期刊
JOURNAL OF FOOD AND DRUG ANALYSIS
卷 23, 期 4, 页码 766-777出版社
FOOD & DRUG ADMINSTRATION
DOI: 10.1016/j.jfda.2015.07.002
关键词
antioxidant; colon cancer cells; extraction; fucoidan; Sargassum cristaefolium
资金
- National Science Council of the Republic of China [NSC 101-2321-B-022-001-MY2]
- Kaohsiung Medical University Hospital, Kaohsiung Medical University
Fucose-containing sulfated polysaccharides, also termed fucoidans, which are known to possess antioxidant, anticoagulant, anticancer, antiviral, and immunomodulating properties, are normally isolated from brown algae via various extraction techniques. In the present study, two methods (SC1 and SC2) for isolation of fucoidan from Sargassum cristaefolium were compared, with regard to the extraction yields, antioxidant activity, and inhibition of growth of human colon cancer cells exhibited by the respective extracts. SC1 and SC2 differ in the number of extraction steps and concentration of ethanol used, as well as the obtained sulfated polysaccharide extracts, namely, crude fucoidan preparation (CFP) and purified fucoidan preparation (PFP), respectively. Thin layer chromatography, Fourier transform infrared analysis, and measurements of fucose and sulfate contents revealed that the extracts were fucoidan. There was a higher extraction yield for CFP, which contained less fucose and sulfate but more uronic acid, and had weaker antioxidant activity and inhibition of growth in human colon cancer cells. In contrast, there was a lower extraction yield for PFP, which contained more fucose and sulfate but less uronic acid, and had stronger antioxidant activity and inhibition of growth in human colon cancer cells. Thus, since the difference in bioactive activities between CFP and PFP was not remarkable, the high extraction yield of SC1 might be favored as a method in industrial usage for extracting fucoidan. Copyright (C) 2015, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC. All rights reserved.
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