Article
Biochemistry & Molecular Biology
Marta Peirats-Llobet, Changyu Yi, Lim Chee Liew, Oliver Berkowitz, Reena Narsai, Mathew G. Lewsey, James Whelan
Summary: A spatial transcriptomics workflow was developed for studying gene expression during barley grain germination, revealing specific spatial expression patterns of functional gene categories. Over 14,000 genes were differentially regulated within the first 24 hours after imbibition, with certain genes showing focused expression within subdomains of the embryo over time, suggesting their role in establishing the embryo axis.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Xiaowei Gao, Bhagyashree N. Swarge, Winfried Roseboom, Peter Setlow, Stanley Brul, Gertjan Kramer
Summary: In this study, the proteome of B. cereus spores during germination and outgrowth was investigated using mass spectrometry and co-expression network analysis. A unique global proteome view of these processes was obtained, and 2211 proteins were monitored for changes. The study clustered the germination proteome data into eight modules and identified 124 proteins that could be vital during spore revival in B. cereus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
J. Liu, L. Yang, Y. Yuan, T. Xue
Summary: Water uptake is essential for seed germination, and the endocarp of pecan seed plays an important role in this process. Various techniques were used to analyze the spatiotemporal pattern of water uptake and the effect of the endocarp on water absorption. Isolated seeds completed water uptake in 8 hours, while whole seeds required 6 days, indicating the significance of cracking the endocarp. The hilum serves as the entry point for water, while the rest of the seed coat acts as a barrier due to the waxy layer. The U-shaped region of the pecan seed has the highest water content, and water gradually diffuses from this region to the entire kernel. A new stage of water absorption was observed between phase II and phase III of the triphasic model of water uptake in pecan seeds. Cracking the endocarp changes water distribution and may trigger further water absorption and radicle elongation.
Editorial Material
Biochemistry & Molecular Biology
Bailey Crowley, Avery Russell
Summary: Microbial residents of floral nectar rely on pollen in nectar for nutrients and growth.
Article
Multidisciplinary Sciences
Mariusz A. Pietruszka
Summary: Through the study of tobacco and hyacinth pollen tubes, it was found that time series measurements and numerical calculations using information theory methods represent signatures of collective dynamics in living cells at critical-molecularly encoded-germination and growth temperatures. This method, along with soil pH data, can facilitate assisted plant migrations from one ecosystem to another as the Earth faces climate change.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Luisa Maria Diele-Viegas, Thamara Santos de Almeida, Iris Amati-Martins, Christine D. Bacon, Cibele Cassia-Silva, Rosane G. Collevatti, Jessica Fenker, Tabata Elise Ferreira Cordeiro, Giuliana Caldeira Pires Ferrari, Ana Clara Sampaio Franco, Luiza Flores Gasparetto, Juliana Hipolito, Camila Hohlenwerger, Beatriz Hormanseder, Priscila Barreto de Jesus, Suzana dos Santos Matos, Daniela Pareja-Mejia, Beatriz Moraes Murer, Carla Brunner Pavone, Flavia B. Pilecco, Caren Queiroz-Souza, Alice Reis, Pamela Cristina Santana, Fernanda Dias-Silva, Lucy Souza, Mariana P. C. Telles, Jemilli Viaggi, Flavia Virginio
Summary: Understanding gaps in academic representation and considering intersectionality are crucial for promoting inclusive progress in STEM.
NATURE COMMUNICATIONS
(2022)
Article
Food Science & Technology
Xue Dong, Litao Sun, Manjree Agarwal, Garth Maker, Yitao Han, Xiangyang Yu, Yonglin Ren
Summary: In this study, after-ripening and ozone treatment were used to promote germination of barley seeds, and the metabolic changes during barley germination were revealed. These findings have important implications for understanding the mechanism of seed dormancy and crop growth.
Article
Environmental Sciences
Li Zhang, Jiaxin Ma, Peipei Liu, Anxiang Qi, Haiyan Jin, Renming Jia, Zhenming Zheng, Cheng Yan, Minghong Cai
Summary: With the prohibition of conventional organochlorine pesticides, the use of current-use pesticides such as organic amine pesticides and organophosphate pesticides in agriculture and mariculture activities has become a global concern. A study in the Beibu Gulf, a subtropical agricultural and maricultural zone, found that pesticides were widely present in seawater and sediment, with the most abundant categories being organic amine pesticides and organophosphate pesticides. The spatial distribution of pesticides showed a decrease from coastal areas to the sea, indicating the impact of anthropogenic activities on pesticides pollution.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Plant Sciences
Zhenye Li, Jun Fu, Zhi Chen, Qiankun Fu, Xiwen Luo
Summary: This study proposes a method for detecting peeling-damaged fresh corn using hyperspectral imaging. The germinating sparse classification (GSC) method and the threshold sparse recovery algorithm are used to achieve pixel-level classification, resulting in good classification accuracy.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Baojian Chen, Martijn Fiers, Bas J. W. Dekkers, Lena Maas, G. Wilma van Esse, Gerco C. Angenent, Yang Zhao, Kim Boutilier
Summary: Research shows that exposing explants to growth regulator 2,4-D can induce somatic embryogenesis in Arabidopsis seeds, with a crucial role played by ABA in promoting this process by maintaining the seed maturation environment and regulating the expression of transcription factors.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Chemistry, Multidisciplinary
Juan Tang, Liping Liu, Shan Gao, Jiao Qin, Xiaoxuan Liu, Dianping Tang
Summary: A simple thermal aptasensing platform was developed for sensitive detection of organophosphate pesticides, specifically using malathion as a model target. The platform utilizes the efficient self-heating reaction of a warming pad with a switchable target responsive enzyme-encapsulated three-dimensional DNA hydrogel, with a portable thermometer as the signal readout. This strategy demonstrates excellent specificity, reproducibility, and stability, and has potential for extension to monitor other molecules using different aptamer sequences.
CHEMICAL COMMUNICATIONS
(2021)
Article
Plant Sciences
Lindsay C. DeMers, Victor Raboy, Song Li, M. A. Saghai Maroof
Summary: The low phytic acid trait in soybeans is conferred by loss-of-function mutations in specific genes, leading to potential negative effects on seed vigor. However, the benefits of this trait outweigh the drawbacks, prompting the need for a deeper understanding of the molecular basis behind the negative effects. Transcriptomic data analysis reveals that lpa seeds have increased stress sensitivity due to disruptions in various metabolic processes, potentially affecting seed germination. This study contributes valuable insights into the regulatory elements governing seed viability and germination in lpa soybeans.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Chemistry, Analytical
Peng Wang, Xinxin Xu, Lingling Guo, Liqiang Liu, Hua Kuang, Jing Xiao, Chuanlai Xu
Summary: In this study, two haptens similar in structure to nitrofen (NIT) were synthesized, and five monoclonal antibodies capable of recognizing NIT and bifenox (BIF) were screened out by competitive ELISA, with the lowest IC50 values of 0.87 ng mL(-1) and 0.86 ng mL(-1), respectively. The antibody 5G7 was combined with colloidal gold to establish a lateral flow immunochromatographic assay strip. This method could qualitatively and quantitatively detect the residues of NIT and BIF in fruit samples. The strip assay could be used for rapid analysis of fruit samples.
Review
Gastroenterology & Hepatology
Tusha Sharma, Nagabhishek Sirpu Natesh, Ramesh Pothuraju, Surinder K. Batra, Satyanarayana Rachagani
Summary: The human gut microbiota can be disrupted by environmental contaminants such as pesticides, posing potential health risks. The metabolites produced by gut microbiota play a significant role in maintaining host health, and an imbalance in this system can lead to various diseases. Recent studies have shown that pesticides have harmful effects on the host's gut microbiota. Therefore, further research is needed to understand the impact of pesticides on gut microbiota-mediated metabolic changes, which may provide insights into pesticide-induced toxicity.
Article
Biochemistry & Molecular Biology
Maria Breygina, Alexander Voronkov, Tatiana Ivanova, Ksenia Babushkina
Summary: Pollen grains are unique haploid organisms with special composition and structure. Angiosperms and gymnosperms share similar mechanisms for pollen germination but gymnosperms exhibit slower growth rates and reduced reliance on female tissues. The properties of pollen lipids, especially very-long-chain FAs, contribute to these features. By analyzing the absolute content and FA composition of pollen lipids, significant differences were found between species, with spruce pollen exhibiting a higher proportion of saturated and monoene FAs. Lily and tobacco also showed variations in the FA composition between pollen coat and gametophyte cells, with a lower unsaturation index in the coat. Moreover, the absolute content of lipids was highest in lily pollen. This study provides new insights into the FA changes during pollen germination in gymnosperms and angiosperms, and highlights the impact of H2O2 on spruce pollen germination.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
