Article
Chemistry, Multidisciplinary
Andrew J. J. Devine, Alice E. E. Parnell, Catherine R. R. Back, Nicholas R. R. Lees, Samuel T. T. Johns, Ainul Z. Z. Zulkepli, Rob Barringer, Katja Zorn, James E. M. Stach, Matthew P. P. Crump, Martin A. A. Hayes, Marc W. W. van der Kamp, Paul R. R. Race, Christine L. L. Willis
Summary: This study reveals the key epoxidation reaction in the synthesis of Abyssomicin C and the structure of the enzyme AbyV involved in this reaction. The combination of selective carbon-13 labeling with NMR spectroscopy proves to be an important tool in studying enzyme-catalyzed reactions in vitro.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Plant Sciences
Bianyun Yu, Nii Patterson, L. Irina Zaharia
Summary: Pulses, as important food crops, not only provide rich nutrients but also contain beneficial substances like saponins. However, there is still much to be explored regarding the biosynthesis of saponins in pulses. This review summarizes the research progress made in understanding saponin biosynthesis and its potential applications in improving the nutritional quality of pulses.
Article
Plant Sciences
Pramesti Istiandari, Shuhei Yasumoto, Hikaru Seki, Ery Odette Fukushima, Toshiya Muranaka
Summary: This study reveals that CPR classes I and II play different roles in triterpenoid biosynthesis in L. japonicus, with CPR class I potentially playing a crucial role in plant basal metabolism.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Lang Xiang, Jing Shi, Ao Zhu, Zi Fei Xu, Shuang He Liu, Yi Shuang Wang, Zhi Kai Guo, Rui Hua Jiao, Ren Xiang Tan, Hui Ming Ge
Summary: In this study, novel mutaxanthene analogues were discovered and the biosynthesis of these aromatic polyketides and their unusual xanthene framework was investigated. The complete biosynthetic pathway of mutaxathene was determined through gene deletion and biochemical experiments. A multifunctional FPMO, MtxO4, was found to catalyze ring rearrangement and generate the required xanthene ring. The total biosynthesis of mutaxanthene was successfully achieved in vitro. Our results reveal the formation of a rare xanthene ring in type II polyketide biosynthesis and demonstrate the potential of using total biosynthesis for the discovery of natural products synthesized by type II PKSs.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Peng Zhao, Hui Xue, Xiangzhen Zhu, Li Wang, Kaixin Zhang, Dongyang Li, Jichao Ji, Lin Niu, Xueke Gao, Junyu Luo, Jinjie Cui
Summary: The study identified and characterized a novel Spodoptera litura P450 gene, CYP321B1, which plays a crucial role in tannin detoxification and metabolism in insects. By conducting RNA interference and metabolome analysis, it was confirmed that silencing CYP321B1 significantly affects weight gain under tannin induction.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Chemistry, Organic
Karan Malhotra, Jakob Franke
Summary: This review provides a comprehensive overview of 149 functionally characterised CYP enzymes involved in the biosynthesis of triterpenoids and steroids in plants. It describes their phylogenetic distribution, reaction mechanisms, and highlights recent advancements in the field.
BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Review
Biotechnology & Applied Microbiology
Anup Adhikari, Sajan Shakya, Shreesti Shrestha, Dipa Aryal, Kavi Prasad Timalsina, Dipesh Dhakal, Yogan Khatri, Niranjan Parajuli
Summary: Cytochrome P450s are heme-binding monooxygenases that catalyze functionalization of various bonds, including heteroatom oxidation and C-C bond cleavage. Despite their biotechnological potential, P450s require redox partners for chemical transformations. This review focuses on the role of P450s in antibiotic biosynthesis, specifically macrolides, aminocoumarin antibiotics, NRPSs antibiotics, and RiPPs antibiotics.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
X. Xu, T. Hilberath, F. Hollmann
Summary: Heme-dependent oxygenases, such as P450 monooxygenases and peroxygenases, are selective catalysts for organic compound oxyfunctionalization. They exhibit mechanistic similarities and differences in CpdI formation, which may impact scalability, economic attractiveness, and environmental impact of their reactions. This contribution proposes performance indicators to compare the potential of both enzyme classes.
CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Peng Zhao, Hui Xue, Xiangzhen Zhu, Li Wang, Kaixin Zhang, Dongyang Li, Jichao Ji, Lin Niu, Xueke Gao, Junyu Luo, Jinjie Cui
Summary: In this study, a P450 gene CYP6AB12 was identified and characterized from Spodoptera litura, an insect species. The gene was found to play an important role in the detoxification and metabolism of gossypol, a plant allelochemical. Silencing the gene resulted in lowered expression levels and increased sensitivity to gossypol. These findings confirm the broad detoxification and metabolism abilities of P450s and provide a molecular basis for further research on detoxification metabolism and pest control.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Xueke Gao, Xiangzhen Zhu, Chuanpeng Wang, Li Wang, Kaixin Zhang, Dongyang Li, Jichao Ji, Lin Niu, Junyu Luo, Jinjie Cui
Summary: This study characterized AgoCYP6CY19, a CYP gene in Aphis gossypii, and found that its expression is related to developmental stages and host specialization. Silencing AgoCYP6CY19 increased mortality and reduced fecundity in aphids. Additionally, AgoCYP6CY19 was induced by certain plant substances. These findings highlight the importance of AgoCYP6CY19 in detoxification and metabolism processes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Aiko Teshima, Hisashi Kondo, Yu Tanaka, Yosi Nindita, Yuya Misaki, Yuji Konaka, Yasuhiro Itakura, Tsugumi Tonokawa, Haruyasu Kinashi, Kenji Arakawa
Summary: The study investigates the lankamycin biosynthetic pathway, focusing on two cytochrome P450 monooxygenases LkmK and LkmF. By constructing double mutants, it was confirmed that LkmK has a relaxed substrate specificity for 15-deoxy lankamycin derivatives, while LkmF has a strict substrate specificity in lankamycin biosynthesis, particularly recognizing the C-3 l-arcanosyl moiety.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Plant Sciences
Praveen Khatri, Owen Wally, Istvan Rajcan, Sangeeta Dhaubhadel
Summary: Cytochrome P450 monooxygenases (P450) play important roles in the catalytic conversion of biological compounds in plants. In this study, 346 P450 enzymes encoded by 317 genes were identified in soybean, and their composition, structure, expression, and distribution were compared. Analysis of transcriptomic datasets and co-expression analysis identified some P450 enzymes that may be involved in partial resistance against Phytophthora sojae. This study provides new insights into the functions of P450 enzymes in soybean metabolism.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jing Shen, Qian Yang, Lubo Hao, Lingling Zhang, Xuefeng Li, Mingqi Zheng
Summary: The newly discovered gene CYP77B34 was found to mediate Descurainia sophia L. resistance to tribenuron-methyl, and may have contributed to cross-resistance to other herbicides.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Anna Camus, Gisele Truong, Peer R. . E. Mittl, Greta Markert, Donald Hilvert
Summary: This study demonstrates the successful switch of substrate specificity in gatekeeper adenylation domains of nonribosomal peptide synthetases using high-throughput engineering, leading to efficient production of diverse peptide metabolites.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Qinghe Zhang, Fengbo Yang, Hong Tong, Yuan Hu, Xinyi Zhang, Tian Tian, Youjun Zhang, Qi Su
Summary: The ingestion of plant flavonoids enhances the tolerance of Bemisia tabaci to certain insecticides by increasing the activity of cytochrome P450 enzymes, which disrupts the mechanism of the insecticides.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Chemistry, Organic
Changli Sun, Zhijie Yang, Chunyan Zhang, Zhiyong Liu, Jianqiao He, Qing Liu, Tianyu Zhang, Jianhua Ju, Junying Ma
Article
Chemistry, Medicinal
Zhijie Yang, Xin Wei, Jianqiao He, Changli Sun, Jianhua Ju, Junying Ma
Review
Biotechnology & Applied Microbiology
Zhijie Yang, Jianqiao He, Xin Wei, Jianhua Ju, Junying Ma
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Article
Plant Sciences
Zhijie Yang, Changli Sun, Zhiyong Liu, Qing Liu, Tianyu Zhang, Jianhua Ju, Junying Ma
JOURNAL OF NATURAL PRODUCTS
(2020)
Article
Chemistry, Medicinal
Jianqiao He, Xin Wei, Zhijie Yang, Yan Li, Jianhua Ju, Junying Ma
Article
Plant Sciences
Ziwei Yao, Changli Sun, Yuhui Xia, Fang Wang, Liwu Fu, Junying Ma, Qinglian Li, Jianhua Ju
Summary: By precursor-directed biosynthesis, ten new actinomycin analogues were produced with impressive antimicrobial activities against clinically derived antibiotic resistant pathogens, including Staphylococcus aureus, Enterococcus faecium, and Candida albicans, while showing low cytotoxicity.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Biochemical Research Methods
Zhiying Fan, Nian Tong, Zhoukang Zhuang, Cheng Ma, Junying Ma, Jianhua Ju, Yanwen Duan, Xiangcheng Zhu
Summary: This research successfully increased the production of ilamycin-E1/E2 by optimizing the production process. Bacterial peptone was identified as a negative factor affecting the production, and it was replaced with malt extract to eliminate the accumulation of competitive byproducts, resulting in a 10.5-fold increase in titer. A pH coordinated feeding strategy further increased the production, but unsynchronization of feeding response and pH change hindered scaled-up production. Finally, a continuous pulse feeding strategy was used to solve this problem and achieved a 30.5-fold improvement in titer.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Jun Li, Zhiyong Liu, Mingye Hong, Changli Sun, Tianyu Zhang, Hua Zhang, Jianhua Ju, Junying Ma
Summary: Tuberculosis remains a global threat, especially with the emergence of multi-drug-resistant strains. Ilamycins, with potent anti-TB activities, show promise in treating TB with fast-onset effects.
FRONTIERS IN CHEMISTRY
(2021)
Article
Chemistry, Medicinal
Huaran Zhang, Yingying Chen, Yanqing Li, Yongxiang Song, Junying Ma, Jianhua Ju
Summary: The study on Streptomyces sp. SCSIO ZS0520 revealed new secondary metabolites and conducted bioinformatic analysis to lay foundations for solving remaining biosynthetic issues.
Article
Microbiology
Yingying Chen, Cunlei Cai, Jiafan Yang, Junjie Shi, Yongxiang Song, Dan Hu, Junying Ma, Jianhua Ju
Summary: An effective chemical transformation system and a gene disruption strategy based on CRISPR-Cas9 were successfully established for two marine-derived fungi, promoting the discovery of novel natural products and further biological studies.
Article
Plant Sciences
Cunlei Cai, Yingying Chen, Le Zhou, Naying Gong, Hua Zhang, Changli Sun, Junying Ma, Jianhua Ju
Summary: Three diphenyl ethers (1-3) and a cyclopentenone (4), along with seven known compounds (5-11), were isolated from the fermentation broth of the marine sediment-derived fungus Spiromastix sp. SCSIO F190. Compound 3 and 4 exist as atropisomers and racemates, respectively. These compounds showed activities against various pathogenic bacteria, with compounds 1-4 exhibiting strong activity against Gram-positive bacteria, including methicillin-resistant strains.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Chemistry, Organic
Huaran Zhang, Naying Gong, Hua Zhang, Qinglian Li, Junying Ma, Xiaoyi Wei, Wenli Li, Jianhua Ju
Summary: By using bioinformatics and biochemical methods, researchers confirmed that the methyltransferase and glycosyltransferase genes, mt3913 and gt723, respectively, are located beyond the boundaries of the actinopyrone biosynthetic gene cluster (atpn). In addition, the homologous protein GT1507 was able to produce 14 non-natural actinopyrone analogues. Among them, PM050463 (3) showed potent anti-Helicobacter pylori activity without any signs of cytotoxicity.
Article
Engineering, Marine
Ganesan Govindarajan, Ziwei Yao, Zhenbin Zhou, Xiaohong Zheng, Junying Ma, Pachaiyappan Saravana Kumar, Jianhua Ju, Changli Sun
Summary: In this study, the researchers sequenced the whole genome of Streptomyces griseus SCSIO PteL053, which was associated with the marine gastropod mollusk Planaxis sp. They discovered 28 putative biosynthetic gene clusters, including two responsible for the synthesis of therapeutic metabolites. Through detailed bioinformatics analysis, they identified the functions of the involved genes in the biosynthesis of known compounds. This complete genome sequencing allowed the rediscovery of clinically useful secondary metabolites in the newly isolated Streptomyces griseus.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2023)
Article
Microbiology
Yingying Chen, Jiafan Yang, Cunlei Cai, Junjie Shi, Yongxiang Song, Junying Ma, Jianhua Ju
Summary: Marine-derived fungi are valuable sources of structurally novel natural products with diverse bioactivities. Recyclable markers, such as pyrG and amdS, were successfully established and applied in two marine-derived fungi, Aspergillus sp. SCSIO SX7S7 and Spiromastix sp. SCSIO F190, respectively. These markers allowed the construction of markerless strains and facilitated genome mining for novel compounds.
Article
Biochemistry & Molecular Biology
Wenhui Zhou, Huan Fang, Qiuju Wu, Xinye Wang, Rong Liu, Fubin Li, Ji Xiao, Lin Yuan, Zhongmei Zhou, Junying Ma, Lulu Wang, Weiwei Zhao, Hua You, Jianhua Ju, Jing Feng, Ceshi Chen
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2019)
