Article
Chemistry, Multidisciplinary
Daniela Melanie Delannoy Lopez, Dong Tien Tran, Guillaume Viault, Sofiane Dairi, Philippe Alexandre Peixoto, Yoan Capello, Laetitia Minder, Laurent Pouysegu, Elisabeth Genot, Carmelo Di Primo, Denis Deffieux, Stephane Quideau
Summary: The study chemically modified bioactive polyphenols for immobilization on sensor chips and used surface plasmon resonance to assess their interactions with various protein analytes, revealing differences in binding between different polyphenolic ligands and protein analytes. The multi-analysis SPR technique proved valuable for rapid screening and qualitative comparison of polyphenol-protein interactions.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Chemistry, Analytical
Benjamin Charron, Alexandre Delorme, Caroline Dubois, Maryam Hojjat Jodaylami, Jean-Francois Masson
Summary: This study reveals the significant impact of human serum albumin (HSA) on the response and binding constants of sensing assays, while bovine serum albumin (BSA) does not show the same effect. The results also highlight the importance of the matrix protein/analyte/receptor interactions in determining the assay response.
Article
Biochemistry & Molecular Biology
Alan Stampini Benhame de Castro, Hauster Maximiler Campos de Paula, Yara Luiza Coelho, Eliara Acipreste Hudson, Ana Clarissa S. Pires, Luis Henrique M. da Silva
Summary: Understanding nonionic surfactant-protein interactions is crucial for both technological and scientific purposes, yet there is a lack of kinetic data. By using surface plasmon resonance (SPR), this study investigated the kinetic and thermodynamic parameters of bovine lactoferrin-Brij58 interactions at different temperatures under physiological conditions. The results showed that the adsorption process was accelerated with increasing temperature, leading to a more thermodynamically stable complex formation. The energetic parameters indicated that the potential energy barrier for complex formation primarily arises from the reduction in system entropy.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Physics, Applied
Kishan Jayanand, Anupama B. Kaul
Summary: The study explores the plasmonic and excitonic interactions in two-dimensional (2D) semiconductors and zero-dimensional (0D) metal nanoparticles, revealing the tunability of the spectral response. The geometry of the hybrid structures affects the surface plasmon resonance (SPR) peaks, leading to a shift in the spectral response of the 2D semiconductor and influencing the carrier dynamics.
APPLIED PHYSICS LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Maciej Trzaskowski, Marcin Drozd, Tomasz Ciach
Summary: This study investigated the attachment process of saccharide particles to cell receptors responsible for glucose transport across the cell membrane. The interactions between simple saccharides, such as glucose and sucrose, and dextran nanoparticles with two species of GLUT proteins were measured. The results showed that polysaccharide nanoparticles interacted with GLUT proteins, and the strengths of interactions varied between proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Yuting Li, Dong He, Bing Li, Marianne N. Lund, Yifan Xing, Yi Wang, Fuxiang Li, Xiao Cao, Yujia Liu, Xiangyu Chen, Jiamei Yu, Jie Zhu, Minlian Zhang, Qiang Wang, Yuhao Zhang, Bin Li, Jinshui Wang, Xinhui Xing, Lin Li
Summary: Polyphenols, as plant secondary metabolites, have attracted more attention due to their potential health benefits. By engineering polyphenols through polyphenol-protein interaction, their stability, uptake, delivery, bioavailability, and biological activity can be improved. However, the safety of using polyphenol-protein complexes as functional food ingredients needs careful assessment.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Edwin J. Ortiz-Riano, Diana L. Mancera-Zapata, Martha Ulloa-Ramirez, Fernando Arce-Vega, Eden Morales-Narvaez
Summary: Macromolecular association is important in biomedical sciences. This study presents a real-time biosensing platform using graphene oxide-modified microwell plates and fluorophore-labeled proteins to determine protein-binding constants. The platform proves to be comparable and consistent with existing methods in determining protein kinetics constants.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Multidisciplinary
Ben Tadgell, Luis M. Liz-Marzan
Summary: Chiral plasmonic nanoparticles interact with biomolecules in distinct ways and can be probed using circular dichroism spectroscopy, offering potential advantages in biosensing and biomedical applications.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Analytical
Silvia Picciolini, Francesca Roda, Alice Gualerzi, Valentina Mangolini, Luana Forleo, Aurora Mangolini, Silvia Sesana, Antonia Antoniou, Francesca Re, Pierfausto Seneci, Marzia Bedoni
Summary: This study presents the use of Surface Plasmon Resonance imaging (SPRi) for characterization of multifunctionalized liposomes designed for targeted Glioblastoma treatment, showing promising results in targeting brain and diseased areas.
Article
Biochemistry & Molecular Biology
Agnieszka Paziewska-Nowak, Marcin Urbanowicz, Kamila Sadowska, Dorota Genowefa Pijanowska
Summary: The paper describes the development and utility of a novel DNA oligonucleotide-based affinity bioreceptor for lactoferrin. The research investigates the affinity of different types of oligonucleotides to the target protein using surface plasmon resonance method. The selected DNA sequence showed high affinity to lactoferrin and demonstrated its potential as a bioreceptor for lactoferrin determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Kheerthana Duraivelan, Sagarika Dash, Dibyendu Samanta
Summary: Nectins, a family of cell surface glycoproteins, mediate cell-cell adhesion and signaling pathways, with specific heterophilic interactions dictating their physiological relevance.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Food Science & Technology
Bianca Chima, Paul Mathews, Scott Morgan, Sarah A. Johnson, Charlene B. Van Buiten
Summary: Polyphenols are beneficial to human health, but their intake is low in the United States due to the low consumption of fruits and vegetables. Dairy foods such as milk and yogurt can increase the bioavailability of polyphenols through protein-polyphenol interactions. The study compared whey, pea, and hemp proteins for their ability to form complexes with polyphenols from blueberries. The results showed that pea and hemp proteins had stronger binding affinities with blueberry polyphenols than whey. The pH of the solution was found to play a role in protein-polyphenol complex formation.
Article
Chemistry, Medicinal
Peng He, Deling Shi, Yunran Li, Ke Xia, Seon Beom Kim, Rohini Dwivedi, Marwa Farrag, Vitor H. H. Pomin, Robert J. J. Linhardt, Jonathan S. S. Dordick, Fuming Zhang
Summary: Sulfated glycans from marine organisms have therapeutic activities and can inhibit the interactions between monkeypox virus proteins and heparin. This is important for developing broad-spectrum antiviral therapeutics for monkeypox.
Article
Chemistry, Multidisciplinary
Ziwen Jiang, Yu-Hsuan Kuo, Mengqi Zhong, Jianchao Zhang, Xin X. Zhou, Lijuan Xing, James A. Wells, Yanzhuang Wang, Michelle R. Arkin
Summary: Protein-protein interactions (PPIs) play crucial roles in cellular signaling and functions. Precise modulation of PPIs can help understand their roles in cellular events and be therapeutically valuable. In this study, an antibody fragment-based modulator for the PPI between p97 and its adaptor protein NSFL1C was developed, demonstrating its potential application in therapeutic interventions by disrupting specific intracellular PPIs.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Adrianne L. L. Gelbach, Fuming Zhang, Seok-Joon Kwon, John T. T. Bates, Andrew P. P. Farmer, Jonathan S. S. Dordick, Chunyu Wang, Robert J. J. Linhardt
Summary: Heparan sulfate (HS) acts as a co-receptor of angiotensin-converting enzyme 2 (ACE2) by interacting with the spike glycoprotein (SGP) of severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), facilitating the entry of the virus into host cells. This study analyzed the interactions between SARS-CoV-2 SGP receptor binding domain (RBD) and heparin using kinetic and structural analysis. The findings suggest that heparin plays a crucial role in the binding of SGP RBDs, with higher affinity observed for the Delta and Omicron variants compared to the wild type. The interactions between SGP RBDs and heparin are pH sensitive, with acidic pH enhancing binding and basic pH reducing binding, particularly for the Omicron variant.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Multidisciplinary Sciences
Johanne Mbianda, May Bakail, Christophe Andre, Gwenaelle Moal, Marie E. Perrin, Guillaume Pinna, Raphael Guerois, Francois Becher, Pierre Legrand, Seydou Traore, Celine Douat, Gilles Guichard, Francoise Ochsenbein
Summary: The study demonstrates that the structural plasticity of foldamer backbone is crucial for optimal interaction with protein surface. By designing a foldamer/peptide hybrid inhibitor using oligoureas as α-helix mimics, effective inhibition of the key regulator of chromatin dynamics, histone chaperone ASF1, was achieved. Additionally, the ASF1 ligands with nonpeptide oligourea segments showed significantly improved resistance to proteolysis in human plasma compared to alpha-helical peptides.
Article
Biochemistry & Molecular Biology
Lorene Tallet, Emilie Frisch, Megane Bornerie, Claire Medemblik, Benoit Frisch, Philippe Lavalle, Gilles Guichard, Celine Douat, Antoine Kichler
Summary: There is an urgent need to develop new therapeutic strategies to combat multidrug resistant bacteria. The use of urea-based foldamers has shown promising antibacterial and antifungal properties.
Article
Chemistry, Multidisciplinary
Franziska R. Traube, Marcel Stern, Annika J. Toelke, Martina Rudelius, Ernesto Mejias-Perez, Nada Raddaoui, Beate M. Kuemmerer, Celine Douat, Filipp Streshnev, Manuel Albanese, Paul R. Wratil, Yasmin Gaertner, Milda Nainyte, Grazia Giorgio, Stylianos Michalakis, Sabine Schneider, Hendrik Streeck, Markus Mueller, Oliver T. Keppler, Thomas Carell
Summary: In this study, chemically stabilized siRNA against SARS-CoV-2 was synthesized and modified with peptides, reducing viral loads and virus-induced cytotoxicity. The siRNA was also able to reduce virus replication and virus-induced apoptosis in 3D mucociliary lung microtissues. The adjustment of siRNA sequence allows rapid adaptation to different variants, and conjugation with receptor ligands via click-chemistry enables the construction of targeted siRNAs for a flexible antiviral defense strategy.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Sebastian Dengler, Celine Douat, Ivan Huc
Summary: In this study, hybrid sequences consisting of a peptide with multiple Cys residues and an aromatic foldamer helix with multiple chloroacetamide functions were synthesized. The reactive sites on the structurally defined helix were strategically placed to selectively stitch the peptide to form different macrocycles, resulting in mostly one preferred product. The reaction trails, involving identical reaction sites successively due to precise positioning in space, can be predicted based on structural considerations and the assumption that smaller macrocycles form faster.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Julien Henri, Laetitia Minder, Kevin Mohanasundaram, Sebastien Dilly, Anne Goupil-Lamy, Carmelo Di Primo, Anny Slama Schwok
Summary: This study identified new ligands of the nucleoprotein N of SARS-CoV-2 by in silico screening, using a refined Alphafold model of N and molecular dynamic simulations. The ligands, neuropeptides like substance P and enkephalin, impeded RNA binding to N and potentially altered the physiological roles of these neuropeptides. It was suggested that N may link viral replication and long COVID-19 symptoms, and targeted antivirals against N could improve patients' health.
Article
Chemistry, Multidisciplinary
Valentina Corvaglia, Florian Sanchez, Friedericke S. Menke, Celine Douat, Ivan Huc
Summary: Helically folded oligoamides composed of up to 41 units were efficiently prepared using optimized manual solid-phase synthesis (SPS) protocols. The high yield and purity of the products, as well as the validation of analytical methods including H-1 NMR, contribute to the efficiency of these protocols. Implementation of SPS on a commercial peptide synthesizer through in situ acid chloride activation under Appel's conditions dramatically reduces the labor required for producing long sequences, representing a breakthrough for the development of helical aromatic oligoamide foldamers.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Physics, Fluids & Plasmas
C. Douat, S. Ponduri, T. Boumans, O. Guaitella, S. Welzel, E. Carbone, R. Engeln
Summary: This study investigates the dissociation of CO2 in a symmetric pin-to-pin dielectric barrier discharge (DBD). The number of filaments per half cycle follows a power-law as a function of the injected power and remains constant regardless of pressure or other process parameters. The CO2 conversion is found to be proportional to specific energy input (SEI) even at the single filament level.
PLASMA SOURCES SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Sebastian Dengler, Ryan T. Howard, Vasily Morozov, Christos Tsiamantas, Wei-En Huang, Zhiwei Liu, Christopher Dobrzanski, Vojislava Pophristic, Sophie Brameyer, Celine Douat, Hiroaki Suga, Ivan Huc
Summary: Expanding the chemical diversity of peptide macrocycle libraries through the inclusion of a large aromatic helical foldamer has been implemented. A hybrid macrocyclic nanomolar binder to the C-lobe of the E6AP HECT domain was selected, and its crystal structure and molecular dynamics simulations revealed an intriguing reciprocal helical folding of the peptide and foldamer. The results represent a significant extension in the chemical space amenable to display selection and highlight the potential benefits of incorporating an aromatic foldamer into a peptide macrocycle for protein recognition.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biophysics
Daniel Dobrovodsky, Carmelo Di Primo
Summary: Surface plasmon resonance (SPR)-based biosensors are commonly used to study molecular interactions. The contribution of conformational changes to the SPR signal has been a subject of debate. This study demonstrates that conformational changes can indeed lead to a measured SPR signal.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Marie E. Perrin, Bo Li, Johanne Mbianda, May Bakail, Christophe Andre, Gwenaelle Moal, Pierre Legrand, Virginie Ropars, Celine Douat, Francoise Ochsenbein, Gilles Guichard
Summary: In this study, we investigated the substitution of 4 α-residues with 3-urea segments in a short α-helical peptide known to bind ASF1. By analyzing the effects of different foldamer replacements, we discovered new binding modes for the peptide-urea chimeras with ASF1.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Organic
Binhao Teng, Joan Atcher, Lars Allmendinger, Celine Douat, Yann Ferrand, Ivan Huc
Summary: Aromatic oligoamide foldamers were designed to fold into helical structures through local conformation preferences and solvophobic effects. Solid phase synthesis allowed for efficient synthesis of the desired sequences. Solvent-driven conformational transitions of the foldamers were observed using NMR and UV absorption spectroscopy, varying with sequence length.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Binhao Teng, Pradeep K. Mandal, Lars Allmendinger, Celine Douat, Yann Ferrand, Ivan Huc
Summary: This study designed and synthesized water-soluble helically folded aromatic oligoamides. By replacing charged side chains with neutral methoxy groups, various aggregates were formed and studied using different experimental methods. The results showed that the hydrophobic effect was the main driving force for aggregation and could be finely modulated by the presence or absence of charges.
Meeting Abstract
Biochemistry & Molecular Biology
Bo Li, Marie E. Perrin, Johanne Mbianda, May Bakail, Christophe Andre, Celine Douat, Raphael Guerois, Francoise Ochsenbein, Gilles Guichard
JOURNAL OF PEPTIDE SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Sebastian Dengler, Pradeep K. Mandal, Lars Allmendinger, Celine Douat, Ivan Huc
Summary: Incorporating an aromatic foldamer segment into a macrocyclic peptide can influence its conformation and properties. The study reveals that the foldamer helical folding tends to prevail and stretch peptides in a solvent-dependent manner, while peptides also reciprocally affect foldamer helix handedness bias and stabilization. The hybrid macrocycles exhibit resistance towards proteolytic degradation.
Article
Chemistry, Multidisciplinary
Megane Bornerie, Anais Brion, Gilles Guichard, Antoine Kichler, Celine Douat
Summary: Tailored helical CPFs with specific end-groups (pyridylthio- or n-octyl-ureas) have been reported as effective molecular systems in combination with helper lipids to intracellularly deliver biologically-relevant siRNA.
CHEMICAL COMMUNICATIONS
(2021)
