Protein Engineering of α/β-Hydrolase Fold Enzymes

The α/β-Hydrolase Fold 3DM Database (ABHDB) as a Tool for Protein Engineering

(2010) Robert Kourist et al.   CHEMBIOCHEM

Natural Diversity to Guide Focused Directed Evolution

(2010) Helge Jochens et al.   CHEMBIOCHEM

Rational Protein Design of Paenibacillus barcinonensis Esterase EstA for Kinetic Resolution of Tertiary Alcohols

(2010) Arnau Bassegoda et al.   ChemCatChem

Switching from an Esterase to a Hydroxynitrile Lyase Mechanism Requires Only Two Amino Acid Substitutions

(2010) Santosh Kumar Padhi et al.   CHEMISTRY & BIOLOGY

Protein engineering and discovery of lipases

(2010) Robert Kourist et al.   EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY

Thermostabilization of an esterase by alignment-guided focussed directed evolution

(2010) Helge Jochens et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture

(2010) C. K. Savile et al.   SCIENCE

Computational Design of an Enzyme Catalyst for a Stereoselective Bimolecular Diels-Alder Reaction

(2010) J. B. Siegel et al.   SCIENCE

Converting an Esterase into an Epoxide Hydrolase

(2009) Helge Jochens et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Increased Enantioselectivity by Engineering Bottleneck Mutants in an Esterase fromPseudomonas fluorescens

(2009) Anna Schließmann et al.   CHEMBIOCHEM

Finding better protein engineering strategies

(2009) Romas J Kazlauskas et al.   Nature Chemical Biology

Redesigning dehalogenase access tunnels as a strategy for degrading an anthropogenic substrate

(2009) Martina Pavlova et al.   Nature Chemical Biology

Directed evolution of an orthogonal nucleoside analog kinase via fluorescence-activated cell sorting

(2009) Lingfeng Liu et al.   NUCLEIC ACIDS RESEARCH

Single-Cell High-Throughput Screening To Identify Enantioselective Hydrolytic Enzymes

(2008) Stefan Becker et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Complete Inversion of Enantioselectivity towards Acetylated Tertiary Alcohols by a Double Mutant of aBacillus Subtilis Esterase

(2008) Sebastian Bartsch et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Novel Genetic Selection System for Improved Enantioselectivity ofBacillus subtilis Lipase A

(2008) Ykelien L. Boersma et al.   CHEMBIOCHEM

Addressing the Numbers Problem in Directed Evolution

(2008) Manfred T. Reetz et al.   CHEMBIOCHEM

Improved enantioselective conversion of styrene epoxides and meso-epoxides through epoxide hydrolases with a mutated nucleophile-flanking residue

(2008) Bert van Loo et al.   ENZYME AND MICROBIAL TECHNOLOGY

Kemp elimination catalysts by computational enzyme design

(2008) Daniela Röthlisberger et al.   NATURE

Highly active and selective endopeptidases with programmed substrate specificities

(2008) Navin Varadarajan et al.   Nature Chemical Biology

A one-pot, simple methodology for cassette randomisation and recombination for focused directed evolution

(2008) Aurelio Hidalgo et al.   PROTEIN ENGINEERING DESIGN & SELECTION