A practical solution-phase synthesis of an antagonistic peptide of TNF-alpha based on hydrophobic tag strategy

A simple acid-resistant hydrophobic tag, which can be removed rapidly in a single-step procedure after overall peptide synthesis, has been developed to accomplish practical solution-phase synthesis of a 15-mer antagonistic peptide of TNF-alpha (A-TNF-alpha). Hydrophobically tagged peptides can be separated as precipitates at each step by addition of a polar organic solvent.