期刊
JOURNAL OF FORENSIC SCIENCES
卷 60, 期 5, 页码 1234-1240出版社
WILEY
DOI: 10.1111/1556-4029.12828
关键词
forensic science; forensic anthropology; postmortem interval; human microbiome; gut bacteria; microbial ecology; quantitative polymerase chain reaction
资金
- William M. Bass Endowment
- University of Tennessee Undergraduate Summer Internship program
- Microbiology Across Campuses Research and Educational Venture
Postmortem succession of human-associated microbial communities (human microbiome) has been suggested as a possible method for estimating postmortem interval (PMI) for forensic analyses. Here we evaluate human gut bacterial populations to determine quantifiable, time-dependent changes postmortem. Gut microflora were repeatedly sampled from the proximal large intestine of 12 deceased human individuals as they decayed under environmental conditions. Three intestinal bacterial genera were quantified by quantitative PCR (qPCR) using group-specific primers targeting 16S rRNA genes. Bacteroides and Lactobacillus relative abundances declined exponentially with increasing PMI at rates of N-t=0.977e(-0.0144t) (r(2)=0.537, p<0.001) and N-t=0.019e(-0.0087t) (r(2)=0.396, p<0.001), respectively, where N-t is relative abundance at time (t) in cumulative degree hours. Bifidobacterium relative abundances did not change significantly: N-t=0.003e(-0.002t) (r(2)=0.033, p=0.284). Therefore, Bacteroides and Lactobacillus abundances could be used as quantitative indicators of PMI.
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