Article
Chemistry, Physical
Tomas Bohn Pessatti, Hernan Terenzi, Jean Borges Bertoldo
Summary: Chemical reactions can be used to covalently modify specific residues in proteins, expanding the building blocks for biocatalyst development. The most accessible targets are nucleophilic canonical amino acid sidechains, with a history of attempts to design enhanced or novel enzymes paving the way for a rapidly developing field.
Article
Chemistry, Organic
Nicholas D. J. Yates, Matthew E. Warnes, Reuben Breetveld, Christopher D. Spicer, Nathalie Signoret, Martin Fascione
Summary: alpha-Formylglycine (fGly) is a rare residue used as a precursor for pharmaceutically relevant motifs. However, the installation of fGly motifs into peptides is challenging due to degradation during solid-phase peptide synthesis. In this study, acid- and nucleophile-tolerant alpha-formylglycine building blocks were synthesized from vitamin C and successfully used to prepare callyaerin A, a macrocyclic peptide containing an fGly-derived motif.
Article
Chemistry, Multidisciplinary
Fan Ruxia, Johanna Hakanpaeae, Karoliina Elfving, Helena Taberman, Markus B. Linder, A. Sesilja Aranko
Summary: A type of protein/peptide pair called Catcher/Tag can spontaneously form an intermolecular isopeptide bond for biomolecular click reactions. Increasing the toolbox of orthogonal Catcher/Tag pairs is crucial to expand their applications, such as controlled multiple-fragment ligation. This study reports the engineering of novel Catcher/Tag pairs, including SilkCatcher/Tag, which enables pH-inducible protein ligation and is compatible with the widely used SpyCatcher/Tag pair. The SilkCatcher/Tag pair is also shown to be useful in producing native-sized highly repetitive spider-silk-like proteins with >90% purity, which is not achievable by traditional recombinant production methods.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Biotechnology & Applied Microbiology
Ruiqin Zhang, Chenghao Cao, Jiahua Bi, Yanjun Li
Summary: This article systematically summarizes the issues of protein engineering and post-translational modifications of fungal cellulases, pointing out the current challenges and opportunities, providing reference for further improvement of cellulase properties.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Martina Leso, Anna Kokla, Ming Feng, Charles W. Melnyk
Summary: The parasitic plant Phtheirospermum japonicum modifies the pectin methylesterification status in its haustoria in a dynamic and tissue-specific manner to allow efficient infection of the host plant.
Article
Chemistry, Multidisciplinary
Yu Zhou, Shuaidong Huo, Mark Loznik, Robert Goestl, Arnold J. Boersma, Andreas Herrmann
Summary: This study introduces a new method to control protein activity using ultrasound, by engineering protein structures to be sensitive to ultrasound and selectively activating or deactivating them. This concept could potentially serve as a blueprint for remotely controlling other bioactive molecules.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Rosa Merlo, Rosanna Mattossovich, Marianna Genta, Anna Valenti, Giovanni Di Mauro, Alberto Minassi, Riccardo Miggiano, Giuseppe Perugino
Summary: Self-labelling protein tags (SLPs) are versatile tools for sensor imaging that can be genetically fused with any protein of interest. Through computational and structural approaches, a thermostable CLIP-tag named SsOGT-MC8 was developed, which can covalently react with specific substrates at high temperatures.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Ross Thyer, Simon d'Oelsnitz, Molly S. Blevins, Dustin R. Klein, Jennifer S. Brodbelt, Andrew D. Ellington
Summary: The study developed new technology to site-specifically incorporate L-DOPA into recombinant proteins, providing a new approach for protein engineering applications.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Applied
Zuzana Meszaros, Lucie Petraskova, Natalia Kulik, Helena Pelantova, Pavla Bojarova, Vladimir Kren, Kristyna Slamova
Summary: Fungal beta-N-acetylhexosaminidases are important tools for the synthesis of natural and modified oligosaccharides and glycoconjugates. In this study, the enzyme from Aspergillus oryzae was engineered to shift its reaction from hydrolysis to transglycosylation. Nine mutant variants of the enzyme were designed and tested, and one variant (V306W/Y445N AoHex) showed superior transglycosidase activity with a transglycosylation-to-hydrolysis ratio greater than 110, which is unique among similar mutants.
ADVANCED SYNTHESIS & CATALYSIS
(2022)
Article
Chemistry, Physical
Bernd A. Nebel, Michael Breuer, Andreas Schneider, Benjamin Aberle, Stephan C. Hammer, Per-Olof Syren, Martin J. Weissenborn, Bettina M. Nestl
Summary: This article reflects on Professor Bernhard Hauer's distinguished career in biocatalysis and highlights his contributions to the application and development of enzymes.
Article
Biochemistry & Molecular Biology
Agne Savickaite, Gytis Druteika, Mikas Sadauskas, Vilius Malunavicius, Egle Lastauskiene, Renata Gudiukaite
Summary: The study analyzed the functionality of individual domains in fused lipolytic enzymes for the first time, showing that even if one domain is inactive, the other domain retains its activity, albeit with reduced specific activity. Experimental data suggested that the N-terminal domain mainly influenced thermostability, while the C-terminal domain was responsible for thermal activity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Biochemistry & Molecular Biology
Fabian B. H. Rehm, Tristan J. Tyler, Jing Xie, Kuok Yap, Thomas Durek, David J. Craik
Summary: Asparaginyl ligases are efficient transpeptidases capable of modifying proteins with a tripeptide recognition motif. Various protein modifications, such as N- and C-terminal labeling, head-to-tail cyclization, and protein-protein conjugation, can be achieved using these enzymes. Efforts to engineer highly efficient ligases and manipulate enzyme reactions chemically have expanded the capabilities of protein engineers.
Article
Chemistry, Multidisciplinary
Joel H. Z. Lee, Matthew N. Podgorski, Michael Moir, Alecia R. Gee, Stephen G. Bell
Summary: Cytochrome P450 monooxygenase enzymes can be converted into peroxygenases by mutating a conserved active site threonine to a glutamate, resulting in higher stability and reactivity towards hydrogen peroxide. These peroxygenase variants offer the possibility of simpler cytochrome P450 systems for selective oxidations.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Daniel Salas-Lloret, Roman Gonzalez-Prieto
Summary: Ubiquitination and SUMOylation are dynamic post-translational modifications that regulate cellular processes. The understanding of these modifications has been increased by the development of mass spectrometry-based approaches. This translated article provides a concise overview of the basic mechanisms of ubiquitination and SUMOylation, as well as recent MS-based approaches for specific target identification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Food Science & Technology
Anshula Sharma, Gaganjot Gupta, Tawseef Ahmad, Sheikh Mansoor, Baljinder Kaur
Summary: Enzyme engineering techniques, such as directed evolution and site-directed mutagenesis, play a crucial role in enhancing catalytic properties, improving industrial efficiency, and producing novel metabolites. These strategies offer excellent opportunities to tailor existing enzymes and construct highly efficient industrial enzymes for cost-effective production of value-added products.
FOOD REVIEWS INTERNATIONAL
(2021)
Article
Biophysics
Alexander Harder, Ann-Kristin Moeller, Fabian Milz, Phillipp Neuhaus, Volker Walhorn, Thomas Dierks, Dario Anselmetti
BIOPHYSICAL JOURNAL
(2015)
Editorial Material
Biochemistry & Molecular Biology
Jianhe Peng, Sarfaraz Alam, Karthikeyan Radhakrishnan, Malaiyalam Mariappan, Markus Georg Rudolph, Caroline May, Thomas Dierks, Kurt von Figura, Bernhard Schmidt
Article
Oncology
Susmita Mondal, Debarshi Roy, Juliana Camacho-Pereira, Ashwani Khurana, Eduardo Chini, Lifeng Yang, Joelle Baddour, Katherine Stilles, Seth Padmabandu, Sam Leung, Steve Kalloger, Blake Gilks, Val Lowe, Thomas Dierks, Edward Hammond, Keith Dredge, Deepak Nagrath, Viji Shridhar
Article
Cell Biology
Heike Wolf, Markus Damme, Stijn Stroobants, Rudi D'Hooge, Hans Christian Beck, Irm Hermans-Borgmeyer, Renate Luellmann-Rauch, Thomas Dierks, Torben Luebke
DISEASE MODELS & MECHANISMS
(2016)
Article
Endocrinology & Metabolism
Ilona Jaszczuk, Lars Schlotawa, Thomas Dierks, Andreas Ohlenbusch, Dominique Koppenhoefer, Mariusz Babicz, Monika Lejman, Karthikeyan Radhakrishnan, Agnieszka Lugowska
MOLECULAR GENETICS AND METABOLISM
(2017)
Article
Cell Biology
J. H. Kim, C. Chan, C. Elwell, M. S. Singer, T. Dierks, H. Lemjabbar-Alaoui, S. D. Rosen, J. N. Engel
CELLULAR MICROBIOLOGY
(2013)
Article
Biochemistry & Molecular Biology
Bjoern Kowalewski, Peter Heimann, Theresa Ortkras, Renate Luellmann-Rauch, Tomo Sawada, Steven U. Walkley, Thomas Dierks, Markus Damme
HUMAN MOLECULAR GENETICS
(2015)
Article
Biochemistry & Molecular Biology
Bjoern Kowalewski, Torben Luebke, Katrin Kollmann, Thomas Braulke, Thomas Reinheckel, Thomas Dierks, Markus Damme
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Juliane Poppe, Eberhard Warkentin, Ulrike Demmer, Bjoern Kowalewski, Thomas Dierks, Klaus Schneider, Ulrich Ermler
JOURNAL OF INORGANIC BIOCHEMISTRY
(2014)
Article
Multidisciplinary Sciences
Myungsik Yoo, Muntasir Khaled, Kurt M. Gibbs, Jonghun Kim, Bjoern Kowalewski, Thomas Dierks, Melitta Schachner
Article
Multidisciplinary Sciences
Inna Maltseva, Matilda Chan, Ina Kalus, Thomas Dierks, Steven D. Rosen
Article
Multidisciplinary Sciences
Ina Kalus, Susanne Rohn, Tania M. Puvirajesinghe, Scott E. Guimond, Pieter J. Eyckerman-Koelln, Gerdy ten Dam, Toin H. van Kuppevelt, Jeremy E. Turnbull, Thomas Dierks
Article
Multidisciplinary Sciences
Debarshi Roy, Susmita Mondal, Ashwani Khurana, Deok-Beom Jung, Robert Hoffmann, Xiaoping He, Eleftheria Kalogera, Thomas Dierks, Edward Hammond, Keith Dredge, Viji Shridhar
SCIENTIFIC REPORTS
(2017)
Article
Biochemistry & Molecular Biology
Omkar P. Dhamale, Roger Lawrence, Elena M. Wiegmann, Bhahwal A. Shah, Kanar Al-Mafraji, William C. Lamanna, Torben Luebke, Thomas Dierks, Geert-Jan Boons, Jeffrey D. Esko
ACS CHEMICAL BIOLOGY
(2017)
Article
Ophthalmology
Katharina Kruszewski, Renate Luellmann-Rauch, Thomas Dierks, Udo Bartsch, Markus Damme
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2016)
