Article
Chemistry, Analytical
Yanan Zhou, Yan Zeng, Dun Zhang, Peng Qi, Yingwen Wang, Yue Xin, Yan Sun
Summary: We have developed a simple and highly sensitive method for detecting bacterial DNA. By utilizing target-aided self-assembly cycle amplification coupled with DNA-AgNCs/three-way DNA junction, we successfully detected target DNA at concentrations as low as 19.95 fM, with good selectivity and recovery rate.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Hua Chai, Peng Miao
Summary: A novel electrochemical strategy combining strand displacement amplification and DNA nanostructures was developed for ultrasensitive detection of ctDNA, achieving a limit of detection as low as 48 amol/L. This proposed electrochemical biosensor shows great potential for clinical applications.
CHINESE CHEMICAL LETTERS
(2021)
Review
Chemistry, Analytical
Hamed Zahraee, Zahra Khoshbin, Fatemeh Mohammadi, Mansour Mashreghi, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: Nucleic acids can form highly regular and complex structures at the nanoscale through self-assembly and programmability. The smallest type of junction, known as three-way junction (TWJ), can be formed by hybridizing three DNA or RNA strands. TWJ nanostructures provide multifunctional sites for biomolecular nanoarchitectures. This study discusses the achievements and future potential of TWJ-based biosensors, specifically aptasensors, for monitoring different targets.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Zhiqiang Sun, Tommy Stormberg, Shaun Filliaux, Yuri L. Lyubchenko
Summary: The study utilized three-way DNA junctions as DNA labels for determining the protein's location on DNA substrates in AFM images. High-speed AFM experiments revealed a high dynamic of nucleosomes which could spontaneously unravel and disassemble.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Zishan Feng, Wei Zhang, Longjie Li, Bocheng Tu, Weicong Ye, Xiaofeng Tang, Hongbo Wang, Xianjin Xiao, Tongbo Wu
Summary: A new method for detecting low-abundance mutations was developed using a DNA three-way junction structure and optimization of an assistant strand. The method showed improved selectivity for mutant-type DNA and cost savings. The sensitivity and accuracy of the method were demonstrated in the detection of a human ovarian cancer-related gene mutation.
CHINESE CHEMICAL LETTERS
(2021)
Article
Chemistry, Multidisciplinary
Anders Marcher, Vipin Kumar, Veronica L. Andersen, Kassem El-Chami, Thuy J. D. Nguyen, Mads K. Skaanning, Imke Rudnik-Jansen, Jesper S. Nielsen, Kenneth A. Howard, Jorgen Kjems, Kurt V. Gothelf
Summary: Oligonucleotides are increasingly used as programmable connection materials for assembling molecules and proteins in well-defined structures. This study reports the application of aTNA, a type of oligonucleotide, in forming a highly stable four-way junction (4WJ) that allows easy assembly of components for in vivo treatment and imaging. The aTNA 4WJ is stable in serum, non-toxic, and does not induce an immune response.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Physical
Jie Shen, Wei Sun, Di Liu, Thomas Schaus, Peng Yin
Summary: Utilizing 3D DNA nanostructures, the line pitch has been scaled down to 16.2 nm with a critical dimension size of 7.2 nm, demonstrating an improvement in nanolithography resolution achieved through DNA modular epitaxy.
Article
Chemistry, Multidisciplinary
Antonio Garcia-Guerra, Ruth Ellerington, Jens Gaitzsch, Jonathan Bath, Mahnseok Kye, Miguel A. Varela, Giuseppe Battaglia, Matthew J. A. Wood, Raquel Manzano, Carlo Rinaldi, Andrew J. Turberfield
Summary: Nucleic acid therapeutics require effective delivery systems to overcome challenges such as accumulation in cells and escape from the endosomal pathway. Spherical nucleic acids (SNAs), which consist of a gold nanoparticle supported by a corona of oligonucleotides, have promising properties for nucleic acid delivery. However, they face vulnerability to lysosomal degradation or recycling exocytosis. In this study, an alternative SNA core based on a pH-sensitive polymer is investigated. These polymeric SNAs are used to deliver siRNA duplexes against a genetic target called C9orf72, achieving effective knock-down in motor neuron-like cells.
NANOSCALE ADVANCES
(2023)
Article
Chemistry, Analytical
Jingjing Ye, Jiaxi Gao, Weixiang Huang, Ruo Yuan, Wenju Xu
Summary: It is interesting to use the branched arms of three-way DNA junction (3WDJ) for modifying specific recognizing and/or sensing elements of multivariate analytes. In this study, a bivariate electrochemical biosensor was developed based on a functional 3WDJ, with two targeting DNA segments specific to SARS-CoV-2 as analyte models. The biosensor showed simplified detection, high sensitivity, and potentially more accurate electrochemical assay of multivariate targets.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemical Research Methods
Brionna McGorman, Simon Poole, Miguel Vazquez Lopez, Andrew Kellett
Summary: The development of compounds that can selectively bind with non-canonical DNA structures has expanded in recent years. In this study, a multiplex fluorescent polyacrylamide gel electrophoresis (PAGE) and microscale thermophoresis (MST) assays were used to evaluate junction-binding compounds. The combination of PAGE and MST provided complementary results for the visualization of junction formation and the determination of binding affinity, and can aid in the discovery and design of new therapeutics.
Article
Chemistry, Analytical
Ziheng Hu, Biying Zhao, Ping Miao, Xialing Hou, Feifei Xing, Yingying Chen, Lingyan Feng
Summary: An electrochemical biosensor based on TWJ DNA with LNA as capture probe and enzyme-free signal amplification strategy was developed for sensitive detection of miRNA-21. The sensor showed a linear range from 100 aM to 100 pM and a low detection limit of 77 aM for miRNA-21 detection, demonstrating promising application in direct determination of target miRNA-21 in biological fluids.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Chun-yan Qu, Li-sha Zhou, Li-hong Shu, Qian Huang
Summary: A stable tetrahedron-assisted probe was developed for the detection of multiple miRNAs, allowing simultaneous detection of miRNA-21 and miRNA-144. The method showed good agreement with qRT-PCR in clinical samples, suggesting significant potentials in early cancer diagnosis and treatment.
Article
Computer Science, Information Systems
Chengyong Jin, Bao Qing Hu
Summary: This paper introduces the concept of hesitant sets to unify different types of fuzzy sets. It discusses the construction of decision evaluation functions and three-way decisions based on hesitant sets in three-way decision spaces. The paper presents methods for constructing decision evaluation functions in semi-three-way and quasi-three-way decision spaces, as well as transformation methods to three-way decision spaces. The importance of these methods is supported by numerous examples.
INFORMATION SCIENCES
(2023)
Article
Biochemical Research Methods
Chunxu Yu, Siyan Zhou, Xin Zhao, Yidan Tang, Lina Wang, Baiyang Lu, Fanzheng Meng, Bingling Li
Summary: The nucleic acid testing technology has advanced in recent years, but there are still challenges in the clinical application of multiple nucleic acid assays, such as accuracy, speed, and cost. This paper introduces a three-way junction structure that can translate analytes of loop-mediated isothermal amplification to a catalytic hairpin assembly. With a well-optimized nucleic acid circuit, multiple and logical analysis can be easily achieved in a single reaction with high sensitivity and selectivity. The experimental results of clinical samples support the potential clinical application of this methodology.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jinmyeong Kim, Seung Woo Noh, Chulhwan Park, Jin-Ho Lee, Hyeon-Yeol Cho, Junhong Min, Taek Lee
Summary: This study presents a label-free electrochemical biosensor for the effective detection of TNF-alpha in human serum, offering a good dynamic range and sensitivity.
BIOELECTROCHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Remy Pawlak, J. G. Vilhena, Philipp D'Astolfo, Xunshan Liu, Giacomo Prampolini, Tobias Meier, Thilo Glatzel, Justin A. Lemkul, Robert Haner, Silvio Decurtins, Alexis Baratoff, Ruben Perez, Shi-Xia Liu, Ernst Meyer
Article
Chemistry, Organic
Jovana Jevric, Simon M. Langenegger, Robert Haner
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2020)
Article
Chemistry, Organic
Giovanni Picca, Markus Probst, Simon M. Langenegger, Oleg Khorev, Peter Butikofer, Anant K. Menon, Robert Haner
BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Remy Pawlak, Xunshan Liu, Silviya Ninova, Philipp D'Astolfo, Carl Drechsel, Sara Sangtarash, Robert Haner, Silvio Decurtins, Hatef Sadeghi, Colin J. Lambert, Ulrich Aschauer, Shi-Xia Liu, Ernst Meyer
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Biochemical Research Methods
Simon Rothenbuehler, Ioan Iacovache, Simon M. Langenegger, Benoit Zuber, Robert Haener
Summary: The precise arrangement of structural subunits is crucial for the proper shape and function of supramolecular assemblies. In this study, chemically modified DNA hybrids were assembled into various architectures using an amphiphilic DNA duplex as the building block. The morphology of the assemblies was determined by the chemical nature of the subunit, such as the length of polyethylene glycols or the attachment of a carbohydrate moiety. Cryo-electron microscopy revealed the arrangement of the DNA duplexes within the different constructs, showing a change in morphology from vesicles to ribbons with increasing chain length of polyethylene glycol. Interestingly, the attachment of a N-acetylgalactosamine carbohydrate resulted in a novel star-shaped architecture. These findings provide new insights into the field of DNA nanotechnology.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Chemistry, Organic
Jan Thiede, Simon Rothenbuhler, Ioan Iacovache, Simon M. Langenegger, Benoit Zuber, Robert Haner
Summary: This article presents the supramolecular self-assembly of pyrene-DNA conjugates into nanostructures. DNA functionalized with different types of pyrene isomers can self-assemble into nano-objects with various shapes. The research also constructs an artificial light-harvesting complex, which has implications for the design of photosynthetic devices.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Article
Chemistry, Organic
Simon Rothenbuehler, Adrian Gonzalez, Ioan Iacovache, Simon M. Langenegger, Benoit Zuber, Robert Haener
Summary: The supramolecular assembly of DNA conjugates with tetraphenylethylene (TPE) sticky ends into vesicular structures is studied in this research. The aggregation-induced emission (AIE) active TPE units are used to monitor the assembly process using fluorescence spectroscopy. The number of TPE modifications on the conjugates' overhangs influences the supramolecular assembly behavior, with a minimum of two TPE residues required for a well-defined assembly process. The designed DNA-based nanostructures offer customized functionalization for applications in DNA nanotechnology.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Jovana Jevric, Simon M. Langenegger, Robert Haener
Summary: This study describes the chemical synthesis and supramolecular assembly of an aromatic oligoamine, which leads to the formation of vesicular objects in aqueous solution. The assembly process is monitored by absorption and fluorescence spectroscopy, and the vesicles are characterized by atomic force and transmission electron microscopy. The electrostatic complementarity of anionic supramolecular polymer sheets and cationic vesicles is utilized for a layered assembly process.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Larysa Markova, Markus Probst, Robert Haener
Article
Chemistry, Multidisciplinary
Ping Zhou, Ulrich Aschauer, Silvio Decurtins, Thomas Feurer, Robert Haner, Shi-Xia Liu
CHEMICAL COMMUNICATIONS
(2020)
Article
Chemistry, Multidisciplinary
Simon Rothenbuehler, Ioan Iacovache, Simon M. Langenegger, Benoit Zuber, Robert Haener
Article
Chemistry, Organic
Nutcha Burki, Elena Grossenbacher, Andrea Cannizzo, Thomas Feurer, Simon M. Langenegger, Robert Haner
ORGANIC & BIOMOLECULAR CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Peter W. Muenich, Matthias Pfaeffli, Michel Volland, Shi-Xia Liu, Robert Haener, Dirk M. Guldi
Review
Chemistry, Multidisciplinary
Dan He, Yawen Li, Fuwen Zhao, Yuze Lin
Summary: High trap density in organic solar cells leads to localized charge carriers and reduced carrier lifetime, limiting device efficiency. This feature article summarizes the recent advances of trap suppression by material design and device engineering.
CHEMICAL COMMUNICATIONS
(2024)
Review
Chemistry, Multidisciplinary
Olivier Monfort, Arshitha Madhusudhan, Martin Motola
Summary: This article presents the advantages of coupling photocatalysis and Fenton-based processes for environmental remediation and discusses their synergy. It critically examines the simultaneous triggering of these two processes and provides insights into research approaches. The study aims to enhance understanding of this complex process and highlight the potential integration of new catalysts in current wastewater treatment technology.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Manideepa Dhar, Chittaranjan Mishra, Avijit Das, Uttam Manna
Summary: An approach of 'polymerization of monomers in its aggregated form' has been introduced to tailor the water wettability of fibrous and porous substrates, achieving hydrophobicity to superhydrophobicity transition, as well as patterned wettability. This facile chemical method provides a durable coating with adjustable and patterned wettability for various potential applications.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Saswat Ranjan Bhoi, Chhanda Debnath, Shikha Gandhi
Summary: In this article, a novel catalytic reaction using Pd(0) and Bronsted acid is reported for the synthesis of biologically important imidazolidinone-fused N-heterocycles through redox-neutral intramolecular N-allylation of indoles and pyrroles with alkynes. This atom-economical method is applicable to a wide range of substrates and eliminates the need for leaving groups or oxidizing agents commonly used in traditional allylation reactions.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Longkun Chen, Mingshuai Zhang, Meichen Liu, Zhuoyuan Liu, Yuetong Qiu, Zhilai Zhang, Fuchao Yu, Jiuzhong Huang
Summary: In this study, an efficient Rh(III)-catalyzed selective mono- and dual-C-H bond functionalization/cyclization with iodonium ylide as the sole coupling partner was demonstrated. Fused benzodiazepine skeletons were obtained in excellent yields, providing an improved approach to dual C-H unsymmetrical functionalization.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Alice Kennett, Sven Epple, Gabriella van der Valk, Irene Georgiou, Evelyne Gout, Romain R. Vives, Angela J. Russell
Summary: This study presents the design and synthesis of sulfated disaccharide inhibitors based on IdoA(2S)-GlcNS(6S), which showed potent inhibition of Sulf-2. The results suggest that IdoA(2S)-GlcNS(6S) is the shortest fragment size required for effective inhibition of Sulfs, and a trisulfated disaccharide is identified as the minimal fragment size of heparan sulfate for effective endosulfatase inhibition.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Mauro Garbini, Andrea Brunetti, Riccardo Pedrazzani, Magda Monari, Massimo Marcaccio, Giulio Bertuzzi, Marco Bandini
Summary: The self-adaptability of galvanostatic electrolysis was found to be helpful in a multistage chemo- and diastereoselective electrochemically promoted cyclodimerization of chalcones. Through a series of reductive events, densely functionalized cyclopentanes with five contiguous stereocenters were obtained (25 examples, yields up to 95%, dr values up to >20:1). Further experimental and electrochemical investigations indicated the crucial role of dynamic kinetic resolution of the aldol intermediate in the reaction mechanism.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Dhananjay S. Nipate, Neha Meena, Prakash N. Swami, Krishnan Rangan, Anil Kumar
Summary: In this study, the synthesis of functionalized benzo[a]phenazines and indazolo[2,3-a]quinolines was achieved through Rh(III)-catalyzed oxidative annulation of 2-arylquinoxalines and 2-aryl-2H-indazoles with allyl alcohols. The method showed a wide substrate scope, excellent functional group tolerance, good to high yields of annulated products, and the capability for scaled-up synthesis. A tentative mechanism of the annulation reaction was proposed based on a preliminary mechanistic investigation.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Ruonan Zhang, Abudukadi Tudi, Xia Yang, Xuping Wang, Zhihua Yang, Shujuan Han, Shilie Pan
Summary: A new lead tellurium borate material with a unique fundamental building block [B4O10] was synthesized and found to exhibit high birefringence. The structure-property relationship was discussed using first-principles calculations.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Amresh Kumar Singh, Baishali Thakurta, Anupam Giri, Monalisa Pal
Summary: This study introduces three key concepts for the quick synthesis of large-scale high-quality 2D materials films and their utilization in device fabrication.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Reina Hagiwara, Shun Nishimura, Kosuke Okeyoshi
Summary: A copolymer-conjugated nanocatalytic system has been developed to improve electron transfer and enhance photoinduced H2 generation.
CHEMICAL COMMUNICATIONS
(2024)
