期刊
CHEMBIOCHEM
卷 15, 期 7, 页码 950-954出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201400047
关键词
DNA strand displacement; DNAzymes; molecular logic; nucleic acids; sensors
资金
- National Science Foundation [1027877, 1028238]
- INCBN IGERT [DGE-0549500]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1026592, 1027877] Funding Source: National Science Foundation
- Directorate For Engineering
- Div Of Electrical, Commun & Cyber Sys [1028238] Funding Source: National Science Foundation
- Division of Computing and Communication Foundations
- Direct For Computer & Info Scie & Enginr [1318833] Funding Source: National Science Foundation
Chemical reactions catalyzed by DNAzymes offer a route to programmable modification of biomolecules for therapeutic purposes. To this end, we have developed a new type of catalytic DNA-based logic gates in which DNAzyme catalysis is controlled via toehold-mediated strand displacement reactions. We refer to these as DNAzyme displacement gates. The use of toeholds to guide input binding provides a favorable pathway for input recognition, and the innate catalytic activity of DNAzymes allows amplification of nanomolar input concentrations. We demonstrate detection of arbitrary input sequences by rational introduction of mismatched bases into inhibitor strands. Furthermore, we illustrate the applicability of DNAzyme displacement to compute logic functions involving multiple logic gates. This work will enable sophisticated logical control of a range of biochemical modifications, with applications in pathogen detection and autonomous theranostics.
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