ENZYMATIC IN-SITU GENERATION OF H2O2 FOR DECOLORIZATION OF ACID BLUE 113 BY FENTON PROCESS

Decolorization of Acid Blue 113 in an aqueous medium by bio-Fenton process has been investigated in this study. Enzymatic oxidation of glucose was performed for in-situ generation of H2O2, which was employed to react with Fe2+ for producing hydroxyl radicals. The effects of various parameters, including concentrations of AB 113, glucose, and FeSO4, activity of glucose oxidase (GOx) and pH were assessed. The highest decolorization of AB 113 was achieved at Fe2+ concentration of 0.2 mmol/L, pH 4.0, glucose concentration of 0.018 mol/L, and glucose oxidase activity of 2500 U/L in the constant temperature (23 +/- 0.1 degrees C) and constant shaking rate (160 r/min), while the concentration of AB 113 was 40 mg/L. Under these conditions, the obtained AB 113 decolorization efficiency after 60 min was about 95%.