Article
Biochemical Research Methods
Liuya Wei, Xianglong Kong, Mengran Wang, Yixin Zhang, Ruiyan Pan, Yuanzheng Cheng, Zhihua Lv, Jin Zhou, Jingjing Ming
Summary: The phosphorylation process of DNA by T4 polynucleotide kinase (T4 PNK) plays a crucial role in various DNA processes. This study presents a label-free T4 PNK fluorescence sensor based on split dimeric G4 and ligation-induced dimeric G4/thioflavin T (ThT) conformation. The sensor offers a sensitive detection range without interference from multiple cations.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Shujie Wu, Songen Wang, Zongbing Li, Chun Wu, Dik-Lung Ma, Xiangmin Miao
Summary: In this work, an iridium(III) complex was synthesized and its selective interaction with a specific G-quadruplex DNA sequence was studied. Results showed that the complex exhibits high selectivity and can be used as an efficient electrochemiluminescence probe for the detection of double-stranded DNA.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Jingyi Si, Wei Zhou, Ying Fang, Da Zhou, Yifan Gao, Qunyan Yao, Xizhong Shen, Changfeng Zhu
Summary: A simple and label-free method was developed to detect PNK activity and screen inhibitors, which can be used for predicting and improving tumor treatment sensitivity. This method successfully detected PNK activity in complex biological matrix and showed great potential in clinical practice.
Article
Chemistry, Analytical
Jinhua Yang, Guihua He, Wenying Wu, Wenfang Deng, Yueming Tan, Qingji Xie
Summary: In this study, we developed a photoelectrochemical (PEC) biosensor using Au nanoparticles (AuNPs)/SnS2/ZnIn2S4 as the active material, for sensitive detection of T4 polynucleotide kinase (T4 PNK) based on an enzymatic reaction induced DNA structure switch strategy. The PEC biosensor exhibited high performance with a wide detection range and low detection limit, and can also be used for screening enzyme inhibitors.
Article
Chemistry, Physical
Zhigang Liang, Xinyang Li, Chen Chen, Feifei Xu, Guanhong Xu, Fangdi Wei, Jing Yang, Qin Hu, Jianjun Zou, Yao Cen
Summary: We developed an efficient and label-free fluorescent biosensor for detecting T4 polynucleotide kinase (T4 PNK) using CRISPR/Cas12a and poly T-templated copper nanoparticles (CuNPs). The biosensor utilized single-stranded DNA (ssDNA) of poly T as a template for CuNPs synthesis. By combining Cas12a/crRNA with dsDNA, Cas12a was activated and prevented the synthesis of CuNPs. However, T4 PNK phosphorylated the 5' termini of dsDNA, degrading it and preventing the activation of Cas12a. This allowed the formation of CuNPs for the detection of T4 PNK in an isothermal and label-free manner. The sensing platform showed a low limit of detection and potential for broader applications.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Xi Zhou, Doudou Zhang, Ying Yan, Hailun He, Yukui Zhou, Changbei Ma
Summary: A label-free fluorescent method for detecting glutathione (GSH) based on a thioflavin T/G-quadruplex conformational switch is developed, showing high sensitivity and selectivity. The method can also be applied to detect other amino acids with satisfactory efficacy.
Article
Spectroscopy
Wanling Cui, Xiaoyang Fan, Wenqi Zhao, Jinrong Liu, Liangjie Zheng, Libing Zhou, Junye Zhang, Xiumei Zhang, Xiaoxin Wang
Summary: This paper presents a label-free fluorescent biosensor for amplified detection of T4 polynucleotide kinase (PNK) activity. The biosensor combines rolling circle amplification (RCA) and catalytic hairpin assembly (CHA) to achieve high amplification efficiency and can detect T4 PNK as low as 6.63 x 10-4 U mL-1. The biosensor has been successfully applied to detect T4 PNK activity in cell lysates and shows great potential in drug screening and disease treatment.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Applied
Li-Xia Wang, Jing-Tao Zhang, Xin Sun, Da-Wei Yang, Ya-Lin Tang
Summary: Chiral supramolecules exhibit superior recognition and catalytic abilities due to their chiral and self-assembly features. The constructed chiral H-aggregates in this study can specifically recognize DNA G-quadruplexes and enhance fluorescence intensity through binding of MeCy monomer.
Article
Chemistry, Analytical
Yuqi Zhang, Qianqian Cai, Xiaoshi Yan, Guifen Jie
Summary: The development of DNA nanomachines offers a new approach for tumor marker detection. In this study, an intelligent three-dimensional DNA walking machine coupled with a unique nanomachine was designed for versatile fluorescence detection of T4 PNK activity and mRNA. The DNA nanomachine consisted of a free DNA walker and DNA nanowire cascade amplification reaction, enabling sensitive and specific detection of PNK and mRNA. This novel fluorescence nanomachine holds promise for versatile target detection and potential applications in molecular-targeted therapies and clinical diagnostics.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Organic
Yu Wang, Chunjie Li, Xueyu Hao, Liangpeng Wang, Xiaoye Ma, Rizhe Jin, Chuanqing Kang, Lianxun Gao
Summary: This study presented a non-covalent bond approach to construct G-quadruplex (GQ) ligands with an extended planar structure, stabilizing GQs and inducing conformational conversion of hybrid GQs through quadruplex intermolecular hydrogen bonds. The ligands exhibited moderate cytotoxicity against HeLa cells and effectively induced growth inhibition and apoptosis. These results demonstrate the feasibility of quickly building GQ ligands through intermolecular interactions, aiding in ligand design and establishing a ligand library efficiently.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)
Article
Spectroscopy
Yongqiang Kang, Chunying Wei
Summary: Recent studies have shown significant interest in small molecule fluorescent probes for detecting G-quadruplexes DNA. In this paper, three benzothiazole derivatives named 2a-2c were synthesized and studied for their interactions with various forms of DNA and distribution in living cells. These compounds exhibited selective and sensitive turn-on fluorescence responses for G-quadruplex DNA, particularly in the mitochondria, with low cytotoxicity. The compounds showed affinity towards G-quadruplex DNA mainly through end-stacking mode, inducing conformational changes and folding of the DNA structures.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Spectroscopy
Tuo Zhang, Yanmei Shen, Jia Ge, Weixia Wang, Lingbo Qu, Zhaohui Li
Summary: This study presents a novel biosensor based on PDANTs-mediated FRET for detecting the activity of T4 PNKP. The fluorescence quenching and retention are utilized to measure the dephosphorylation activity of T4 PNKP, showing high sensitivity for practical detection in cell lysates.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
Jie Sun, Aohuan Guo, Menghua Yan, Xiuming Wu, Guang-Li Wang
Summary: In this study, a novel nanozyme was successfully constructed to detect the activity of T4 polynucleotide kinase (T4 PNK) using Kanamycin (KANA) as a trigger and an amplified colorimetric method. The assay showed a detection range of 1.0 x 10-3 to 10.0 U/mL and a limit of detection (LOD) of 1.42 x 10-4 U/mL, with satisfactory performance in serum samples. The method also demonstrated the advantages of operational convenience, no labeling or immobilization process, and no costly instrument requirement.
Article
Chemistry, Analytical
Ran Luo, Guowei Lian, Hengxuan Li, Houyu Han, Dianming Zhou, Xiaoqun Gong
Summary: In this study, a highly sensitive and selective transcription-based signal amplification method was developed for trace nucleic acid detection. The method utilizes the advantages of long template synthesis and self-amplification, and can be further modified for the detection of other analytical targets.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Wanling Cui, Xiaoyang Fan, Congcong Shen, Qingshuai Chen, Xiumei Zhang, Enguang Lv, Chonghui Li, Qiang Li, Rui Wang, Shicai Xu, Jihua Wang
Summary: In this study, a multiple primers-mediated exponential rolling circle amplification strategy was proposed for label-free and highly sensitive fluorescent analysis of T4 PNK and T4 DNA ligase activity. The strategy showed extremely high detection sensitivity and exhibited excellent inhibitor evaluation effects. Moreover, it could detect T4 PNK in HeLa cell lysate, indicating its potential application in disease diagnosis and treatment evaluation.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Kasipandi Vellaisamy, Guodong Li, Chung-Nga Ko, Hai-Jing Zhong, Sarwat Fatima, Hiu-Yee Kwan, Chun-Yuen Wong, Wai-Jing Kwong, Weihong Tan, Chung-Hang Leung, Dik-Lung Ma
Article
Materials Science, Biomaterials
Jin-Biao Liu, Kasipandi Vellaisamy, Guodong Li, Chao Yang, Suk-Yu Wong, Chung-Hang Leung, Shou-Zhi Pu, Dik-Lung Ma
JOURNAL OF MATERIALS CHEMISTRY B
(2018)
Review
Chemistry, Inorganic & Nuclear
Tian-Shu Kang, Jia-Tong Zhang, Kasipandi Vellaisamy, Dik-Lung Ma, Chung-Hang Leung
DALTON TRANSACTIONS
(2018)
Correction
Biochemical Research Methods
Nagarjun Narayanaswamy, Kasturi Chakraborty, Anand Saminathan, Elizabeth Zeichner, KaHo Leung, John Devany, Yamuna Krishnan
Article
Biochemical Research Methods
Nagarjun Narayanaswamy, Kasturi Chakraborty, Anand Saminathan, Elizabeth Zeichner, KaHo Leung, John Devany, Yamuna Krishnan
Article
Nanoscience & Nanotechnology
KaHo Leung, Kasturi Chakraborty, Anand Saminathan, Yamuna Krishnan
NATURE NANOTECHNOLOGY
(2019)
Editorial Material
Chemistry, Multidisciplinary
KaHo Leung, Yamuna Krishnan
ACS CENTRAL SCIENCE
(2019)
Review
Biochemistry & Molecular Biology
Chung-Hang Leung, Jia-Tong Zhang, Guan-Jun Yang, Hao Liu, Quan-Bin Han, Dik-Lung Ma
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Review
Biochemistry & Molecular Biology
Ke-Jia Wu, Pui-Man Lei, Hao Liu, Chun Wu, Chung-Hang Leung, Dik-Lung Ma
Article
Cell Biology
James Osei-Owusu, Junhua Yang, Ka Ho Leung, Zheng Ruan, Wei Lu, Yamuna Krishnan, Zhaozhu Qiu
Summary: The proton-activated Cl- (PAC) channel has been identified as a low pH sensor in endosomes, preventing hyper-acidification by releasing Cl- from the lumen.
Article
Chemistry, Medicinal
Shasha Cheng, Yi Kuang, Guodong Li, Jia Wu, Chung-Nga Ko, Wanhe Wang, Dik-Lung Ma, Min Ye, Chung-Hang Leung
Summary: This study reports the use of dehydroeburicoic acid (DEA) as a dual inhibitor of the Keap1-Nrf2 protein-protein interaction (PPI) and GSK3 beta, providing potential therapeutic strategies for alcoholic liver disease (ALD). DEA disrupts the Keap1-Nrf2 PPI and restores Nrf2 activity, leading to the activation of antioxidant genes. It also alleviates ethanol-induced mitochondrial dysfunction and exhibits minimal toxicity in the ALD cell model.
Review
Chemistry, Inorganic & Nuclear
Wanhe Wang, Jianhua Liu, Sang-Cuo Nao, Dik-Lung Ma, Jing Wang, Chung-Hang Leung
Summary: Changes in protein levels and types can indicate the occurrence of diseases, making differentially expressed proteins useful biomarkers for diagnosis and treatment. Luminescent iridium(III) complexes, especially affinity-based ones, with excellent optical properties, have gained attention in protein-based disease diagnostics. This review summarizes the current advances and future perspectives of affinity-based luminescent iridium(III) complexes for detecting disease-related proteins.
Meeting Abstract
Oncology
Priyanka Gupta, Kiranj Chaudagar, Sweta Sharma-Saha, Kaela Bynoe, Lea Maillat, Brian Heiss, KaHo Leung, Yamuna Krishnan, Walter Stadler, Akash Patnaik
Review
Chemistry, Analytical
Dik-Lung Ma, Chun Wu, Guodong Li, Chung-Hang Leung
JOURNAL OF ANALYSIS AND TESTING
(2018)
Article
Chemistry, Multidisciplinary
Sze-Wing Ng, Lai-Hon Chung, Chi-Fung Yeung, Hoi-Shing Lo, Hau-Lam Shek, Tian-Shu Kang, Chung-Hang Leung, Dik-Lung Ma, Chun-Yuen Wong
CHEMISTRY-A EUROPEAN JOURNAL
(2018)
Review
Chemistry, Multidisciplinary
Dan He, Yawen Li, Fuwen Zhao, Yuze Lin
Summary: High trap density in organic solar cells leads to localized charge carriers and reduced carrier lifetime, limiting device efficiency. This feature article summarizes the recent advances of trap suppression by material design and device engineering.
CHEMICAL COMMUNICATIONS
(2024)
Review
Chemistry, Multidisciplinary
Olivier Monfort, Arshitha Madhusudhan, Martin Motola
Summary: This article presents the advantages of coupling photocatalysis and Fenton-based processes for environmental remediation and discusses their synergy. It critically examines the simultaneous triggering of these two processes and provides insights into research approaches. The study aims to enhance understanding of this complex process and highlight the potential integration of new catalysts in current wastewater treatment technology.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Manideepa Dhar, Chittaranjan Mishra, Avijit Das, Uttam Manna
Summary: An approach of 'polymerization of monomers in its aggregated form' has been introduced to tailor the water wettability of fibrous and porous substrates, achieving hydrophobicity to superhydrophobicity transition, as well as patterned wettability. This facile chemical method provides a durable coating with adjustable and patterned wettability for various potential applications.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Saswat Ranjan Bhoi, Chhanda Debnath, Shikha Gandhi
Summary: In this article, a novel catalytic reaction using Pd(0) and Bronsted acid is reported for the synthesis of biologically important imidazolidinone-fused N-heterocycles through redox-neutral intramolecular N-allylation of indoles and pyrroles with alkynes. This atom-economical method is applicable to a wide range of substrates and eliminates the need for leaving groups or oxidizing agents commonly used in traditional allylation reactions.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Longkun Chen, Mingshuai Zhang, Meichen Liu, Zhuoyuan Liu, Yuetong Qiu, Zhilai Zhang, Fuchao Yu, Jiuzhong Huang
Summary: In this study, an efficient Rh(III)-catalyzed selective mono- and dual-C-H bond functionalization/cyclization with iodonium ylide as the sole coupling partner was demonstrated. Fused benzodiazepine skeletons were obtained in excellent yields, providing an improved approach to dual C-H unsymmetrical functionalization.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Alice Kennett, Sven Epple, Gabriella van der Valk, Irene Georgiou, Evelyne Gout, Romain R. Vives, Angela J. Russell
Summary: This study presents the design and synthesis of sulfated disaccharide inhibitors based on IdoA(2S)-GlcNS(6S), which showed potent inhibition of Sulf-2. The results suggest that IdoA(2S)-GlcNS(6S) is the shortest fragment size required for effective inhibition of Sulfs, and a trisulfated disaccharide is identified as the minimal fragment size of heparan sulfate for effective endosulfatase inhibition.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Mauro Garbini, Andrea Brunetti, Riccardo Pedrazzani, Magda Monari, Massimo Marcaccio, Giulio Bertuzzi, Marco Bandini
Summary: The self-adaptability of galvanostatic electrolysis was found to be helpful in a multistage chemo- and diastereoselective electrochemically promoted cyclodimerization of chalcones. Through a series of reductive events, densely functionalized cyclopentanes with five contiguous stereocenters were obtained (25 examples, yields up to 95%, dr values up to >20:1). Further experimental and electrochemical investigations indicated the crucial role of dynamic kinetic resolution of the aldol intermediate in the reaction mechanism.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Dhananjay S. Nipate, Neha Meena, Prakash N. Swami, Krishnan Rangan, Anil Kumar
Summary: In this study, the synthesis of functionalized benzo[a]phenazines and indazolo[2,3-a]quinolines was achieved through Rh(III)-catalyzed oxidative annulation of 2-arylquinoxalines and 2-aryl-2H-indazoles with allyl alcohols. The method showed a wide substrate scope, excellent functional group tolerance, good to high yields of annulated products, and the capability for scaled-up synthesis. A tentative mechanism of the annulation reaction was proposed based on a preliminary mechanistic investigation.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Ruonan Zhang, Abudukadi Tudi, Xia Yang, Xuping Wang, Zhihua Yang, Shujuan Han, Shilie Pan
Summary: A new lead tellurium borate material with a unique fundamental building block [B4O10] was synthesized and found to exhibit high birefringence. The structure-property relationship was discussed using first-principles calculations.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Amresh Kumar Singh, Baishali Thakurta, Anupam Giri, Monalisa Pal
Summary: This study introduces three key concepts for the quick synthesis of large-scale high-quality 2D materials films and their utilization in device fabrication.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Reina Hagiwara, Shun Nishimura, Kosuke Okeyoshi
Summary: A copolymer-conjugated nanocatalytic system has been developed to improve electron transfer and enhance photoinduced H2 generation.
CHEMICAL COMMUNICATIONS
(2024)
