that Nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome P450 reductase (CPR) plays a crucial role in Nilaparvata lugens and its overexpression contributes to resistance to the insecticide sulfoxaflor as well as cross-resistance to other insecticides.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Shotaro Uehara, Yuichi Iida, Miyuki Ida-Tanaka, Motohito Goto, Kenji Kawai, Masafumi Yamamoto, Yuichiro Higuchi, Satoshi Ito, Riichi Takahashi, Hidetaka Kamimura, Mamoru Ito, Hiroshi Yamazaki, Mitsuo Oshimura, Yasuhiro Kazuki, Hiroshi Suemizu
Summary: This study developed a novel humanized liver mouse model that can accurately evaluate human drug metabolism without interference from mouse hepatocytes.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Physical
Michael J. Martinez, Jessica D. Carder, Evan L. Taylor, Eric P. Jacobo, ChulHee Kang, J. A. Brozik
Summary: This study measured the thermodynamic driving forces responsible for how strongly CPR partitions into a biomimetic ER with the same lipid composition. Results showed that the redox states of CPR have a significant impact on these driving forces.
JOURNAL OF PHYSICAL CHEMISTRY B
(2022)
Article
Biochemistry & Molecular Biology
Freeborn Rwere, Sangchoul Im, Lucy Waskell
Summary: By altering the 140s FMN binding loop of CYPOR, the activity with cyt P450s can be affected, with the mutant activity primarily determined by its ability to reduce ferric cyt P450. The different behaviors of the mutants are mainly due to the altered structure and mobility of the loop.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Entomology
Jian-Wen Qiao, Yong-Liang Fan, Bing-Jin Wu, Tian-Tian Bai, Ying-Hao Wang, Zhan-Feng Zhang, Dun Wang, Tong-Xian Liu
Summary: The study demonstrates that Acyrthosiphon pisum CPR modulates cuticular hydrocarbon (CHC) production, affecting its tolerance to desiccation stress and susceptibility to insecticides. This finding provides a novel target for pest control.
Article
Agronomy
Chen-Yang Yuan, Tian-Xing Jing, Wei Li, Xiao-Qiang Liu, Tian-Yuan Liu, Yi Liu, Meng-Ling Chen, Rui-Xu Jiang, Guo-Rui Yuan, Wei Dou, Jin-Jun Wang
Summary: The study cloned and sequenced the CPR gene of Asian citrus psyllid (D. citri) and found that the expression level of DcCPR was highest in the midgut and in nymphs. Knockdown of DcCPR increased the susceptibility of D. citri to imidacloprid and thiamethoxam, with a significant reduction in both DcCPR protein and P450s' specific activities. These results suggest that DcCPR is crucial for D. citri susceptibility to imidacloprid and thiamethoxam.
PEST MANAGEMENT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Li Shi, Wenlin Li, Yating Dong, Yao Shi, Yuliang Zhou, Xiaolan Liao
Summary: This study identified two transcripts of Spodoptera litura CPR (SlCPR-X1 and SlCPR-X2) and found that SlCPR-X1 plays a major role in indoxacarb resistance in S. litura. The study highlights the importance of insect CPR in xenobiotics detoxification and resistance development, suggesting CPR as a potential target for insecticide resistance management through RNAi or CRISPR/Cas.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Tamara Heintze, Denise Wilhelm, Thierry Schmidlin, Ute Hofmann, Ulrich M. Zanger, Matthias Schwab, Kathrin Klein
Summary: The study systematically investigated the impact of POR knockdown on a human hepatic cell model, revealing its widespread role in various metabolic pathways including drug metabolism and endogenous substance synthesis. POR disruption resulted in deregulated bile acid and cholesterol biosynthesis, as well as downregulation of key transcriptional regulators. Additionally, global protein expression was affected, with metabolic pathways and lipid metabolism being downregulated. The study provides valuable insights into the effects of diminished POR expression on drug and endogenous metabolism.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Narayanan Srividya, Iris Lange, Jana K. Richter, Matthias Wuest, B. Markus Lange
Summary: In this study, the enantioselectivity of multiple enzymes involved in monoterpenoid biosynthesis in peppermint and Japanese catnip was investigated. The experimental results showed that these enzymes exhibited different enantioselectivity towards intermediates of the pathway, and molecular modeling studies supported the experimental data. This study lays the groundwork for further structure-function studies on the origin of enantioselectivity in closely related species of the Lamiaceae.
Article
Biochemistry & Molecular Biology
Manuel Sellner, Andre Fischer, Charleen G. Don, Martin Smiesko
Summary: Research using molecular dynamics simulations and protein-protein docking revealed the mechanism of conformational changes between cytochrome P450 enzymes and cytochrome P450 reductase, as well as proposed new conformational transition mechanisms and structural mechanisms for susceptibility to redox states.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Xian Zou, Yue Zhang, Xu Zeng, Tuo Liu, Gui Li, Yuxin Dai, Yuanzhu Xie, Zhiyong Luo
Summary: In this study, two CPR genes (PgCPR1, PgCPR2) were isolated from ginseng adventitious roots, and both were found to be associated with the biosynthesis of ginsenoside. These genes showed higher expression in stem and main root tissues of Panax ginseng, and their expression levels were induced after MeJA treatment, correlating with ginsenoside accumulation.
Article
Chemistry, Physical
Nico D. Fessner, Christopher Grimm, Matic Srdic, Hansjoerg Weber, Wolfgang Kroutil, Ulrich Schwaneberg, Anton Glieder
Summary: This study explores the synthetic potential of human P450 3A4 in diversifying natural product classes, resulting in the identification of 31 authentic human metabolites. With efficient expression levels in P. pastoris, this biocatalyst shows promising results for modifying natural products in a one-step fashion.
Article
Chemistry, Medicinal
Jaime D'Agostino, Haoming Zhang, Cesar Kenaan, Paul F. Hollenberg
CHEMICAL RESEARCH IN TOXICOLOGY
(2015)
Article
Biochemical Research Methods
Yoshihiro Morishima, Haoming Zhang, Miranda Lau, Yoichi Osawa
ANALYTICAL BIOCHEMISTRY
(2016)
Article
Pharmacology & Pharmacy
Hsia-lien Lin, Haoming Zhang, Paul F. Hollenberg
DRUG METABOLISM AND DISPOSITION
(2018)
Article
Biochemistry & Molecular Biology
Haoming Zhang, Adam L. Yokom, Shen Cheng, Min Su, Paul F. Hollenberg, Daniel R. Southworth, Yoichi Osawa
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Min Su, Sumita Chakraborty, Yoichi Osawa, Haoming Zhang
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Review
Biochemistry & Molecular Biology
Yudong Sun, Xiaoqiang Huang, Yoichi Osawa, Yuqing Eugene Chen, Haoming Zhang
Summary: This article highlights the unique features of wild-type cytochrome P450 CYP102A1 from Bacillus megaterium as a highly efficient monooxygenase, as well as the significant progress made in engineering CYP102A1 for various applications. The repertoire of engineered CYP102A1 variants continues to expand, covering a wide range of substrates. The objective of the article is to provide a concise review of the latest advancements, with reference to related literature on CYP102A1.
Article
Biochemistry & Molecular Biology
Shen Cheng, Zhiyuan Bo, Paul Hollenberg, Yoichi Osawa, Haoming Zhang
Summary: The study reports the use of amphipols to form a fully functional, soluble, homogeneous preparation of full-length CYP:POR complexes for biochemical and structural study. The stability and structure of the complex were validated using negative stain electron microscopy, revealing a tetrameric complex embedded in one amphipol nanoparticle. This represents the first report of the full-length mammalian CYP:POR complex by transmission EM and highlights the potential use of amphipols in biochemical and structural studies of functional CYP complexes.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Gastroenterology & Hepatology
Dhiman Maitra, Eric L. Carter, Rani Richardson, Laure Rittie, Venkatesha Basrur, Haoming Zhang, Alexey Nesvizhskii, Yoichi Osawa, Matthew W. Wolf, Stephen W. Ragsdale, Nicolai Lehnert, Harald Herrmann, M. Bishr Omary
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2019)
Article
Pharmacology & Pharmacy
Dale A. Lauver, Dawn S. Kuszynski, Barbara D. Christian, Matthew P. Bernard, James P. Teuber, Bruce E. Markham, Yuqing E. Chen, Haoming Zhang
PHARMACOLOGY RESEARCH & PERSPECTIVES
(2019)
Article
Pharmacology & Pharmacy
Hsia-lien Lin, Haoming Zhang, Vyvyca J. Walker, Jaime D'Agostino, Paul F. Hollenberg
DRUG METABOLISM AND DISPOSITION
(2017)
Article
Pharmacology & Pharmacy
Georgina C. B. Nicholl, Ali K. Jawad, Robert Weymouth, Haoming Zhang, Asim A. Beg
BRITISH JOURNAL OF PHARMACOLOGY
(2017)
Article
Pharmacology & Pharmacy
Hsia-lien Lin, Haoming Zhang, Cesar Kenaan, Paul F. Hollenberg
DRUG METABOLISM AND DISPOSITION
(2016)
Article
Pharmacology & Pharmacy
Haoming Zhang, D. Adam Lauver, Hui Wang, Duxin Sun, Paul F. Hollenberg, Y. Eugene Chen, Yoichi Osawa, Daniel T. Eitzman
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2016)
Article
Pharmacology & Pharmacy
Diane M. Calinski, Haoming Zhang, Susan Ludeman, M. Eileen Dolan, Paul F. Hollenberg
DRUG METABOLISM AND DISPOSITION
(2015)
Meeting Abstract
Chemistry, Multidisciplinary
Haoming Zhang, Paul F. Hollenberg
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2014)