Article
Chemistry, Physical
Ke Jiang, Li Li, Shuxin Liu, Yudong Xu, Jiayao Yang, Wanying Chu, Ping Lu, Ting Gao, Ruoxuan Bai, Fangxu Xu, Hongxin Zhao
Summary: In this study, mutants of E. aerogenes were constructed using different strategies to enhance hydrogen production by regulating NADH supply. The hydrogen yields of the mutants IAM1183-E, IAM1183-L, and IAM1183-EL increased by 23.3%, 81.7%, and 97.9%, respectively, compared to the wildtype strain. Overexpression of the pncB gene also led to increased hydrogen yields in the mutants. The strain IAM1183-EL/P exhibited the highest production efficiency with a 58% higher total hydrogen yield than the wildtype. Metabolite analysis revealed that the mutants with nuoE and ldhA gene deletions, combined with pncB overexpression, resulted in a redistribution of metabolic fluxes, leading to improved hydrogen yield.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Biotechnology & Applied Microbiology
Tomoya Maeda, Abigail Koch-Koerfges, Michael Bott
Summary: The oxidation of NADH and reduction of quinone is crucial for cellular metabolism. In this study with Corynebacterium glutamicum, Ndh was found to be the main enzyme for NADH oxidation, while Mdh and LdhA can functionally replace Ndh. The study also showed that Mdh and LdhA, along with Mqo and LldD, play active roles in NADH oxidation and menaquinone reduction.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Physical
Ruoxuan Bai, Wanying Chu, Zimu Qiao, Ping Lu, Ke Jiang, Yudong Xu, Jiayao Yang, Ting Gao, Fangxu Xu, Hongxin Zhao
Summary: This study aimed to improve hydrogen production in Enterobacter aerogenes IAM1183 by regulating NADH supply and metabolic flux via disruption of the nuoF gene, over-expression of formate hydrolyase activator (fhlA), and phosphoenolpyruvate carboxykinase (pckA). The results showed that knocking out nuoF and overexpressing pckA significantly increased the intracellular NADH/NAD+ ratio, leading to improved hydrogen production through the NADH pathway. Over-expression of fhlA also increased hydrogen production via the formate pathway. Through multi-gene engineering, the metabolic flux of mutants was reallocated, resulting in a higher total hydrogen production compared to the original strain.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Article
Biotechnology & Applied Microbiology
Ke Jiang, Ruoxuan Bai, Ting Gao, Ping Lu, Jingya Zhang, Shuting Zhang, Fangxu Xu, Shenghou Wang, Hongxin Zhao
Summary: In this study, the researchers altered the activity of Complex I by knocking out nuoE, nuoF, and nuoG genes and overexpressing the maeA gene to increase the production of hydrogen. The optimized strain IAM1183-EFG/M showed a 65% increase in hydrogen production compared to the original strain, with significant changes in metabolite content. In a bioreactor, the scale-up IAM1183-EFG/M strain achieved a total hydrogen production of 4.76 L after 44 hours of fermentation, an 18% increase compared to the starting strain. This study provides a new direction for microbial hydrogen production through combinational modification of multiple genes.
MICROBIAL CELL FACTORIES
(2023)
Article
Plant Sciences
Qiyu He, Jianfeng Jin, Pengfei Li, Huihui Zhu, Zhanqi Wang, Wei Fan, Jian Li Yang
Summary: The study identified and characterized the SlFDH gene in tomato and found that it plays a role in aluminum tolerance. The expression of SlFDH was induced by aluminum stress but not by other metals. CRISPR/Cas9-generated mutants lacking the SlFDH gene were more sensitive to aluminum stress and formate. The transcription factor SlSTOP1 was found to positively regulate SlFDH expression by directly binding to its promoter.
Article
Electrochemistry
C. Barrientos, R. Moscoso, S. Moris, J. A. Squella
Summary: In this study, three new nitroaromatic compounds were synthesized and electrochemically studied on nanostructured electrodic platforms. It was found that these compounds were easily reducible, generating stable redox mediator couples.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2021)
Article
Biochemistry & Molecular Biology
Silvia Yumnam, Min Cheol Kang, Seung Hyun Oh, Hak Cheol Kwon, Jin Chul Kim, Eun Sung Jung, Choong Hwan Lee, Ai-Young Lee, Jong-Ik Hwang, Sun Yeou Kim
Summary: Suppressing dihydrolipoyl dehydrogenase (DLD) increases intracellular ROS production, decreases mitochondrial membrane potential, induces autophagic cell death, and significantly inhibits melanoma growth and proliferation. Furthermore, DLD suppression alters energy metabolism in melanoma cells.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Sandra Zarska, Rafal Szukiewicz, Sergiu Coseri, Volodymyr Pavlyuk, Dorota Krasowska, Wojciech Ciesielski
Summary: This paper presents new selenium derivatives obtained by modifying the surface of carbon nanotubes. The synthesis was carried out in mild conditions, and the obtained products were characterized using various methods. The selenium derivatives of carbon nanotubes exhibited high selenium and phosphorus contents, reaching 14% and 4.2% respectively.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biophysics
Siyu Chen, Keshuai Shang, Xinyu Gao, Xia Wang
Summary: This study proposed a synergistic strategy based on the co-catalysis of NAD(+)-dependent dehydrogenases and nanoporous gold (NPG) for the oxidation of substrates and NADH. A biosensing platform was developed by modifying screen-printed electrode (SPE) with NPG and NAD(+)- dependent dehydrogenase for the electrochemical detections of NADH, ethanol, and glucose. The results showed that this biosensing platform exhibited good sensitivity and low detection limit for micro-sample detection, achieving comparable accuracy as automatic analyzers in real samples.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biotechnology & Applied Microbiology
Xue-Yong Li, Meng-Qiu Xu, Hui Liu, Qiang Zhou, Jian Gao, Ye-Wang Zhang
Summary: The combi-CLEAs, consisting of Gdh and Nox, showed an activity of 210 U g(-1) under optimized preparation conditions. They exhibited higher thermostability and retained 51.5% of their original activity after eight cycles of reuse. The combi-CLEAs were successfully used for the synthesis of l-tagatose without any by-products, showcasing their potential for industrial bioconversion of galactitol.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2022)
Article
Materials Science, Multidisciplinary
Zakaria Saadi, Simon G. King, Jose Anguita, Vlad Stolojan, S. Ravi P. Silva
Summary: Thermoelectric materials offer a promising solution for recovering wasted energy, but their efficiency is low and the best materials are brittle, toxic, and expensive. This study demonstrates that flexible films of double-walled carbon nanotubes can achieve low thermal conductivity and high electrical conductivity, leading to the development of flexible, cheaper, and more efficient thermoelectric generators.
ENERGY & ENVIRONMENTAL MATERIALS
(2023)
Article
Chemistry, Analytical
Kyuseok Lee, Gyeoungsun Song, Jungwook Kwon, Jihyeon Kim, Haesik Yang
Summary: In this study, a zwitterionic phenazine compound, mPPS, was used as an organic catalyst and electron mediator for the oxidation of NADH and the detection of glucose and LDH. mPPS exhibited high stability in phosphate buffered saline and its reduced form displayed good water solubility.
Article
Chemistry, Multidisciplinary
Paulo Henrique M. Buzzetti, Pierre-Yves Blanchard, Emerson Marcelo Girotto, Yuta Nishina, Serge Cosnier, Alan Le Goff, Michael Holzinger
Summary: A series of polycyclic aromatics were immobilized on carbon nanotube electrodes and electro-oxidized in aqueous solutions. The obtained quinones were characterized for mediated electron transfer in catalytic glucose oxidation with FAD dependent glucose dehydrogenase.
CHEMICAL COMMUNICATIONS
(2021)
Article
Materials Science, Multidisciplinary
Rizwan Khan, Keita Miyagawa, Alberto Bianco, Yuta Nishina
Summary: The covalent double functionalization of graphene oxide is an effective method to tune the properties of graphene-like materials, allowing the design of a multifunctional GO platform for applications in fuel cells as an electrolyte membrane and in supercapacitors as an electrode material.
APPLIED MATERIALS TODAY
(2021)
Article
Chemistry, Multidisciplinary
Bishal Boro, Mrinal K. Adak, Sohag Biswas, Chitra Sarkar, Yogendra Nailwal, Abhijit Shrotri, Biswarup Chakraborty, Bryan M. Wong, John Mondal
Summary: The rational synthesis of durable and efficient electrocatalysts for the oxygen evolution reaction (OER) is essential for renewable energy storage. This study developed a Ru@Bpy-POP catalyst that overcomes the drawbacks of commercial RuO2 catalysts, including low stability, catalyst deactivation, and high cost. The Ru@Bpy-POP catalyst exhibited promising OER activity with low overpotential and high current density, and the exceptional activity was attributed to the covalent integration of distinct Ru sites into the framework.
Article
Chemistry, Analytical
Ilaria Sorrentino, Marie Carriere, Helene Jamet, Ilaria Stanzione, Alessandra Piscitelli, Paola Giardina, Alan Le Goff
Summary: We investigated the use of POXA1b laccase from Pleurotus ostreatus for the oxidation of anthracene into anthraquinone. Different pathways can occur depending on the redox mediator combined to laccase, leading to different isomers. Carbon nanotubes were shown to act as redox mediators for anthracene oxidation into 9,10-anthraquinone, which was corroborated by theoretical calculations. The efficient adsorption of anthraquinones at CNT electrodes allows for low limit-of-detection of anthracene using laccase in solution, CNT-supported laccase, or laccase immobilized at magnetic beads.
Article
Chemistry, Analytical
Li-Ping Zong, Junji Li, Guofang Shu, Xinye Liu, Robert S. Marks, Xue-Ji Zhang, Serge Cosnier, Dan Shan
Summary: A novel ECL amplification strategy was established by designing Fe-N-C nanosheets with exceptional catalytic activity for O-2 activation. The material was successfully applied for antibiotic detection, indicating promising prospects for biological analysis and environmental monitoring.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Jeremy Schild, Bertrand Reuillard, Adina Morozan, Pascale Chenevier, Edmond Gravel, Eric Doris, Vincent Artero
Summary: Integration of a DuBois type Ni bioinspired molecular catalyst onto the surface of a carbon nanotube modified gas diffusion layer through noncovalent binding allowed for precise control over catalytic site loading. By adjusting the hydration level of the active layer, record current densities were achieved with the PGM-free anode at different temperatures. These results highlight the relevance of molecular approaches in developing electrocatalytic platforms for energy conversion.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Polymer Science
Monica Brachi, Paulo Henrique M. Buzzetti, Karine Gorgy, Dan Shan, Pierre Audebert, Alan le Goff, Hong Li, Redouane Borsali, Serge Cosnier
Summary: In this study, fluorescent organic 2,5,8-tris((adamantan-1-yl)-methoxy)-heptazine (HTZ-Ad) was solubilized in water by inclusion of adamantane groups into free beta-cyclodextrins or a cyclodextrin shell of glyconanoparticles. The modified nanoparticles showed fluorescence emission both in aqueous media and in their adsorbed state, providing the first spectroscopic characterization of a trialkoxyheptazine in aqueous medium. The binding of the glyconanoparticles to a surface was achieved through host-guest interactions with an electrochemically generated poly(pyrrole-adamantane) film, resulting in spatially controlled fluorescence.
Article
Chemistry, Multidisciplinary
Umberto Contaldo, Mathieu Curtil, Julien Perard, Christine Cavazza, Alan Le Goff
Summary: An AcPyTACN compound was synthesized for immobilizing recombinant CODH on carbon-nanotube electrodes, resulting in high current density and stability for electroenzymatic CO2 reduction.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Paulo Henrique M. Buzzetti, Anastasiia Berezovska, Yannig Nedellec, Serge Cosnier
Summary: This article describes a new concept of a hollow electrode based on the assembly of two buckypapers, which can contain a biocatalyst in a microcavity. The experimental results show that the hollow bioelectrodes have good storage stability and electroenzymatic activity in aqueous solution.
Article
Chemistry, Multidisciplinary
Afridi Zamader, Bertrand Reuillard, Jacques Pecaut, Laurent Billon, Antoine Bousquet, Gustav Berggren, Vincent Artero
Summary: Surface integration of molecular catalysts inspired by hydrogenase enzymes' active sites offers a promising approach for developing noble metal-free and sustainable H2 production technologies. In this study, a diironhexacarbonyl complex with two pyrene groups per catalytic unit was prepared and electrochemically characterized, allowing for its smooth integration onto multiwalled carbon nanotube-based electrodes through pi-interactions. The grafted catalyst achieved turnover numbers for H2 production of up to 4 +/- 2x10(3) within 20 hours of bulk electrolysis at neutral pH.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Chemistry, Inorganic & Nuclear
Alan Le Goff, Deborah Brazzolotto, Yannig Nedellec, Christian Philouze, Michael Holzinger, Fabrice Thomas
Summary: A new ligand and complex were designed and immobilized on multiwalled carbon nanotube electrodes. These complexes exhibit strong autoreduction tendencies and interact strongly with the sidewalls of the nanotubes. Additionally, they demonstrate good catalytic performance in oxygen reduction reactions.
INORGANIC CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Li-Ping Zong, Xiaozhong Chen, Dunru Zhu, Xi-Jie Li, Feng Li, Serge Cosnier, Xue-Ji Zhang, Robert S. Marks, Dan Shan
Summary: A novel electrochemiluminescence amplification strategy was developed using macrocyclic Schiff base Fe complexes, which showed remarkable redox electrocatalytic properties and enhanced ECL efficiency. The strategy was successfully applied in the detection of neomycin, highlighting its potential in biological analysis and environmental monitoring.
Article
Chemistry, Multidisciplinary
Michael G. Allan, Thomas Pichon, Jade A. McCune, Christine Cavazza, Alan Le Goff, Moritz F. Kuhnel
Summary: This work demonstrates the performance of [NiFeSe] hydrogenase from Desulfomicrobium baculatum for solar-driven hydrogen generation in a variety of organic-based deep eutectic solvents. It shows that the hydrogenase exhibits remarkable performance under aerobic conditions in these solvents when paired with a TiO2 photocatalyst. The tuning of water content further enhances hydrogen evolution activity and quantum yield. Contrary to common belief, these findings suggest that placing natural hydrogenases in non-natural environments can enhance their intrinsic activity, opening possibilities for full water splitting using hydrogenases.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Umberto Contaldo, David Roura Padrosa, Helene Jamet, Martin Albrecht, Francesca Paradisi, Alan Le Goff
Summary: The study demonstrates the immobilization of copper-containing nitrite reductase (NiR) from Alcaligenes faecalis on functionalised multi-walled carbon nanotube (MWCNT) electrodes. The immobilization is mainly driven by hydrophobic interactions, promoted by the modification of MWCNTs with adamantyl groups. The direct electrochemistry shows high bioelectrochemical reduction of nitrite at the redox potential of NiR with a high current density of 1.41 mA cm(-2). Furthermore, the immobilization-induced desymmetrization of the trimer causes an independent electrocatalytic behavior for each subunit, which is supported by an electron-tunneling distance dependence.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Inorganic & Nuclear
Jeireimy Domergue, Pawel Guinard, Magali Douillard, Jacques Pecaut, Sarah Hostachy, Olivier Proux, Colette Lebrun, Alan Le Goff, Pascale Maldivi, Carole Duboc, Pascale Delangle
Summary: To control the cellular level of reactive oxygen species (ROS) and prevent pathologies related to oxidative stress, designing antioxidants based on natural enzymes involved in ROS degradation is a promising strategy. In this study, Ni complexes with tripeptides derived from the ATCUN motif were synthesized to mimic the active site of nickel superoxide dismutase (NiSOD). Six different Ni(II) complexes with various coordination modes were characterized and found to exhibit SOD-like activity. The complexes with equilibrium between N-coordination and S-coordination showed the highest efficiency, indicating the beneficial effect of a nearby proton relay. This study also demonstrated that two bound thiolates and two amidates are not prerequisites for the catalytic activity of NiSOD mimics.
INORGANIC CHEMISTRY
(2023)
Article
Chemistry, Physical
Umberto Contaldo, Solene Gentil, Elise Courvoisier-Dezord, Pierre Rousselot-Pailley, Fabrice Thomas, Thierry Tron, Alan Le Goff
Summary: We report the unprecedented use of laccase to functionalize phenol-modified carbon nanotubes (CNTs). Enzymatically-generated metallopolyphenols or the laccase itself can be mildly and efficiently immobilized using the ability of laccase to generate phenoxyl radicals. Electrochemistry, XPS and EPR spectroscopy were used to assess the enzyme-catalyzed CNT functionalization. The efficient immobilization of laccase is confirmed by the high direct bioelectrocatalytic reduction of oxygen with a maximum current density of 1.95 mA cm(-2).
JOURNAL OF MATERIALS CHEMISTRY A
(2023)
Article
Chemistry, Physical
Monica Brachi, Fabien Giroud, Serge Cosnier, Alan Le Goff
Summary: In this study, we report a method to modify carbon nanotubes using alkyne-modified diazonium salts and alkyne-modified pyrene, followed by photo-irradiated thiol-yne click reaction. The results demonstrate that the photo-irradiation provides the highest reaction yields. The functionalized carbon nanotubes enable mediated bioelectrocatalytic glucose oxidation under low overpotential and high current density conditions.
JOURNAL OF MATERIALS CHEMISTRY A
(2023)
Article
Chemistry, Physical
Nathan Coutard, Bertrand Reuillard, Tran Ngoc Huan, Fabrice Valentino, Reuben T. Jane, Solene Gentil, Eugen S. Andreiadis, Alan Le Goff, Tristan Asset, Frederic Maillard, Bruno Jousselme, Adina Morozan, Sandrine Lyonnard, Vincent Artero, Pascale Chenevier
Summary: This study demonstrates the application of molecular-engineered bio-inspired catalysts in proton-exchange membrane fuel cells and the impact on performance, highlighting the need for redesigning the entire material system when implementing unconventional catalysts in catalytic layers. By optimizing the catalytic layer formulation, a significant enhancement in the performance of noble-metal-free PEMFCs was achieved.
Review
Chemistry, Multidisciplinary
Dan He, Yawen Li, Fuwen Zhao, Yuze Lin
Summary: High trap density in organic solar cells leads to localized charge carriers and reduced carrier lifetime, limiting device efficiency. This feature article summarizes the recent advances of trap suppression by material design and device engineering.
CHEMICAL COMMUNICATIONS
(2024)
Review
Chemistry, Multidisciplinary
Olivier Monfort, Arshitha Madhusudhan, Martin Motola
Summary: This article presents the advantages of coupling photocatalysis and Fenton-based processes for environmental remediation and discusses their synergy. It critically examines the simultaneous triggering of these two processes and provides insights into research approaches. The study aims to enhance understanding of this complex process and highlight the potential integration of new catalysts in current wastewater treatment technology.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Manideepa Dhar, Chittaranjan Mishra, Avijit Das, Uttam Manna
Summary: An approach of 'polymerization of monomers in its aggregated form' has been introduced to tailor the water wettability of fibrous and porous substrates, achieving hydrophobicity to superhydrophobicity transition, as well as patterned wettability. This facile chemical method provides a durable coating with adjustable and patterned wettability for various potential applications.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Saswat Ranjan Bhoi, Chhanda Debnath, Shikha Gandhi
Summary: In this article, a novel catalytic reaction using Pd(0) and Bronsted acid is reported for the synthesis of biologically important imidazolidinone-fused N-heterocycles through redox-neutral intramolecular N-allylation of indoles and pyrroles with alkynes. This atom-economical method is applicable to a wide range of substrates and eliminates the need for leaving groups or oxidizing agents commonly used in traditional allylation reactions.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Longkun Chen, Mingshuai Zhang, Meichen Liu, Zhuoyuan Liu, Yuetong Qiu, Zhilai Zhang, Fuchao Yu, Jiuzhong Huang
Summary: In this study, an efficient Rh(III)-catalyzed selective mono- and dual-C-H bond functionalization/cyclization with iodonium ylide as the sole coupling partner was demonstrated. Fused benzodiazepine skeletons were obtained in excellent yields, providing an improved approach to dual C-H unsymmetrical functionalization.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Alice Kennett, Sven Epple, Gabriella van der Valk, Irene Georgiou, Evelyne Gout, Romain R. Vives, Angela J. Russell
Summary: This study presents the design and synthesis of sulfated disaccharide inhibitors based on IdoA(2S)-GlcNS(6S), which showed potent inhibition of Sulf-2. The results suggest that IdoA(2S)-GlcNS(6S) is the shortest fragment size required for effective inhibition of Sulfs, and a trisulfated disaccharide is identified as the minimal fragment size of heparan sulfate for effective endosulfatase inhibition.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Mauro Garbini, Andrea Brunetti, Riccardo Pedrazzani, Magda Monari, Massimo Marcaccio, Giulio Bertuzzi, Marco Bandini
Summary: The self-adaptability of galvanostatic electrolysis was found to be helpful in a multistage chemo- and diastereoselective electrochemically promoted cyclodimerization of chalcones. Through a series of reductive events, densely functionalized cyclopentanes with five contiguous stereocenters were obtained (25 examples, yields up to 95%, dr values up to >20:1). Further experimental and electrochemical investigations indicated the crucial role of dynamic kinetic resolution of the aldol intermediate in the reaction mechanism.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Dhananjay S. Nipate, Neha Meena, Prakash N. Swami, Krishnan Rangan, Anil Kumar
Summary: In this study, the synthesis of functionalized benzo[a]phenazines and indazolo[2,3-a]quinolines was achieved through Rh(III)-catalyzed oxidative annulation of 2-arylquinoxalines and 2-aryl-2H-indazoles with allyl alcohols. The method showed a wide substrate scope, excellent functional group tolerance, good to high yields of annulated products, and the capability for scaled-up synthesis. A tentative mechanism of the annulation reaction was proposed based on a preliminary mechanistic investigation.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Ruonan Zhang, Abudukadi Tudi, Xia Yang, Xuping Wang, Zhihua Yang, Shujuan Han, Shilie Pan
Summary: A new lead tellurium borate material with a unique fundamental building block [B4O10] was synthesized and found to exhibit high birefringence. The structure-property relationship was discussed using first-principles calculations.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Amresh Kumar Singh, Baishali Thakurta, Anupam Giri, Monalisa Pal
Summary: This study introduces three key concepts for the quick synthesis of large-scale high-quality 2D materials films and their utilization in device fabrication.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Reina Hagiwara, Shun Nishimura, Kosuke Okeyoshi
Summary: A copolymer-conjugated nanocatalytic system has been developed to improve electron transfer and enhance photoinduced H2 generation.
CHEMICAL COMMUNICATIONS
(2024)
