期刊
CHEMICAL COMMUNICATIONS
卷 46, 期 40, 页码 7507-7509出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0cc02850a
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资金
- NIH [R01GM076360, U01CA128442, R01HD061935]
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT [R01HD061935] Funding Source: NIH RePORTER
- NATIONAL CANCER INSTITUTE [U01CA128442] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM076360] Funding Source: NIH RePORTER
A novel D-galactosyl-beta 1-3-N-acetyl-D-hexosamine phosphorylase cloned from Bifidobacterium infantis (BiGalHexNAcP) was used with a recombinant E. coli K-12 galactokinase (GalK) for efficient one-pot two-enzyme synthesis of T-antigens, galacto-N-biose (Gal beta 1-3GalNAc), lacto-N-biose (Gal beta 1-3GlcNAc), and their derivatives.
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