Article
Biochemistry & Molecular Biology
Zhengxun Wei, Sihong Wu, Jiujie Xia, Ping Shao, Peilong Sun, Ning Xiang
Summary: This research found that amyloid fibrils formed from hen egg-white lysozyme (HEWL) exhibited significantly enhanced antibacterial activity against both Staphylococcus aureus and Escherichia coli, while oligomers did not show improvement compared to native HEWL. These natural and inexpensive amyloid fibrils have potential applications in antimicrobial food packaging, animal feed, antibiotic replacement, etc.
Article
Biochemistry & Molecular Biology
Husnul Fuad Zein, Thana Sutthibutpong
Summary: Atomistic molecular dynamics simulations were used to study the effects of charged residues and pH on amyloid models. The simulations revealed that antiparallel configurations had greater stability and compactness compared to parallel configurations. The study also provided insights into the importance of specific residues and the disruption of interaction networks in amyloid formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Multidisciplinary
Pingfan Wu, Yu Wang, Bo Huang, Zicheng Xiao
Summary: Anderson-type polyoxometalates have made significant progress in the past decade and have shown unique charm in multiple fields. Their highly flexible topological structure and diverse functionalization methods make them ideal platforms for rational design and controllable synthesis.
Article
Nanoscience & Nanotechnology
Srijita Paul, Titas Kumar Mukhopadhyay, Sandip Paul
Summary: Inspired by the urge to determine the underlying mechanism of toxic effects induced by two-dimensional nanomaterials, this study investigates the interactions between graphene, h-BN, and a model protein HEWL. It is found that the protein can be easily adsorbed on both graphene and h-BN, but h-BN exhibits a stronger adsorption affinity. Aromatic and aliphatic residues play a significant role in the adsorption process. The disruption of intraprotein hydrogen bonds by h-BN leads to changes in the protein's secondary structure. The study highlights the importance of understanding nanotoxicity for the design of biocompatible 2D materials.
ACS APPLIED NANO MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Prakriti Seth, Aniket Mukherjee, Nandini Sarkar
Summary: This research focuses on developing functional amyloid-based hydrogels as drug delivery tools and analyzes their potential in the biomedical sector. Three types of amyloid-based hydrogels were synthesized and characterized, with variations observed based on compositional differences. Drug release assays were conducted to explore their potential as drug delivery tools.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Mohammed J. Hakeem, Javed Masood Khan, Ajamaluddin Malik, Fohad Mabood Husain, Vivek Ambastha
Summary: Several food dyes, including sunset yellow, can induce amyloid fibrillation in proteins such as hen egg white lysozyme (HEWL). This study found that sunset yellow dye induced fast aggregation in HEWL without a lag phase, following an isodesmic pathway. The aggregates had a cross-beta secondary structure and were affected by salts and solvents. The results suggest that electrostatic and hydrophobic interactions play a role in sunset yellow-induced amyloid fibrillation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Physical
Sona Lyndem, Rabiul Gazi, Vinay Kumar Belwal, Anindita Bhatta, Madhurima Jana, Atanu Singha Roy
Summary: In this study, multi-spectroscopic and computational methods were used to investigate the binding mechanism of esculin and esculetin with hen egg white lysozyme (HEWL). The results showed that both compounds interacted with HEWL through a static quenching mechanism with high binding constants. Hydrophobic forces and hydrogen bonding were involved in the binding process, leading to conformational changes and altered enzyme activity of HEWL.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Pharmacology & Pharmacy
Hani A. Alhadrami, Ahmed M. Sayed, Hossam M. Hassan, Mostafa E. Rateb, Karim Abdelkader
Summary: This study presents a safe and effective approach utilizing cecropin A to facilitate hen egg white lysozyme access to peptidoglycan layers. The combination of HEWL and cecropin A shows potential antibacterial activity against Salmonella enterica.
Article
Multidisciplinary Sciences
Javed Masood Khan, Ajamaluddin Malik, Mohammad Z. Ahmed
Summary: This study investigated the interaction between CHAPS and HEWL using various biophysical techniques, showing that CHAPS strongly interacts with HEWL, affecting protein conformations by inducing secondary structure and disrupting tertiary structure. The results provide detailed insights into the molecular-level interaction of CHAPS with HEWL.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Sauradipta Banerjee
Summary: The study found that N-benzylisatin has a inhibitory effect on stress-induced aggregation of HEWL, enhancing the conformational stability of HEWL.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
Lin Cui, Huoqing Huang, Honglian Zhang, Xiaolu Wang, Xing Qin, Tao Tu, Jie Zhang, Xiaoyun Su, Huimin Yu, Yingguo Bai, Huiying Luo, Bin Yao, Yuan Wang
Summary: A novel fusion protein expression strategy was proposed to increase the expression level of hen egg white lysozyme (HEWL). The results showed that the fusion protein expressed using this strategy had higher expression level and activity compared to HEWL alone.
Article
Biochemistry & Molecular Biology
Xuejiao Gao, Sujuan Wang, Jiawei Dong, Jie Li, Yuangong Zhang, Yuxia Wu, Xinwu Ba
Summary: This study investigated the inhibitory effect of a new mono-ketone-containing curcumin analogue (MDHC) on amyloid fibrillation of hen egg white lysozyme (HEWL). Although MDHC had a lower inhibitory capacity compared to curcumin, it still demonstrated significant inhibitory effect. The findings indicated that the beta-diketone moiety played an important role in the inhibition process of HEWL amyloid fibrillation.
BIOPHYSICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Sona Lyndem, Rabiul Gazi, Madhurima Jana, Vinay Kumar Belwal, Atanu Singha Roy
Summary: The study utilized multispectroscopic and computational methods to explore the interaction between two coumarin derivatives and a carrier protein, revealing moderate binding affinity and the dominant role of hydrophobic forces. Molecular dynamics simulation indicated structural stability gained by the carrier protein upon interaction with the compounds, suggesting a change in its activity.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biochemistry & Molecular Biology
Fredrick Nwude Eze, Roseline Chika Eze, Chitchamai Ovatlarnporn
Summary: The research found that biogenic gold nanoparticles can inhibit protein amyloidogenesis, which is linked to chronic diseases such as Alzheimer's and Parkinson's disease. The synthesized nanoparticles, stabilized with quercetin-functionalized tara gum, demonstrated strong anti-aggregation and fibrillation effects against amyloidogenic proteins. They also showed potential for countering protein aggregation and associated disease conditions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Physical
Samal Kaumbekova, Dhawal Shah
Summary: Deep eutectic solvents (DESs) are considered as environmentally friendly alternatives to ionic liquids. Experimental and molecular dynamics (MD) simulation studies have suggested that DESs and their aqueous solutions can preserve protein structure and enhance thermal stability, with the potential for partial refolding at room temperature.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Review
Chemistry, Inorganic & Nuclear
Manuel Aureliano, Nadiia I. Gumerova, Giuseppe Sciortino, Eugenio Garribba, Craig C. McLauchlan, Annette Rompel, Debbie C. Crans
Summary: This review provides a detailed description of the interactions between polyoxidovanadates (POVs) and various proteins, as well as their pharmacological actions. X-ray crystallographic studies were used to investigate the interactions between POVs and specific proteins. Additionally, the interactions of POVs with proteins related to muscle contraction, serum, oxidative stress, and diabetes were discussed. The modes of interaction for POVs include H-bond, electrostatic, H-bond + electrostatic, van der Waals, and covalent binding.
COORDINATION CHEMISTRY REVIEWS
(2022)
Article
Chemistry, Multidisciplinary
Emir Al-Sayed, Elias Tanuhadi, Gerald Giester, Annette Rompel
Summary: In this study, the hybridization of lanthanide-containing molybdenum blue (Ln-MB) with 2-picolinic acid was achieved, resulting in the formation of Ln-MB molecules with a unique shape. The position of the carboxylic acid group was found to be crucial in obtaining single crystals of Ln-MB. Various experimental techniques were employed to confirm the structure and composition of the synthesized molecules.
ACTA CRYSTALLOGRAPHICA SECTION C-STRUCTURAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Ian Oesterle, Dominik Braun, Annette Rompel, Benedikt Warth
Summary: Establishing a targeted LC-MS/MS method for human biomonitoring of polyphenols allows for accurate quantification of various polyphenol classes and high-throughput sample processing in large-scale population studies. The method demonstrates high extraction efficiency and low detection limits across multiple sample types. In a validation and proof-of-principle study, the method successfully detected and studied the concentration changes of multiple polyphenols in urine after ingestion of a high polyphenol smoothie.
ANALYTICA CHIMICA ACTA
(2022)
Article
Plant Sciences
Maja Mikulic-Petkovsek, Anton Ivancic, Sasa Gacnik, Robert Veberic, Metka Hudina, Silvija Marinovic, Christian Molitor, Heidi Halbwirth
Summary: This study analyzed the changes in sugar content, organic acids, phenolic compounds, and selected enzyme activities in the anthocyanin pathway in NIGRA (Sambucus nigra var. nigra-black fruits) and VIRIDIS (S. nigra var. viridis-green fruits) fruits at four stages of ripening. The results showed that glucose and fructose content in green fruits were higher than in colored fruits, and sugar content increased significantly until the third developmental stage. NIGRA berries had a high content of anthocyanins and flavonols, while flavonols were the major phenolic group in VIRIDIS fruits. NIGRA fruits showed strong activation of the late anthocyanin pathway, while VIRIDIS fruits lacked anthocyanins and only had one flavanol.
Article
Chemistry, Inorganic & Nuclear
Morteza Rafieeshad, Nadiia I. Gumerova, Elias Tanuhadi, Gerald Giester, Hana Cipcic-Paljetak, Donatella Verbanac, Annette Rompel
Summary: Four new Ni-substituted Krebs-type sandwich-tungstobismuthates decorated with amino acids have been synthesized by one-pot solution methods. Only one compound showed antibacterial activity against four bacterial strains with minimum inhibitory concentration ranging from 8 to 256 μg/mL.
INORGANIC CHEMISTRY
(2023)
Editorial Material
Materials Science, Multidisciplinary
Manuel Aureliano, Nadiia I. Gumerova, Annette Rompel
Article
Multidisciplinary Sciences
Nadiia I. Gumerova, Annette Rompel
Summary: This article introduces a method for studying the coordination phenomena of complex polyatomic ions and develops a speciation atlas containing 10 commonly used polyoxometalates. The atlas provides a database and predictive model for other polyoxometalates, revealing the potential of polyoxometalates in biological and catalytic applications.
Article
Chemistry, Analytical
Ian Oesterle, Manuel Pristner, Sabrina Berger, Mingxun Wang, Vinicius Verri Hernandes, Annette Rompel, Benedikt Warth
Summary: Polyphenols, abundant in plants and fungi, have beneficial bioactive properties. High-resolution mass spectrometry (HRMS) is favored for analyzing their complexity. This study evaluated the advantages of HRMS, using untargeted techniques and online resources, for annotating polyphenols and other molecules. Despite the sensitivity loss compared to low-resolution mass spectrometry (LRMS), HRMS is capable of comprehensively investigating human polyphenol exposure, linking it to human health effects and toxicological mixture effects with other xenobiotics.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Claudia Iriarte-Mesa, Matthias Pretzler, Cornelia von Baeckmann, Hanspeter Kahlig, Regina Kaehlig, Annette Rompel, Freddy Kleitz
Summary: This study demonstrates the efficient catalytic oxidation of various phenolic compounds by immobilizing functional mushroom tyrosinase on mesoporous SBA-15-type silica, while retaining the enzyme activity. This method shows potential for in situ identification of soil samples.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Review
Chemistry, Multidisciplinary
Ronald Stebegg, Georg Schmetterer, Annette Rompel
Summary: This review highlights that cyanobacteria are more than simple photoautotrophs and their flexibility towards different environmental conditions has been underestimated in the past. It provides a summary of known cyanobacterial strains capable of heterotrophy and lists the potential substrates for each strain. The review discusses the specific conditions that induce heterotrophic growth for each strain.
Article
Chemistry, Physical
Sreejith P. Nandan, Nadiia I. Gumerova, Jasmin S. Schubert, Hikaru Saito, Annette Rompel, Alexey Cherevan, Dominik Eder
Summary: This study successfully heterogenized a molecular all-inorganic polyoxometalate catalyst onto a TiO2 surface, creating a novel heterogeneous photosystem for efficient and stable light-driven water oxidation. The hybrid photosystem exhibited constant catalytic rate for at least 10 hours, demonstrating the stability provided by TiO2 and its role as a photosensitizer.
Article
Chemistry, Multidisciplinary
Elias Tanuhadi, Annette Rompel
Summary: Three new trisubstituted 4f- and 4d tungstoantimonates were synthesized using a double-template synthetic approach. The compounds were characterized in the solid state and in solution, and shown to be highly active, recyclable, and stable Lewis-acid catalysts for the hydrolysis of the nerve agent simulant DMNP.
CHEMICAL COMMUNICATIONS
(2022)
Review
Nanoscience & Nanotechnology
Emir Al-Sayed, Annette Rompel
Summary: This review discusses the classification, structural characteristics, and interactions with lanthanide ions of molybdenum blues. The focus is on wheel-shaped structures of molybdenum blues, including both organic and inorganic hybrid structures, and highlights the key factors and research methods in their synthesis. The unique behavior and potential applications of wheel-shaped Ln-MBs in catalysis, molecular recognition, and separation are emphasized.
ACS NANOSCIENCE AU
(2022)
Article
Food Science & Technology
Ala Eddine Derardja, Matthias Pretzler, Ioannis Kampatsikas, Milena Radovic, Anna Fabisikova, Martin Zehl, Malika Barkat, Annette Rompel
Summary: In this study, the effect of enzymatic browning on the phenolic composition of apricot was investigated. The results showed that browning significantly decreased total phenolics and antioxidant capacity, with catechin, epicatechin, and B-type procyanidins being the most affected phenolics.
CURRENT RESEARCH IN FOOD SCIENCE
(2022)
Article
Crystallography
Kleanthi Sifaki, Nadiia Gumerova, Gerald Giester, Annette Rompel
Summary: The crystal structure of a polyoxometalate-based organic-inorganic hybrid compound is reported. The compound consists of a symmetrical hydroxyl-oxygen-octadeca-mu-oxido-octadecaoxidododecatungstate anion and is stabilized by sodium cations and water molecules, forming a three-dimensional framework structure.
ACTA CRYSTALLOGRAPHICA SECTION E-CRYSTALLOGRAPHIC COMMUNICATIONS
(2022)