Review
Infectious Diseases
Gabriela Silva Cruz, Ariane Teixeira dos Santos, Erika Helena Salles de Brito, Gandhi Radis-Baptista
Summary: This review presents the antibacterial, antiparasitic, and antiviral effects of cell-penetrating antimicrobial peptides and highlights their potential for treating intracellular infections.
Article
Biochemistry & Molecular Biology
Keisuke Tsuchiya, Masato Kiyoshi, Noritaka Hashii, Minami Fujita, Takashi Kurohara, Akiko Ishii-Watabe, Kiyoshi Fukuhara, Takashi Misawa, Yosuke Demizu
Summary: The Wnt/β-catenin pathway has been implicated in the development and progression of various cancers. In this study, the researchers focused on the interaction between β-catenin and LRH-1 and developed a series of LRH-1-derived peptides, including Penetratin-st7, as potential inhibitors of Wnt signaling. The results showed that Penetratin-st7 effectively inhibited cell growth by targeting the Wnt pathway.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Gyula Batta, Levente Karpati, Gabriela Fulaneto Henrique, Gabriella Toth, Szabolcs Tarapcsak, Tamas Kovacs, Florina Zakany, Istvan M. Mandity, Peter Nagy
Summary: Cell penetrating peptides can be affected by the biophysical properties of the cell membrane. Treatment with atorvastatin can significantly increase the endosomal escape of penetratin.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Pramod Shah, Chien-Sheng Chen
Summary: Cell-penetrating peptides (CPPs) are peptides that can translocate across the cell envelope and have been used as vehicles for drug delivery. In this study, the potential protein targets of Penetratin, a well-known CPP, were screened using high-throughput proteome microarrays. The results showed that Penetratin targets proteins involved in ribonucleoprotein complex biogenesis, nucleic acid metabolic processes, and protein-protein interactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Miray Tonk, James J. Valdes, Alejandro Cabezas-Cruz, Andreas Vilcinskas
Summary: Arthropod antimicrobial peptides (AMPs) provide a potential source for new antibiotics with potent activity against multidrug-resistant bacterial pathogens. Synergistic effects can be achieved by combining AMPs from different sources. Hybrid peptides linked by glycine spacers show enhanced antibacterial activity, but can also exhibit increased cytotoxic activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Gina Barkowsky, Corina Abt, Irina Poehner, Adam Bieda, Sven Hammerschmidt, Anette Jacob, Bernd Kreikemeyer, Nadja Patenge
Summary: Streptococcus pneumoniae is a common cause of community-acquired pneumonia and other infectious diseases, and antibiotic resistance and strain replacement are growing problems. In this study, CPP-coupled antisense PNAs showed antimicrobial activity against S. pneumoniae, reducing the viability of the bacteria in vitro and increasing the survival rate of infected larvae in vivo. These findings suggest the potential development of efficient antisense PNAs for the treatment of S. pneumoniae infections.
MICROBIOLOGY SPECTRUM
(2022)
Article
Pharmacology & Pharmacy
Dagmar Yr orgeirsdottir, Jeppe Hofman Andersen, Marcus Perch-Nielsen, Laura Hyrup Moller, Freja Gronbaek-Thorsen, Hannah Gronbech Kolberg, Bente Gammelgaard, Mie Kristensen
Summary: The study compared the effect of labelling Tat and penetratin with 5(6)-carboxytetramethylrhodamine (TAMRA) and selenomethionine (MSe) on their physicochemical properties and on brain capillary endothelial cell (BCEC) models. TAMRA labelling increased the adherence and internalization of the peptides in BCECs compared to MSe labelling. However, TAMRA labelling of penetratin had a higher toxicity effect on the BCECs compared to TAMRA labelling of Tat, while MSe labelling did not affect cellular viability. Both TAMRA and MSe labelling decreased the barrier integrity of BCEC monolayers, but did not improve transport of the paracellular marker 14C-mannitol. Therefore, MSe labelling is preferred over TAMRA for quantification of peptides due to minimal alterations in physicochemical properties and effects on BCECs.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Noriyasu Kamei, Susumu Suwabe, Kenji Arime, Hidemi Bando, Kaho Murata, Maika Yamaguchi, Natsuki Yokoyama, Erina Tanaka, Ayaka Hashimoto, Takanori Kanazawa, Yukio Ago, Mariko Takeda-Morishita
Summary: This study investigated the mechanisms of nose-to-brain drug delivery enhancement by coadministering drugs with L-penetratin, demonstrating that it facilitates direct transport to specific brain regions and potentially allows targeting of certain brain areas.
Article
Chemistry, Medicinal
Maria Angeles Abengozar, Maria Fernandez-Reyes, Vivian A. Salazar, Marc Torrent, Beatriz G. de la Torre, David Andreu, Ester Boix, Luis Rivas
Summary: Eosinophil cationic protein (ECP) can enter the cytoplasm of Leishmania parasites, leading to RNA degradation and playing a key role in parasite killing. ECP combines membrane destabilization and enzymatic activities to effect parasite killing.
ACS INFECTIOUS DISEASES
(2022)
Article
Chemistry, Medicinal
Maria Angeles Abengozar, Maria Fernandez-Reyes, Vivian A. Salazar, Marc Torrent, Beatriz G. de la Torre, David Andreu, Ester Boix, Luis Rivas
Summary: The research suggests that eosinophils recruitment into Leishmania lesions may contribute to treatment. Eosinophil cationic protein (ECP) shows broad antimicrobial activity and enters the Leishmania cytoplasm leading to parasite killing through mechanisms such as RNA degradation.
ACS INFECTIOUS DISEASES
(2022)
Article
Microbiology
Huajie Zhu, Cuihong Xu, Yicun Chen, Yan Liang
Summary: In this study, antifungal compounds produced by Burkholderia arboris were identified using genetic and metabolomic approaches. The cobA gene was found to contribute to the antifungal activity of B. arboris. The synthesized Hafk peptide displayed significant antifungal activity against plant pathogens, suggesting its potential for biocontrol of fungal diseases in plants.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Engineering, Biomedical
Qi Tang, Peng Tan, Zhaolai Dai, Tao Wang, Shenrui Xu, Yakun Ding, Junqi Jin, Xin Zhang, Yucheng Zhang, Chenlong Zhou, Zitian Yue, Huiyang Fu, Junshu Yan, Xi Ma
Summary: This study investigates the association between hydrophobicity and cell-penetrating peptide delivery efficiency, and successfully develops chimeric peptides P3I7 and P3L7 with strong antibacterial activity and low cytotoxicity. These peptides can effectively penetrate cells to eliminate intracellular pathogens mainly through endocytosis. The mechanism of membrane destruction makes them fast sterilizers and less prone to drug resistance. In vivo trials confirm their good biocompatibility and antibacterial infection effects, indicating their potential as affordable and effective antimicrobial agents against intracellular bacterial infections.
ACTA BIOMATERIALIA
(2023)
Article
Fisheries
Daniel E. Saenz-Martinez, Paula A. Santana, Monica Arostica, Juan C. Forero, Fanny Guzman, Luis Mercado
Summary: The C-terminal end of IL-8 can be cleaved under acidic conditions, retaining its antibacterial properties and detectable in tissues of infected rainbow trout.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Mingcong Niu, Xiulian Gu, Jingyi Yang, Haoyu Cui, Xinyi Hou, Yue Ma, Chunhua Wang, Guangcheng Wei
Summary: Bacterial drug resistance poses a serious threat, necessitating the development of effective antibacterial drugs. This study focused on the design and synthesis of glycolipidpeptides with excellent antibacterial and immunomodulatory activities, which exhibited sustained-release properties and combined sterilization effects. The results demonstrate the potential applications of this research.
Article
Chemistry, Multidisciplinary
Mingcong Niu, Xiulian Gu, Jingyi Yang, Haoyu Cui, Xinyi Hou, Yue Ma, Chunhua Wang, Guangcheng Wei
Summary: Bacterial drug resistance is a serious issue, and the development of effective antibacterial drugs is urgently needed. Antimicrobial peptides (AMPs) show great potential as candidates for fighting bacteria. In this study, a series of AMPs with specific biological characteristics were designed and synthesized. Among them, the glycolipidpeptide GLP6 demonstrated excellent antibacterial and immunomodulatory activity, stability, biocompatibility, biofilm eradication, and membrane penetrating activity.
Article
Oncology
Kanjoormana Aryan Manu, Tin Fan Chai, Jing Tsong Teh, Wan Long Zhu, Patrick J. Casey, Mei Wang
MOLECULAR CANCER THERAPEUTICS
(2017)
Article
Biochemistry & Molecular Biology
Jingxian Liu, Wanlong Zhu, Haina Qin, Jianxing Song
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2009)
Article
Biochemistry & Molecular Biology
Wan Long Zhu, Mohammed S. Hossain, Dian Yan Guo, Sen Liu, Honglian Tong, Atefeh Khakpoor, Patrick J. Casey, Mei Wang
JOURNAL OF BIOLOGICAL CHEMISTRY
(2011)
Article
Biochemistry & Molecular Biology
Wan Long Zhu, Song Yub Shin
JOURNAL OF PEPTIDE SCIENCE
(2009)
Article
Biochemistry & Molecular Biology
Wan Long Zhu, Kyung-Soo Hahm, Song Yub Shin
JOURNAL OF PEPTIDE SCIENCE
(2009)
Article
Biochemistry & Molecular Biology
J. T. Teh, W. L. Zhu, O. R. Ilkayeva, Y. Li, J. Gooding, P. J. Casey, S. A. Summers, C. B. Newgard, M. Wang
Article
Multidisciplinary Sciences
Xuelu Huan, Jiahai Shi, Liangzhong Lim, Sayantan Mitra, Wanlong Zhu, Haina Qin, Elena B. Pasquale, Jianxing Song
Article
Multidisciplinary Sciences
Wan Long Zhu, Honglian Tong, Jing Tsong Teh, Mei Wang
Article
Oncology
Jing Tsong Teh, Wan Long Zhu, Christopher B. Newgard, Patrick J. Casey, Mei Wang
MOLECULAR CANCER THERAPEUTICS
(2019)
Article
Biochemistry & Molecular Biology
Wan Long Zhu, Kyling-Soo Hahm, Song Yub Shin
JOURNAL OF PEPTIDE SCIENCE
(2007)
Article
Biochemistry & Molecular Biology
Sung-Ah Lee, Yu Kyoung Kim, Shin Saeng Lim, Wan Long Zhu, Hyunsook Ko, Song Yub Shin, Kyung-Soo Hahm, Yangmee Kim
Article
Biochemistry & Molecular Biology
Wan Long Zhu, Hongliang Lan, Yoonkyung Park, Sung-Tae Yang, Jae Il Kim, Il-Seon Park, Ho Jin You, Jung Sup Lee, Yong Sun Park, Yangmee Kim, Kyung-Soo Hahm, Song Yub Shin
Article
Biochemistry & Molecular Biology
Wan Long Zhu, Yun Mi Song, Yoonkyung Park, Ka Hyon Park, Sung-Tae Yang, Jae Il Kim, Il-Seon Park, Kyung-Soo Hahm, Song Yub Shin
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2007)
Article
Biochemistry & Molecular Biology
Ju-Un Lee, Dong-Il Kang, Wan Long Zhu, Song Yub Shin, Kyung-Soo Hahm, Yangmee Kim
Article
Biochemistry & Molecular Biology
Ali Mehri, Karim Mahnam, Hajar Sirous, Mahmoud Aghaei, Leila Rafiei, Mahboubeh Rostami
Summary: One potential approach for tumor therapy is inhibiting the binding between MDM2 and p53 to reactivate p53 in tumor cells. In this study, Monastrol derivatives were designed as MDM2 inhibitors and evaluated for their cytotoxicity on cancer cells. Compound 5d showed the best inhibitory results in silico and in vitro experiments. These findings suggest that Monastrol derivatives have the potential to be candidates for MDM2 inhibition.
CHEMICAL BIOLOGY & DRUG DESIGN
(2024)