期刊
CEREBRAL CORTEX
卷 19, 期 3, 页码 697-702出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/cercor/bhn118
关键词
compartmentalization; FLIP; lateral diffusion; photobleaching; plasticity
资金
- Swiss National Science Foundation [31-61518.00]
- Canadian Institutes of Health Research
- Canadian Foundation of Innovation
- National Sciences and Engineering Research Council of Canada
- MRC [MC_U120088464] Funding Source: UKRI
- Medical Research Council [MC_U120088464] Funding Source: researchfish
The purpose of this study was to examine whether variability in the shape of dendritic spines affects protein movement within the plasma membrane. Using a combination of confocal microscopy and the fluorescence loss in photobleaching technique in living hippocampal CA1 pyramidal neurons expressing membrane-linked GFP, we observed a clear correlation between spine shape parameters and the diffusion and compartmentalization of membrane-associated proteins. The kinetics of membrane-linked GFP exchange between the dendritic shaft and the spine head compartment were slower in dendritic spines with long necks and/or large heads than in those with short necks and/or small heads. Furthermore, when the spine area was reduced by eliciting epileptiform activity, the kinetics of protein exchange between the spine compartments exhibited a concomitant decrease. As synaptic plasticity is considered to involve the dynamic flux by lateral diffusion of membrane-bound proteins into and out of the synapse, our data suggest that spine shape represents an important parameter in the susceptibility of synapses to undergo plastic change.
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