Article
Biochemistry & Molecular Biology
Mayya P. Razgonova, Alexander M. Zakharenko, Elena Gordeeva, Olesya Yu Shoeva, Elena Antonova, Konstantin S. Pikula, Liudmila A. Koval, Elena K. Khlestkina, Kirill S. Golokhvast
Summary: This study conducted a metabolomic analysis of grains from seven genetically related wheat lines, revealing the presence of various biologically active polyphenolic compounds. Despite the genetic similarity between the lines, specific individual compounds were identified that could be used as unique chemical fingerprints to distinguish each line. The influence of genotype on the chemical profiles of wheat grains was also discussed.
Article
Agronomy
Mariangela Marrelli, Pasqualina Sprovieri, Filomena Conforti, Giancarlo Statti
Summary: The locally grown ancient wheat varieties Carosella and Majorca showed high polyphenol content and antioxidant activity. Whole wheat flour exhibited the highest antioxidant activity, indicating that these two wheat varieties could be potential sources of antioxidants with significant health benefits.
Article
Agronomy
Elena Gordeeva, Olesya Shoeva, Sergey Mursalimov, Irina Adonina, Elena Khlestkina
Summary: This study successfully created black-grained substitution lines in the genetic background of bread wheat using marker-assisted technology, allowing for the simultaneous accumulation of anthocyanins in the aleurone and pericarp layers with a high total anthocyanin content. Although chromosome substitution had a positive effect on the main spike length, it negatively affected other yield-related traits.
Article
Plant Sciences
Stefan M. Markovic, Dragan Zivancev, Daniela Horvat, Aleksandra Torbica, Jovana Jovankic, Nevena H. Djukic
Summary: Heat stress is a significant factor affecting wheat production, with eEF1A playing a role in plant heat tolerance. Varieties with higher eEF1A accumulation under heat stress show less decrease in photosynthetic pigments, correlating with better yield traits. This study aims to develop agricultural strategies and heat-tolerant wheat varieties through analyzing molecular mechanisms under high and moderate air temperatures.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Plant Sciences
Qinqin Jiang, Wenhui Jiang, Ning Hu, Rui Tang, Yuxuan Dong, Hongqi Wu, Tianxiang Liu, Lulu Guan, Hanbing Zhang, Junbin Hou, Guaiqiang Chai, Zhonghua Wang
Summary: Anthocyanin biosynthesis in the pericarps of purple-grained wheat is light-dependent, but the regulatory mechanisms are unknown. Two key genes, TaHY5-7A and TaBBX-3B, were identified and found to collaborate in activating the expression of TaPpm1, promoting light-induced anthocyanin biosynthesis in purple-pericarp wheat.
Article
Agronomy
Ruiqi Zhang, Chuntian Lu, Xiangru Meng, Yali Fan, Jie Du, Runran Liu, Yigao Feng, Liping Xing, Petr Capal, Jaroslav Dolezel, Yiwei Wang, Huanqing Mu, Bingxiao Sun, Fu Hou, Ruonan Yao, Chuanxi Xiong, Yang Wang, Peidu Chen, Aizhong Cao
Summary: This study introgressed the novel wheat powdery mildew and stripe rust resistance genes Pm5V/Yr5V from Dasypyrum villosum and fine mapped them to a narrowed region in 5VS. The results showed that the Pm5V/Yr5V lines have the potential value to facilitate wheat breeding for disease resistance and do not incur a yield penalty in diverse wheat genetic backgrounds.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Plant Sciences
Guangbin Luo, Lisha Shen, Shancen Zhao, Ruidong Li, Yanhong Song, Shuyi Song, Kang Yu, Wenlong Yang, Xin Li, Jiazhu Sun, Yanpeng Wang, Caixia Gao, Dongcheng Liu, Aimin Zhang
Summary: The study identified 71 transcription factors coexpressed with SSP genes in the endosperm of T. urartu, with TuNAC77 and TaNAC77 enhancing SSP gene transcription by binding to cis-elements in promoters. Knocking down TaNAC77 in common wheat resulted in a 24% reduction in total SSPs. This is the first genome-wide identification of transcriptional regulators of SSP genes in wheat.
Article
Agronomy
Linyuan Duan, Bo Zhang, Guoli Dai, Xinru He, Xuan Zhou, Ting Huang, Xiaojie Liang, Jianhua Zhao, Ken Qin
Summary: Wolfberry is a small shrub with medicinal and edible properties, mainly available in red, purple, and yellow phenotypes. It is rich in flavonoids, which contribute to the diverse colors of plants. This study analyzed the metabolite and gene expression profiles of different colored wolfberries and identified the molecular mechanism underlying their color differences. The accumulation of bluish anthocyanins was found in purple wolfberries, while red and yellow wolfberries accumulated more yellowish flavonoids. Additionally, differentially expressed genes related to flavonoid synthesis and transcription factors were identified among wolfberries of different colors. These findings provide new insights into the molecular mechanism of fruit color differences in wolfberry and offer new ideas for molecular breeding.
Article
Forestry
Ling Su, Meng Qi, Dong Meng, Qing Yang, Yongmei Wang, Fengshan Ren, Liying Yang, Yingchun Chen, Liyuan Liu, Meiling Tang, Yangbo Song, Lei Gong
Summary: In order to cultivate different grape varieties according to market needs, it is important to study the regulation mechanism of color changes in grapes at different development stages. This study used RNA-sequencing technology to compare and analyze the transcriptome data of four grape varieties at the same development stage. The anthocyanin synthesis pathway in the flavonoid pathway was mainly studied among the annotated differential genes. The role of the MYB transcription factor MYB2 in anthocyanin regulation was confirmed through subcellular localization and gene function verification.
Article
Plant Sciences
Suwen Lu, Yaxian Zhuge, Tianyi Hao, Zhongjie Liu, Mengwei Zhang, Jinggui Fang
Summary: In this study, 47 OMT members were identified in the grape genome and their characteristics, distribution, expression, and regulation were investigated. The results showed that some OMT members are involved in the synthesis of methylated flavonoids and play important roles in the growth and defense of grapes.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Food Science & Technology
Serafino Suriano, Pasquale Codianni, Anna Iannucci
Summary: This study investigated the content of carotenoids and tocols in different genotypes of wheat species and found that Triticum durum had the highest carotenoid content, while Triticum dicoccum had a lower tocols content compared to other species. There were positive correlations between tocols and 13/gamma tocotrienols, and different genotypes from the same species were grouped into different clusters.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Plant Sciences
Renxiang Lu, Miaoyu Song, Zhe Wang, Yanlei Zhai, Chaoyang Hu, Avihai Perl, Huiqin Ma
Summary: Red flesh is a desired trait in grapes, but its regulation is not well understood. In this study, we analyzed the flavonoid metabolomes and transcriptomes of the flesh and skin of the grape variety 'Mio Red'. We found significant differences in flavonoid levels between the flesh and skin, and identified differentially expressed genes involved in flavonoid metabolism.
Article
Chemistry, Multidisciplinary
Md Ahsan Habib, Shuwang Wu, Qingrui Fan, Thomas Odey Magu, Xi Yao, Jianyong Lv, Jianjun Wang
Summary: Inspired by the biological self-recovery mechanism of superhydrophobicity, a new class of waxgel material has been reported, which features sustainable hierarchical surface micro-structures and the ability to self-reconstruct its surface layer after damage or removal. This material does not require external input for self-recovery and exhibits durability and non-fluorinated composition.
CHEMICAL COMMUNICATIONS
(2021)
Article
Food Science & Technology
Hui Xia, Xuefeng Zhang, Yanqiu Shen, Yuqi Guo, Tong Wang, Jin Wang, Lijin Lin, Honghong Deng, Qunxian Deng, Kunfu Xu, Xiulan Lv, Dong Liang
Summary: In this study, the flavonoid metabolites in red 'Summer Black' and white 'Shine Muscat' table grapes were compared. The results showed that, during fruit development, 'Summer Black' had higher flavonoid compositions and content, especially anthocyanins. On the other hand, 'Shine Muscat' had higher content of flavonols. The expression levels of anthocyanin-related genes were higher in 'Summer Black', while 'Shine Muscat' showed higher expression levels of F3H and FLS genes.
JOURNAL OF FOOD SCIENCE
(2022)
Article
Plant Sciences
Liangjie Lv, Ce Dong, Yuping Liu, Aiju Zhao, Yelun Zhang, Hui Li, Xiyong Chen
Summary: This study identified differentially expressed genes and differential accumulated metabolites in wheat under cold stress. The comprehensive analysis of metabolomics and transcriptome revealed that the cold resistance of wheat is closely related to several metabolites and key enzymes in specific biosynthesis pathways. Additionally, several candidate genes were found to be involved in the cold resistance of wheat.
Review
Biochemistry & Molecular Biology
Sabina Lipnicanova, Daniela Chmelova, Miroslav Ondrejovic, Vladimir Frecer, Stanislav Miertus
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Review
Chemistry, Analytical
Barbora Legerska, Daniela Chmelova, Miroslav Ondrejovic, Stanislav Miertus
Summary: Microorganisms and plants are important sources of compounds with potential pharmaceutical applications. TLC-Bioautography combines separation and biological analysis to screen extracts for enzyme inhibitors. This review summarizes the methods and applications of TLC-Bioautography and discusses its potential future developments.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
V. Peckova, B. Legerska, D. Chmelova, M. Hornik, M. Ondrejovic
Summary: This study found that white-rot fungi can be used as a suitable and low-cost biosorbent for the removal of azo dyes from water, with the predominant mechanism being biosorption.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Sabina Lipnicanova, Daniela Chmelova, Andrej Godany, Miroslav Ondrejovic, Stanislav Miertus
JOURNAL OF BIOTECHNOLOGY
(2020)
Review
Biotechnology & Applied Microbiology
Zuzana Hlasova, Lucia Pazitna, Miroslav Ondrejovic, Jaroslav Katrlik
Summary: Small molecule inhibitors of neuraminidases are promising for treating influenza viruses. In vitro determination of their inhibition activity is important, but traditional methods may have interference issues. A lectin-based assay showed good sensitivity and avoided toxic reagents, offering benefits for NA inhibitor testing.
JOURNAL OF BIOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Daniela Chmelova, Barbora Legerska, Jana Kunstova, Miroslav Ondrejovic, Stanislav Miertus
Summary: This review provides an overview of laccase production by white-rot fungi under solid-state fermentation conditions. The variations in the up-stream, fermentation, and down-stream processes and their impact on laccase activity are discussed. The studies suggest that a suitable combination of lignocellulosic material and laccase producer can shorten the production process, and the utilization of waste materials can reduce costs and increase competitiveness.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sabina Lipnicanova, Barbora Legerska, Daniela Chmelova, Miroslav Ondrejovic, Stanislav Miertus
Summary: This study successfully expressed enzymatically active NA in Escherichia coli, providing a valuable foundation for the development of new antiviral agents against influenza.
Article
Biotechnology & Applied Microbiology
Sona Rond'osova, Barbora Legerska, Daniela Chmelova, Miroslav Ondrejovic, Stanislav Miertus
Summary: This study optimized the composition of the minimal propagation medium for Cupriavidus necator DSM 545 using response surface methodology (RSM), and evaluated the optimal composition of the production medium. The results showed that with appropriate conditions set, high biomass production and PHA yield can be achieved.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Daniela Chmelova, Barbora Legerska, Miroslav Ondrejovic, Stanislav Miertus
Summary: The study aimed to optimize a synthetic medium for maximum biomass yield using RSM, achieving a biomass yield of 1.71 g/L due to the optimal carbon and nitrogen sources in the medium. This approach demonstrates the potential of using two-stage PHA cultivation to obtain the maximum amount of biomass and PHA.
FERMENTATION-BASEL
(2022)
