Article
Engineering, Environmental
Ying Li, Kai Liu, Boxu Wang, Zheng Liu, Chuanyu Yang, Junyang Wang, Xinyue Ma, Hongxia Li, Chunyan Sun
Summary: Based on the high recognition ability and flexible programmability of GR5 DNAzyme, two fluorescent biosensors were developed for amplified detection of Pb2+ using Ti3C2TX MXenes and 2-aminopurine (2-AP). The sensors demonstrated simple procedures, high sensitivity, and good specificity, making them suitable for on-site monitoring of lead pollution in water samples.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Chemistry, Analytical
Yuan Zhang, Tianxiang Xue, Liangfen Cheng, Jiayi Wang, Ruitao Shen, Juan Zhang
Summary: A simple and low-cost biosensor has been developed for the detection of Cr3+ ion using the image processing function of a smartphone. The biosensor shows simplicity, affordability, high specificity, and anti-interference ability.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Yanju Chen, Hui Wu, Siwenjie Qian, Xiaoping Yu, Huan Chen, Jian Wu
Summary: A highly sensitive lead detection method combining DNAzyme and CRISPR system is proposed, which can detect low concentrations of lead ions and visualize the results with a portable 3D printing device, suitable for on-site detection.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemistry & Molecular Biology
Mingwei Liu, Jie Dong, Zhiguang Suo, Qixuan Wang, Min Wei, Baoshan He, Huali Jin
Summary: The presence of heavy metals in the ecological environment presents a serious threat to human health. Therefore, it is crucial to develop a simple and sensitive method for heavy metal detection. In this study, a Pb2+-DNAzyme immobilized on magnetic beads was successfully utilized to construct a fluorescent/electrochemical dual-mode biosensor. The dual-mode biosensor exhibited a good linear relationship between signal response and Pb2+ concentration, achieving accurate and reliable detection of Pb2+ and introducing a new approach for biosensing strategies. Notably, the sensor demonstrated high sensitivity and accuracy in the detection of Pb2+ in real sample analysis.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Xiaoyan Sun, Shilong Dong, Wenying Zhao
Summary: An ultrasensitive electrochemical biosensor based on catalytic hairpin assembly and DNAzyme signal amplification strategy is proposed for the detection of lead ions. The biosensor utilizes polyethyleneimine-reduced graphene oxide combined with gold@silver nanosheets as electrode substrate modification materials. The biosensor demonstrates a good linear relationship and low detection limit, enabling trace detection of lead ions in tap water samples and providing a platform for the detection of other targets.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biophysics
Junping Ma, Wushuang Bai, Jianbin Zheng
Summary: A self-cleaning electrochemical biosensor based on Cu-TCPP metal-organic framework and DNA nanomotors was developed for cyclic detection of Pb2+ ions. The sensor exhibits a wide linear range and low detection limit, and can selectively detect Pb2+ in the presence of other metal ions.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemical Research Methods
Dawei Li, Shen Ling, Xinru Cheng, Zhaoqi Yang, Bei Lv
Summary: A colorimetric biosensor assay has been developed for the detection of Cd2+ and Hg2+ based on Cd2+-dependent DNAzyme cleavage and Hg2+-binding-induced conformational switching of the G-quadruplex fragment. The assay exhibits excellent selectivity and high sensitivity, making it suitable for environmental water sample testing and further environmental monitoring.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Huaqin Guan, Shulin Yang, Cheng Zheng, Lingye Zhu, Shujuan Sun, Mengmeng Guo, Xuemei Hu, Xiaoying Huang, Liangxing Wang, Zhifa Shen
Summary: The study developed a tetrahedron-based Pb2+-sensitive DNAzyme sensor (TPS) for in vivo detection of Pb2+, which can quantitatively detect Pb2+ down to 1 nM without interference from other metal ions. The results showed that the sensor could preserve 85% of the fluorescence signal in different complex biological milieu.
Article
Chemistry, Analytical
Qi Luo, Lingfeng Qin, Peng Zhang, Bo Feng, Xiaosheng Ye, Taiping Qing, Zhihe Qing
Summary: Heavy metal ions are a serious source of water pollution, and their accurate detection is crucial for pollution control and ecological protection. This study presents a persistent luminescent nanobeacon that can detect metal ions in real samples by avoiding background interference.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Huali Jin, Ruike Liu, Tian Bai, Min Wei, Baoshan He, Zhiguang Suo
Summary: A low-noise ratiometric fluorescence biosensor based on Pb2+-dependent DNAzyme and exonuclease III (Exo III)-assisted cascade signal amplification was developed for the detection of lead ions. The biosensor exhibited a lower limit of detection and a wider detection range compared to other methods, and showed satisfactory results in the detection of lead ions in tap water, tea, and rice flour samples. This ratiometric fluorescence biosensor has great potential in various target monitoring.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yinyin Liu, Ruhan Qiu, Zhicheng Zhang, Dawei Chen, Yushi Gao, Zongping Liu, Haidong Li, Chengyin Wang
Summary: In this study, a new electrochemical biosensor for Pb2+ detection was designed based on the interactions between nucleic acids and nanomaterials. The biosensor showed easy preparation, adsorptive tunability, and high sensitivity, making it suitable for potential applications in food safety inspection and environmental monitoring.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Junhua Chen, Manjia Chen, Hui Tong, Fei Wu, Yizhang Liu, Chengshuai Liu
Summary: A fluorescence biosensor was developed to detect the available lead in soil samples with high sensitivity using DNAzyme and hairpin DNA cyclic assembly. The biosensor exhibited high selectivity and accuracy, and can be directly applied to the detection of available lead in soil samples.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Analytical
Zahra Khoshbin, Asma Verdian, Seyed Mohammad Taghdisi, Noor Mohammd Danesh, Khalil Abnous
Summary: In order to detect Pb2+ ions with high sensitivity and specificity, a simple optical biosensing array based on the cleavage activity of aptazyme strands and the regularity of liquid crystal molecules was introduced, which can detect ultra-low concentrations of Pb2+.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Yang Lu, Yiyue Yu, Yeru Wang, Wenbo Zhou, Ziyi Cheng, Liandong Yu, Shaojiang Zheng, Rongke Gao
Summary: In this study, a microfluidic chip based on SERS technology was developed for precise detection of miRNA. By utilizing MCASS as the SERS substrate and combining DNAzyme walker amplification technology with SERS nanoprobes, high sensitivity detection of miR-141 was achieved.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Applied
Jiafeng Pan, Zhi Liu, Junhua Chen
Summary: A biosensor for BPA detection was developed by integrating an amplifying DNA circuit with Mg2+-dependent DNAzyme in the sensing system, achieving a high sensitivity with a detection limit of 50 fM. The method proved to be selective and accurate, even working well in milk samples. The biosensor results were validated by LC-MS, showing no significant difference with the analytical method.
Article
Pharmacology & Pharmacy
Zunaira Shoaib, Timothy M. Fan, Joseph M. K. Irudayaraj
Summary: Osteosarcoma, a common primary bone tumor, is influenced by mechanobiology in its pathogenesis. Mechanobiology-driven therapies can curb tumor progression by altering the physical microenvironment or inhibiting proteins associated with metastasis.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Wenjie Liu, Abinash Padhi, Xiaohui Zhang, Jairaj Narendran, Mark A. Anastasio, Amrinder S. Nain, Joseph Irudayaraj
Summary: This study elucidates the spatiotemporal organization of heterochromatin in the elongated nuclei of cells on aligned nanofibers. The findings suggest that the anisotropy of nuclei is sufficient to drive heterochromatin alterations, and reveal an unconventional H3K9me3 heterochromatin distribution.
Article
Engineering, Biomedical
Yoon Jeong, Joseph Irudayaraj
Summary: In order to sequester prokaryotic cells in a biofilm-like niche, it is crucial to create a microenvironment that reflects the heterogeneous nature of biological systems. This study presents an artificial long-term confinement model using natural alginate hydrogels that can support the growth and survival of organisms for over 10 days. The confinement platform allows for the encapsulation of bacterial cells in both the core and shell layers, with tunable thickness and number of shell layers.
ACTA BIOMATERIALIA
(2023)
Article
Biophysics
Yoon Jeong, Wentao Kong, Ting Lu, Joseph Irudayaraj
Summary: In this study, a natural soft hydrogel bioactuator based on alginate core-shell structures was developed to enable 3D microbial colonization at high cell density. Compared to conventional techniques, this system showed a significant increase in cellular biomass, enzymatic activity, and bioluminescence signal, while providing sustained support for bacterial growth within confined space.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Kristina Rhee, Anastasiia Tukova, Mohammad Tavakkoli Yaraki, Yuling Wang
Summary: This paper presents a simple synthesis method to produce quasi-spherical SERS nanotags and larger highly anisotropic nanoparticles, which contain gold/silver nanostars, a self-assembled monolayer of Raman reporter molecules, and a silver coating. The Raman reporter molecule, 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB), is found to play a crucial role in controlling the morphology of the nanosupernova.
Article
Food Science & Technology
Zhiyang (Stan) Tu, Joseph Irudayaraj, Youngsoo Lee
Summary: This study aimed to enhance the understanding of applying NIR spectroscopy in spray-dried samples by comparing two sample preparation strategies and two spectrometers. The results showed that the sample preparation strategies had little impact on NIR calibration model development. Although the benchtop NIR spectrometer outperformed the portable NIR spectrometer, the portable NIR spectrometer still demonstrated reliable performance for moisture analysis.
Article
Chemistry, Physical
Nhung Thi Tuyet Nguyen, Mohammad Tavakkoli Yaraki, Yuling Wang
Summary: Surface-enhanced Raman scattering (SERS) is a non-invasive analytical technique with ultra-sensitivity and high multiplex capability. Gold nanostars (AuNSs) with sharp tips and corners, and a unique near-infrared localized surface plasmon resonance, have shown great potential in SERS and biomedical applications. In this study, we report the reproducible synthesis of AuNSs using a seedless, surfactant-free, and green method. The AuNSs' localized surface plasmon resonance (LSPR) was tuned, and seven types of SERS nanotags were prepared for spectral barcoding, resulting in 127 barcodes. This spectral barcoding approach opens a new avenue for future multiplex applications.
MOLECULAR SYSTEMS DESIGN & ENGINEERING
(2023)
Review
Environmental Sciences
Xing Zhang, Jodi A. Flaws, Michael J. Spinella, Joseph Irudayaraj
Summary: Endocrine disrupting chemicals (EDCs) are exogenous substances that can alter the endocrine function of an organism, leading to adverse effects on growth, development, metabolism, and reproductive function. Studies have shown that EDCs can cause proteinuria, affect glomeruli and renal tubules, and even lead to diabetes and renal fibrosis in both animals and humans. This review discusses the renal accumulation of specific EDCs, such as dioxins, per- and polyfluoroalkyl substances (PFAS), bisphenol A (BPA), and phthalates, and explores how exposure to these EDCs can result in renal lesions and diseases, including cancer. The regulation of target genes and activation of related pathways by typical EDCs are also summarized.
Review
Medicine, Research & Experimental
Xiaoxue Han, Leah Suyeon Ju, Joseph Irudayaraj
Summary: Oxygen is crucial in regulating various processes in wound healing. However, hypoxia commonly found in wounds can hinder normal healing. To enhance wound healing, oxygenation strategies such as incorporating oxygen delivery materials are effective. This review summarizes the stages of wound healing, the role of hypoxia, and current strategies for incorporating oxygen into wound dressings. The efficacy, mechanisms, and potential benefits and drawbacks of various oxygenation materials are discussed.
MOLECULAR PHARMACEUTICS
(2023)
Article
Environmental Sciences
Faizan Rashid, Veronika Dubinkina, Saeed Ahmad, Sergei Maslov, Joseph Maria Kumar Irudayaraj
Summary: Exposure to PFOS and GenX has different effects on the microbiome of the small intestine and colon, as well as on liver metabolism. High doses of GenX increase the abundance of certain bacteria, while PFOS generally alters the abundance of other bacteria. These treatments are associated with alterations in important microbial metabolic pathways. Additionally, PFOS and GenX exposure can cause metabolic disorders in the liver.
Article
Engineering, Environmental
Yoon Jeong, Khushali Vyas, Joseph Irudayaraj
Summary: This study investigates the toxicity of PFAS on bacteria in a biofilm-like niche provided by hydrogel-based core-shell beads. The findings show that Escherichia coli MG1655 strain confined in hydrogel beads exhibit altered physiological characteristics compared to their susceptible counterpart cultivated under planktonic conditions. The study suggests that soft-hydrogel engineering platforms may protect microorganisms from environmental contaminants.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Wen Ren, Victoria Messerschmidt, Michael Tsipursky, Joseph Irudayaraj
Summary: In this study, an oxygen nanobubbles-embedded hydrogel (ONB-G) with carbopol was proposed for oxygenation of wounds to accelerate wound healing. ONB-G, prepared by integrating carbopol, hydrogel, and dextran-based oxygen nanobubbles (ONBs), can hold and release oxygen to promote wound healing. Experimental results showed that ONB-G could encapsulate oxygen for up to 34 days and maintain high oxygen levels for up to 4 weeks. HDFa cell-based studies demonstrated the potential of ONB-G in enhancing cell viability, proliferation, and wound closure. Histological evaluation using a pig model also indicated improved healing with ONB-G treatment compared to control groups. Overall, dextran-shell ONBs embedded in a gel exhibit promising potential in accelerating wound healing.
ACS APPLIED NANO MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Anastasiia Tukova, Yihan Nie, Mohammad Tavakkoli Yaraki, Ngoc Thanh Tran, Jiaqiu Wang, Alison Rodger, Yuantong Gu, Yuling Wang
Summary: Gold nanoparticles (AuNPs) undergo changes upon contact with biological media, leading to the formation of protein corona (PC) around the particles. Understanding the mechanisms and functions of PC formation is important for utilizing its benefits and avoiding drawbacks. This study investigates the differences in PC formation on spherical and anisotropic AuNPs, revealing that protein does not fully cover the surface of anisotropic nanoparticles, providing potential binding sites for analytes while stabilizing the AuNPs.
Article
Chemistry, Multidisciplinary
Yoon Jeong, Joseph Irudayaraj
Summary: This study demonstrates the design and fabrication of finely tuned alginate core-shell capsules with controllable core and shell thickness at the micron scale. These capsules were successfully used to encapsulate bacteria while maintaining their morphology and mechanical stability.
CHEMICAL COMMUNICATIONS
(2022)
Article
Genetics & Heredity
Yi Wen, Faizan Rashid, Zeeshan Fazal, Ratnakar Singh, Michael J. Spinella, Joseph Irudayaraj
Summary: The study showed that PFOS accumulated in mouse kidneys in a dose-dependent manner, leading to significant increase in kidney injury markers, affecting transcription factors and epigenetic mechanisms. The decrease in global DNA methylation levels suggests potential implications of PFOS on kidney fibrosis and carcinogenesis.
ENVIRONMENTAL EPIGENETICS
(2022)
Review
Chemistry, Multidisciplinary
Dan He, Yawen Li, Fuwen Zhao, Yuze Lin
Summary: High trap density in organic solar cells leads to localized charge carriers and reduced carrier lifetime, limiting device efficiency. This feature article summarizes the recent advances of trap suppression by material design and device engineering.
CHEMICAL COMMUNICATIONS
(2024)
Review
Chemistry, Multidisciplinary
Olivier Monfort, Arshitha Madhusudhan, Martin Motola
Summary: This article presents the advantages of coupling photocatalysis and Fenton-based processes for environmental remediation and discusses their synergy. It critically examines the simultaneous triggering of these two processes and provides insights into research approaches. The study aims to enhance understanding of this complex process and highlight the potential integration of new catalysts in current wastewater treatment technology.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Manideepa Dhar, Chittaranjan Mishra, Avijit Das, Uttam Manna
Summary: An approach of 'polymerization of monomers in its aggregated form' has been introduced to tailor the water wettability of fibrous and porous substrates, achieving hydrophobicity to superhydrophobicity transition, as well as patterned wettability. This facile chemical method provides a durable coating with adjustable and patterned wettability for various potential applications.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Saswat Ranjan Bhoi, Chhanda Debnath, Shikha Gandhi
Summary: In this article, a novel catalytic reaction using Pd(0) and Bronsted acid is reported for the synthesis of biologically important imidazolidinone-fused N-heterocycles through redox-neutral intramolecular N-allylation of indoles and pyrroles with alkynes. This atom-economical method is applicable to a wide range of substrates and eliminates the need for leaving groups or oxidizing agents commonly used in traditional allylation reactions.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Longkun Chen, Mingshuai Zhang, Meichen Liu, Zhuoyuan Liu, Yuetong Qiu, Zhilai Zhang, Fuchao Yu, Jiuzhong Huang
Summary: In this study, an efficient Rh(III)-catalyzed selective mono- and dual-C-H bond functionalization/cyclization with iodonium ylide as the sole coupling partner was demonstrated. Fused benzodiazepine skeletons were obtained in excellent yields, providing an improved approach to dual C-H unsymmetrical functionalization.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Alice Kennett, Sven Epple, Gabriella van der Valk, Irene Georgiou, Evelyne Gout, Romain R. Vives, Angela J. Russell
Summary: This study presents the design and synthesis of sulfated disaccharide inhibitors based on IdoA(2S)-GlcNS(6S), which showed potent inhibition of Sulf-2. The results suggest that IdoA(2S)-GlcNS(6S) is the shortest fragment size required for effective inhibition of Sulfs, and a trisulfated disaccharide is identified as the minimal fragment size of heparan sulfate for effective endosulfatase inhibition.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Mauro Garbini, Andrea Brunetti, Riccardo Pedrazzani, Magda Monari, Massimo Marcaccio, Giulio Bertuzzi, Marco Bandini
Summary: The self-adaptability of galvanostatic electrolysis was found to be helpful in a multistage chemo- and diastereoselective electrochemically promoted cyclodimerization of chalcones. Through a series of reductive events, densely functionalized cyclopentanes with five contiguous stereocenters were obtained (25 examples, yields up to 95%, dr values up to >20:1). Further experimental and electrochemical investigations indicated the crucial role of dynamic kinetic resolution of the aldol intermediate in the reaction mechanism.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Dhananjay S. Nipate, Neha Meena, Prakash N. Swami, Krishnan Rangan, Anil Kumar
Summary: In this study, the synthesis of functionalized benzo[a]phenazines and indazolo[2,3-a]quinolines was achieved through Rh(III)-catalyzed oxidative annulation of 2-arylquinoxalines and 2-aryl-2H-indazoles with allyl alcohols. The method showed a wide substrate scope, excellent functional group tolerance, good to high yields of annulated products, and the capability for scaled-up synthesis. A tentative mechanism of the annulation reaction was proposed based on a preliminary mechanistic investigation.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Ruonan Zhang, Abudukadi Tudi, Xia Yang, Xuping Wang, Zhihua Yang, Shujuan Han, Shilie Pan
Summary: A new lead tellurium borate material with a unique fundamental building block [B4O10] was synthesized and found to exhibit high birefringence. The structure-property relationship was discussed using first-principles calculations.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Amresh Kumar Singh, Baishali Thakurta, Anupam Giri, Monalisa Pal
Summary: This study introduces three key concepts for the quick synthesis of large-scale high-quality 2D materials films and their utilization in device fabrication.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Reina Hagiwara, Shun Nishimura, Kosuke Okeyoshi
Summary: A copolymer-conjugated nanocatalytic system has been developed to improve electron transfer and enhance photoinduced H2 generation.
CHEMICAL COMMUNICATIONS
(2024)
