期刊
CHEMBIOCHEM
卷 15, 期 13, 页码 1877-1881出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201402037
关键词
aptamers; DNA structures; emulsion PCR; molecular beacons; SELEX
资金
- National Institutes of Health [R21 CA157366, R21 CA182330]
We describe a new platform to identify structure-switching DNA beacon aptamers, which detect small molecules in a specific manner. By clonally amplifying a DNA library designed to fluoresce in response to binding events onto microbeads, aptamer beacons can be selected by stringent fluorescence-assisted sorting. We validated this method by isolating known and novel anti-steroid aptamers from two separate DNA libraries that were structurally enriched with three-way junctions. Importantly, aptamers were retrieved in only a few (three) rounds of selection by this approach and did not require further optimization, significantly streamlining the process of beacon development.
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