Article
Biotechnology & Applied Microbiology
Jing Zhang, Yue Yuan, Zhiwen Wang, Tao Chen
Summary: In this study, Halomonas bluephagenesis was engineered to efficiently produce Mevalonate (MVA). By screening MVA synthetases and introducing beneficial genes, along with the utilization of mixed carbon sources and non-oxidative glycolysis pathway, the MVA titer and yield were significantly improved. This study highlights the potential of H. bluephagenesis as a chassis for MVA production.
METABOLIC ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Sean F. Sullivan, Anuj Shetty, Tharun Bharadwaj, Naveen Krishna, Vikas D. Trivedi, Venkatesh Endalur Gopinarayanan, Todd C. Chappell, Daniel M. Sellers, R. Pravin Kumar, Nikhil U. Nair
Summary: Engineering the utilization of non-native substrates in industrial microbes provides a way to use renewable sources for bioprocesses. Previous research has shown that activating the GAL regulon in Saccharomyces cerevisiae during growth on the non-native substrate xylose enhances gene expression and growth compared to constitutive overexpression of the same pathway. However, this method was not easily adaptable to other non-native substrates. In this study, a variant of Gal3p called Gal3pMC was constructed, which can activate the GAL regulon with diverse substrates and mimic the dynamics of the native system. Testing this regulon approach with non-native lignocellulosic sugars resulted in higher growth rates and final cell densities compared to constitutive overexpression. The co-utilization of multiple non-native substrates suggests that this regulon approach could be universally beneficial for engineering synthetic heterotrophy.
METABOLIC ENGINEERING
(2023)
Review
Biochemistry & Molecular Biology
Christopher D. Reinkemeier, Edward A. Lemke
Summary: Eukaryotic cells compartmentalize specific functions into specialized organelles, with dynamic biomolecular condensates offering opportunities for synthetic biology. Multiple orthogonally translating organelles have been designed to enable precise protein engineering inside living cells, creating mammalian cells with multiple expanded genetic codes. This provides a pathway to engineer multiple proteins with multiple and distinct functionalities inside living eukaryotes and offers a general strategy toward spatially orthogonal enzyme engineering.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ana P. Teixeira, Pascal Stucheli, Simon Auslander, David Auslander, Pascal Schonenberger, Samuel Hurlemann, Martin Fussenegger
Summary: The researchers have developed an antibiotic-free selection system for generating stable transgenic cell lines, which utilizes cellobiose as the primary energy source. By combining this system with a PiggyBac transposase-based integration strategy, they were able to efficiently generate stable transgenic cell lines that expressed high levels of cargo proteins. This strategy was successfully validated by integrating sequences encoding two biopharmaceutical proteins, erythropoietin and the monoclonal antibody rituximab, and confirmed their efficient production in both cellobiose- and glucose-containing medium.
METABOLIC ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Irene Otero-Muras, Pablo Carbonell
Summary: Metabolic engineering involves optimizing processes from single-cell to fermentation to increase production of valuable chemicals. A systems approach has accelerated scaling from rapid prototyping to industrial production, with automated DNA assembly reducing time from conception to production. The success of metabolic engineering often relies on retrobiosynthetic protocols and dynamic regulation strategies assembled as genetic circuits in host strains.
METABOLIC ENGINEERING
(2021)
Review
Biochemistry & Molecular Biology
Dongsoo Yang, Hyunmin Eun, Cindy Pricilia Surya Prabowo
Summary: Polyketides, a type of natural products, have versatile applications such as pharmaceuticals, nutraceuticals, and cosmetics. Aromatic polyketides, including type II and III polyketides, are important chemicals for human health, such as antibiotics and anticancer agents. However, they are mainly produced from challenging sources like soil bacteria or plants. To address this issue, metabolic engineering and synthetic biology have been used to efficiently engineer model microorganisms for enhanced production of aromatic polyketides. This review discusses the recent advancements and future prospects of metabolic engineering and synthetic biology strategies in the production of type II and type III polyketides in model microorganisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Yu Zhou, Evan N. Mirts, Sangdo Yook, Matthew Waugh, Rachel Martini, Yong-Su Jin, Yi Lu
Summary: We report a biocatalytic system for direct generation of TAL derivatives under mild conditions by rationally engineering the 2PS active site and rewiring the biocatalytic pathway in the metabolic network of E. coli. The system can produce high-value products, such as kavalactone precursors, with yields up to 17 mg/L culture. Computer modeling suggests that sterics and hydrogen-bond interactions play key roles in tuning the selectivity, efficiency, and yield.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Biotechnology & Applied Microbiology
Kim-Ngan T. Tran, Ashokkumar Kumaravel, Soon Ho Hong
Summary: Metabolic engineering has become an attractive choice for efficient bio-refinery processes or biochemical production. However, engineered metabolic pathways often suffer from flux imbalances due to a lack of corresponding regulatory mechanisms. Protein scaffolds have been created to co-localize proteins and enhance metabolic productivity.
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Yan Guo, Fuxiao Li, Jingyi Zhao, Xin Wei, Zhiqi Wang, Jidong Liu
Summary: This study successfully increased the production of Geraniol in engineered yeast strains by modifying the mevalonate pathway. By introducing an isopentenyl phosphate kinase and establishing an isopentenyl phosphate utilization bypass, optimizing key rate-limiting enzymes, and using molasses as a carbon source, the production of Geraniol was significantly enhanced.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Review
Biotechnology & Applied Microbiology
Govinda R. Navale, Mahesh S. Dharne, Sandip S. Shinde
Summary: Isoprenoids, also known as terpenoids, are diverse organic compounds found abundantly in nature, with various applications in pharmaceutical, nutraceutical, and chemical industries. Recent advancements in metabolic engineering have enabled the production of isoprenoids in heterologous host systems like Escherichia coli and Saccharomyces cerevisiae. Challenges and strategies for scale-up and engineering of isoprenoids in heterologous host systems have been discussed in detail.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Christina Jonsson, Ren Wei, Antonino Biundo, Johan Landberg, Lisa Schwarz Bour, Fabio Pezzotti, Andreea Toca, Les M. Jacques, Uwe T. Bornscheuer, Per-Olof Syren
Summary: Although the separation of individual components from polymer blends in textiles is currently unavailable at a viable scale, biotechnology may provide a solution. However, biotechnological-assisted depolymerization only works for a few synthetic polymer chains, and the use of difficult-to-recycle blended materials is rapidly growing in the textile industry.
Review
Chemistry, Multidisciplinary
Samuel J. Cobb, Santiago Rodriguez-Jimenez, Erwin Reisner
Summary: Electrocatalytic CO2 reduction is a diverse field with different types of catalysts. Enzymes and small molecule catalysts have distinct active sites, while heterogeneous materials have complex and poorly understood active sites. However, as the performance of active sites improves, the gap between these catalysts is closing, enabling the use of different types of catalysts without barriers.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Editorial Material
Multidisciplinary Sciences
Alison Abbott
Summary: Researchers are interested in understanding the reasons behind cell secretion of vesicles and the functions of these vesicles during delivery.
Review
Plant Sciences
Revuru Bharadwaj, Sarma R. Kumar, Ashutosh Sharma, Ramalingam Sathishkumar
Summary: Gene clusters responsible for specialized metabolites in plants offer a potential untapped source for highly useful biomolecules. These clusters evolve through gene duplications and neofunctionalization, tightly regulated at nucleosome level. Further research is needed to explore the significance and regulatory mechanisms of these gene clusters in plant biosynthesis.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Plant Sciences
Revuru Bharadwaj, Sarma R. Kumar, Ashutosh Sharma, Ramalingam Sathishkumar
Summary: Plants contain gene clusters encoding enzymes for the biosynthesis of specialized metabolites, which have evolved through gene duplications and neofunctionalization and are tightly regulated at nucleosome level. The prevalence of these gene clusters presents an attractive possibility of untapped source of highly useful biomolecules in plants.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemical Research Methods
Kai Deng, Xi Wang, Nicole Ing, Paul Opgenorth, Markus de Raad, Jinho Kim, Blake A. Simmons, Paul D. Adams, Anup K. Singh, Taek Soon Lee, Trent R. Northen
Summary: We developed a novel mass spectrometry-based assay that allows rapid quantification of primary alcohols produced by cell cultures. This assay combines TEMPO-based oxidation chemistry and oxime ligation, followed by product analysis using Nanostructure-Initiator Mass Spectrometry. It can accurately monitor C5 to C18 alcohols, as well as glucose and gluconate in the growth medium, which is crucial for strain characterization and optimization. The assay shows comparable results to gas chromatography for isoprenol production but requires significantly less acquisition time per sample. By applying this assay, we gained new insights into the utilization of alcohols by P. Putida and found that this strain had limited growth on heptanol and octanol.
ANALYTICAL BIOCHEMISTRY
(2023)
Review
Plant Sciences
Xu Lu, Lijin Huang, Henrik Scheller, Jay D. Keasling
Summary: Terpenoid glycosides derived from medicinal plants have shown significant curative effects in the treatment of various diseases. UDP-dependent glycosyltransferases in plants play a crucial role in the biosynthesis of bioactive glycosides. In this review, the characteristics, evolution, and recent advances in terpenoid UDP-glycosyltransferases (UGTs) in medicinal plants over the past 20 years are summarized, providing a reference for the study of glycosylation and biosynthetic pathways of medicinal terpenoids in plants.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Chemistry, Physical
Brandon J. Bloomer, Sean N. Natoli, Marc Garcia-Borras, Jose H. Pereira, Derek B. B. Hu, Paul D. Adams, K. N. Houk, Douglas S. Clark, John F. Hartwig
Summary: Artificial metalloenzymes (ArMs), containing synthetic metal cofactors, are widely used as biocatalysts for unnatural reactions. However, detailed mechanistic information on the effects of protein scaffold on ArM structure and reactivity is limited. This study presents the structure of an unnatural P450 analogue and kinetic analysis of its reaction, revealing a mechanism initiated by cofactor conformational change and highlighting the influence of cofactor dynamics on the catalytic reaction of ArMs.
Article
Chemistry, Multidisciplinary
Elias Englund, Matthias Schmidt, Alberto A. Nava, Anna Lechner, Kai Deng, Renee Jocic, Yingxin Lin, Jacob Roberts, Veronica T. Benites, Ramu Kakumanu, Jennifer W. Gin, Yan Chen, Yuzhong Liu, Christopher J. Petzold, Edward E. K. Baidoo, Trent R. Northen, Paul D. Adams, Leonard Katz, Satoshi Yuzawa, Jay D. Keasling
Summary: This study successfully altered the extension substrates of modular polyketide synthases (PKSs) by exchanging acyltransferase (AT) domains, resulting in the synthesis of 13 structurally different polyketides in vitro. The results greatly enhance our understanding of rare AT domains and demonstrate the benefit of using PKS engineering strategy to produce novel chemicals in vitro.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Multidisciplinary Sciences
Jing Huang, Andrew Quest, Pablo Cruz-Morales, Kai Deng, Jose Henrique Pereira, Devon Van Cura, Ramu Kakumanu, Edward E. K. Baidoo, Qingyun Dan, Yan Chen, Christopher J. Petzold, Trent R. Northen, Paul D. Adams, Douglas S. Clark, Emily P. Balskus, John F. Hartwig, Aindrila Mukhopadhyay, Jay D. Keasling
Summary: Biosynthesis is a renewable approach for producing natural and new-to-nature products, but it has a narrower scope compared to synthetic chemistry. Recent research has shown that carbene-transfer reactions can be performed in cells, but exogenous carbene donors and unnatural cofactors are needed, limiting the scalability of the biosynthesis process. This study presents a microbial platform for intracellularly conducting abiological carbene-transfer reactions, expanding the range of organic products that can be produced. Evaluation: 8 out of 10.
Article
Biochemical Research Methods
Kevin Yin, Pablo Cruz-Morales, Christopher M. Whitford, Jay D. Keasling
Summary: Polycyclopropanated (POP) compounds have the potential to be a more energy-dense alternative to current jet and rocket fuels, but further development is needed. This protocol outlines the production of polycyclopropanated fatty acids in Streptomyces, though the production of POP in other hosts has yet to be demonstrated. This method serves as a baseline for the future development of POP and other polyketide products. For more information, please refer to Cruz-Morales et al. (2022).
Article
Biotechnology & Applied Microbiology
Shilva Shrestha, Deepika Awasthi, Yan Chen, Jennifer Gin, Christopher J. Petzold, Paul D. Adams, Blake A. Simmons, Steven W. Singer
Summary: This study investigated the carbon catabolite repression (CCR) functioning in P. putida M2, a strain capable of consuming sugars and aromatic compounds. The results showed that incomplete substrate consumption occurred during cultivation with a mixture of glucose and aromatic compounds, while transient intermediate accumulation and complete aromatic consumption were observed during xylose-aromatic consumption. Proteomics analysis revealed stronger repression of aromatic catabolic proteins by glucose than xylose. Additionally, CRISPRi-based gene repression experiments showed that reduction of crc expression led to faster growth and improved glucose and p-coumarate uptake. Small RNA sequencing results indicated lower levels of CrcY and CrcZ homologues under strong CCR conditions.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Taichi E. Takasuka, Hoon Kim, Kai Deng, Christopher M. Bianchetti, Kaho Yamashita, Emily T. Beebe, Lai F. Bergeman, Kirk A. Vander Meulen, Samuel Deutsch, John Ralph, Paul D. Adams, Trent R. Northen, Brian G. Fox
Summary: This study demonstrates that a combination of a broad-specificity β-1,3-exoglucanase and a polysaccharide lyase from family 18 can efficiently hydrolyze untreated kelp into soluble sugars, such as glucose, gentiobiose, mannitol-end glucose, and mannuronic and guluronic acids and their soluble oligomers. The data suggest that enzyme combinations targeted to the unique polysaccharide composition of marine biomass are sufficient to deconstruct kelp into soluble sugars for microbial fermentation.
Article
Multidisciplinary Sciences
Xiaoyue Chen, Graham A. Hudson, Charlotte Mineo, Bashar Amer, Edward E. K. Baidoo, Samantha A. Crowe, Yuzhong Liu, Jay D. Keasling, Henrik V. Scheller
Summary: Methyl jasmonate (MeJA) is a known elicitor of plant specialized metabolism, including triterpenoid saponins. In this study, we leveraged MeJA-induced saponin biosynthesis in S. vaccaria and identified multiple enzymes that catalyze the oxidation and glycosylation of triterpenoids. We also discovered the biosynthetic pathway for the rare nucleotide sugar UDP-d-fucose. Our findings enable the production and optimization of high-value saponins in microorganisms and plants through synthetic biology approaches.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Lucas Waldburger, Mitchell G. Thompson, Alexandra J. Weisberg, Namil Lee, Jeff H. H. Chang, Jay D. Keasling, Patrick M. M. Shih
Summary: Transcription start sites (TSSs) play a fundamental role in understanding gene expression and regulation. Agrobacteria are prokaryotes widely used in plant biotechnology due to their ability to transfer DNA into host plant genomes. However, the genome-wide transcriptional regulation of agrobacteria, especially in less-studied lineages, remains poorly understood. A study using differential RNA-seq and an optimized algorithm has successfully identified thousands of TSSs with nucleotide resolution for representatives of each lineage, providing a framework for understanding the mechanistic basis and evolution of pathology across the three main lineages of agrobacteria. The importance of parameter optimization in genome-wide TSS identification and genomics is also highlighted by the optimized algorithm.
Article
Food Science & Technology
Vayu Maini Rekdal, Nabila Rodriguez-Valeron, Mikel Olaizola Garcia, Diego Prado Vasquez, Pia M. Sorensen, Rasmus Munk, Jay D. Keasling
Summary: This study explores novel culinary applications of Neurospora intermedia, an edible fungus traditionally used in Java, Indonesia. The fungus can be used to produce oncom-like meat alternatives and enzymes for starch-to-sugar conversion. It also adds orange color and distinct volatile aroma to dishes. The novel foods produced in this study received favorable ratings in consumer trials.
INTERNATIONAL JOURNAL OF GASTRONOMY AND FOOD SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Aidan E. Cowan, Sarah H. Klass, Peter H. Winegar, Jay D. Keasling
Summary: Anthropogenic carbon emissions are causing rapid climate change and posing risks to ecosystems and human society. Innovations in microbial fermentation enable the production of fuels, chemicals, and materials without the use of fossil resources, thus reducing carbon emissions. Recent advances in microbial fermentation aim to increase product yield and efficiency, while also lowering production costs to compete economically with fossil-derived chemicals.
CURRENT OPINION IN SYSTEMS BIOLOGY
(2023)
Article
Biochemical Research Methods
Noel S. Ha, Jenny R. Onley, Kai Deng, Peter Andeer, Benjamin P. Bowen, Kshitiz Gupta, Peter W. Kim, Nathaniel Kuch, Mark Kutschke, Alex Parker, Fangchao Song, Brian Fox, Paul D. Adams, Markus de Raad, Trent R. Northen
Summary: Mass spectrometry combined with microfluidics in the Drop-NIMS platform enables rapid screening of enzymatic reactions and identification of different combinations of substrates and enzymes for various applications.
Article
Plant Sciences
Pradeep Kumar Prabhakar, Jose Henrique Pereira, Rahil Taujale, Wanchen Shao, Vivek S. Bharadwaj, Digantkumar Chapla, Jeong-Yeh Yang, Yannick J. Bomble, Kelley W. Moremen, Natarajan Kannan, Michal Hammel, Paul D. Adams, Henrik V. Scheller, Breeanna R. Urbanowicz
Summary: The researchers describe the structure of GalS1 from Populus trichocarpa, which consists of an N-terminal domain and a C-terminal catalytic domain. They propose a mechanism for GalS1 catalysis and a new model for pectin biosynthesis based on deep evolutionary analysis, molecular simulations, and biochemical studies.
Article
Biochemistry & Molecular Biology
Sung Cheon Ko, Han Min Woo
Summary: This study reports a method for bacterial RNA repression using the CRISPR-dCas13a system. By using programmable crRNAs, trans-acting and cis-acting sRNAs can be repressed, altering the regulatory mechanisms and stress-related phenotypes in bacteria. This system can be used for discovering novel sRNAs and fine-tuning bacterial RNA repression in both scientific and industrial applications.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Engineering, Chemical
Qing Han, Mengqing Shi, Linkai Han, Di Liu, Mingwei Tong, Yuxin Xie, Zhonghua Xiang
Summary: Developing highly efficient bifunctional oxygen electrocatalysts is crucial for zinc-air flow batteries. Metal-organic frameworks (MOFs) and covalent organic polymers (COPs) have emerged as promising alternatives due to their designable and controllable atomic-level structures. However, their catalytic performances are limited by conductivity and catalytic activity. In this study, nanosheet FeNi-MOF and iron phthalocyanine rich COP hybrid materials are assembled through the pi-pi stacking effect to create highly efficient bifunctional electrocatalysts. The resulting catalyst exhibits superior catalytic performance and stability, making it a promising candidate for zinc-air flow batteries.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Daria Grigorash, Dmytro Mihrin, Rene Wugt Larsen, Erling H. Stenby, Wei Yan
Summary: The article introduces a new approach to describe the cross-association between molecules, allowing for the simulation of weakly bound molecular complexes with different conformations in mixtures. By incorporating this approach into the equation of state, accurate predictions of vapor-liquid equilibrium and liquid-liquid equilibrium can be made. The new method is validated through experiments on alcohol and acid mixtures, with the results compared to experimental data, demonstrating its accuracy and reliability.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Mohammed Al-Sharabi, Daniel Markl, Vincenzino Vivacqua, Prince Bawuah, Natalie Maclean, Andrew P. E. York, Axel Zeitler
Summary: This study used terahertz pulsed imaging to investigate the transport process of different solvents into ceramic catalytic materials. The results showed that the heating rate of the samples influenced the water transport rate, while the viscosity of 1-octanol slowed down its transport.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Chukwunonso Anyaoku, Sati Bhattacharya, Rajarathinam Parthasarathy
Summary: This study aimed to enhance understanding of settling dynamics in viscoelastic fluids by developing a semi-empirical correlation and a dimensionless ratio, which accurately described the characteristics of settling suspensions.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Antti I. Koponen, Janika Viitala, Atsushi Tanaka, Baranivignesh Prakash, Olli-Ville Laukkanen, Ari Jasberg
Summary: This study focuses on the development of foam application chemicals for the paper and board industry. The research explores the rheology of the polyvinyl alcohol foam used in the process. Measurements were conducted to determine the foam viscosity and slip flow. The results suggest that slip flow contributes significantly to the total flow rate, and the obtained viscosity and slip models provide a solid foundation for industrial processes.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Dalei Sun, Jinghui Cai, Yating Yang, Zhiwu Liang
Summary: In this study, Fe-doped alpha-Bi2O3 catalysts with different Fe/Bi molar ratios were synthesized and utilized in the carbonylation of isobutyl amine with CO2. The results showed that Fe doping significantly enhanced the catalytic abilities of alpha-Bi2O3.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Yuan Tian, Xinxin Wang, Yanrong Liu, Wenping Hu
Summary: This paper predicts the solubility of nitrogen gas in ionic liquids (ILs) using two quantitative structure-property relationship (QSPR) models. By combining machine learning methods and ionic fragments contribution method, the accuracy and reliability of the prediction models are improved.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Liwang Wang, Wei Liu, Pan Yang, Yulong Chang, Xiaoxu Duan, Lingyu Xiao, Yaoming Hu, Jiwei Wu, Liang Ma, Hualin Wang
Summary: This study investigates the effective phase interfacial area (ae) of hydro-jet cyclones at different injection angles. The results show that a 45 degrees upward incidence angle yields the most favorable flow field characteristics for efficient mass transfer. The significant enhancement in ae of the hydro-jet cyclones offers the advantage of reducing equipment volume and cost savings.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Chuanjun Wu, Jiangzhi Chen, Jiyue Sun, I-Ming Chou, Shenghua Mei, Juezhi Lin, Lei Jiang
Summary: In this study, the solubility of H2S hydrate in water was measured using Raman spectroscopy. The results showed that the solubility increases with temperature under certain equilibrium conditions, and the solubility also depends on pressure and temperature under different equilibrium conditions. A thermodynamic model based on the van der Waals-Platteeuw theory was developed to predict the solubility, demonstrating its accuracy.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Lorenzo Brivio, Serena Meini, Mattia Sponchioni, Davide Moscatelli
Summary: This study investigates the influence of three main parameters and proposes a kinetic model to predict the optimal operating conditions for high yield of dimethyl terephthalate (DMT) in the chemical recycling process of polyethylene terephthalate (PET).
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Hongju Lin, Fanhui Liao, Yanchang Chu, Mingyu Xie, Lun Pan, Yuanyuan Wang, Lijian Leng, Donghai Xu, Le Yang, Gangfeng Ouyang
Summary: A honeycomb NiCo/C-Na catalyst with a micro-meso-macroporous structure has been fabricated and shown to have significantly higher catalytic activity for the decarboxylation of fatty acids. It also proves to be efficient in upgrading sludge HTL bio-crude, resulting in a biofuel with decreased viscosity and increased density.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Xiaoxian Li, Rui Li, Min Lin, Mingde Yang, Yulong Wu
Summary: A series of coated non-noble metal porous carbon catalysts were synthesized and applied to the aqueous-phase deoxygenation of algal bio-oil. One of the catalysts showed excellent deoxygenation selectivity and catalytic activity at 250 degrees C. The catalyst exhibited good hydrothermal stability and the reaction mechanism was proposed based on product analysis and active site analysis.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
M. V. Chudakova, M. V. Popov, P. A. Korovchenko, E. O. Pentsak, A. R. Latypova, P. B. Kurmashov, A. A. Pimenov, E. A. Tsilimbaeva, I. S. Levin, A. G. Bannov, A. V. Kleymenov
Summary: A series of catalysts with different potassium contents were prepared using solution combustion synthesis and characterized using various techniques. The results showed that the potassium content affected the phase composition and texture of the catalysts. The addition of a small amount of potassium resulted in a change in particle size distribution, leading to higher hydrogen yield. The Ni-1%K2O/Al2O3 catalyst exhibited the highest hydrogen yield at temperatures of 675 and 750 degrees Celsius.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Aliakbar Roosta, Nima Rezaei
Summary: In this study, we modified the electrolyte cubic plus association equation of state (e-CPA EoS) and integrated it with two electrical conductivity models to estimate the electrical conductivity of 11 monovalent electrolyte solutions in water. The modified e-CPA model demonstrated better performance and the hybridization with electrical conductivity models resulted in two predictive models for estimating the electrical conduction of dilute and concentrated electrolyte solutions. These predictive models showed relative average percentage deviations (AARD) of 11.15% and 13.87% over wide ranges of temperature and electrolyte concentration.
CHEMICAL ENGINEERING SCIENCE
(2024)
Article
Engineering, Chemical
Haoren Niu, Jianzheng Wang, Qingzhu Jia, Qiang Wang, Jin Zhao, Fangyou Yan
Summary: A study developed two quantitative structure-property relationship models for the complexation performance of alpha- and beta-cyclodextrins and validated their stability and predictive ability through internal and external validation. The models showed robustness and satisfactory performance, as demonstrated by the experimental results and model validations. These models can effectively predict the binding constants between cyclodextrins and various types of molecules, providing valuable tools for cyclodextrin design.
CHEMICAL ENGINEERING SCIENCE
(2024)