Article
Biochemistry & Molecular Biology
Hiroaki Kaku, Allison R. Balaj, Thomas L. Rothstein
Summary: This study found that Fas Apoptosis Inhibitory Molecule (FAIM) can prevent protein aggregation during cellular stress, especially the aggregation of amyloid-beta protein, a pathological hallmark of Alzheimer's disease. The study also revealed the role of FAIM in preventing protein aggregation in conjunction with small heat shock proteins (sHSPs). These findings provide new insights into the interrelationships among FAIM, sHSPs, and amyloid-beta aggregation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Zhanwu Yang, Hui Du, Xinzhu Xing, Wenlong Li, Youbin Kong, Xihuan Li, Caiying Zhang
Summary: This study identified an important sHSP protein GmHSP17.9 in soybean nodules, revealing its significant role in nodule development and nitrogen fixation. GmHSP17.9 interacts with GmNOD100, influencing the function of soybean nodules.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Plant Sciences
Zhanwu Yang, Hui Du, Jingyi Sun, Xinzhu Xing, Youbin Kong, Wenlong Li, Xihuan Li, Caiying Zhang
Summary: GmHSP17.1 plays an important role in soybean nodulation by interacting with GmRIP1, regulating nodule development, nitrogen fixation activity, peroxidase activity, and reactive oxygen species levels. These findings are crucial for studying the mechanism of nitrogen fixation and genetic improvement of legume plants.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Artur Pirog, Francesca Cantini, Lukasz Nierzwicki, Igor Obuchowski, Bartlomiej Tomiczek, Jacek Czub, Krzysztof Liberek
Summary: Small heat shock proteins are a class of ATP-independent chaperones that prevent protein aggregation in stress conditions and facilitate refolding by ATP-dependent chaperones. In some Enterobacterales, a two-protein sHsp system has evolved from a gene duplication event, where the two proteins interact, influence each other's expression and degradation. The formation of a heterodimer between IbpA and IbpB is found to be the functional unit of this system, similar to the homodimers of single bacterial sHsps.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Plant Sciences
Tung-Yi Lin, Wei-Jyun Hua, Hsin Yeh, Ai-Jung Tseng
Summary: The study demonstrates that FIPs LZ-8 and GMI induce changes in the proteomic profile of tumor lesions in LLC1 cell-bearing mouse, potentially regulating heat shock proteins (HSPs)-related cell viability and inhibiting cell migration and inducing apoptosis. Inhibition of HSPs may contribute to the anti-lung cancer activity of FIPs.
Article
Cell Biology
Vinicius M. Gomes, Rosangela A. M. Wailemann, Gabriel S. Arini, Talita C. Oliveira, Daria R. Q. Almeida, Ancely F. dos Santos, Leticia F. Terra, Stephan Lortz, Leticia Labriola
Summary: During the development of type 1 diabetes mellitus (T1DM), islet transplantation is considered an attractive alternative for beta-cell replacement therapy. Increasing levels of heat-shock protein B1 (HSPB1) can enhance beta-cell resistance to proinflammatory cytokines and oxidative stress-induced apoptosis, potentially contributing to the optimization of beta-cell replacement therapies.
Review
Biochemistry & Molecular Biology
Igor Obuchowski, Piotr Karas, Krzysztof Liberek
Summary: Small heat shock proteins (sHsps) are a class of ATP-independent chaperones that play a crucial role during protein stress, forming assemblies with aggregation-prone protein substrates to prevent irreversible aggregation. These assemblies facilitate subsequent chaperone-dependent disaggregation and refolding of substrates.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Cell Biology
Tong Lei, Zhuangzhuang Xiao, Wangyu Bi, Shanglin Cai, Yanjie Yang, Hongwu Du
Summary: Neurodegenerative diseases are aging-related diseases involving neuron death. Small heat shock proteins play a crucial role in maintaining cellular homeostasis and resisting toxic protein aggregation. Research suggests that enhancing HSPB expression and delivery can improve manifestations of neurodegenerative diseases.
AGEING RESEARCH REVIEWS
(2022)
Article
Physiology
Jiayue Zhou, Jing Wu, Sifan Zheng, Xiangjun Chen, Daizhan Zhou, Xingchao Shentu
Summary: Cold cataract refers to the reversible opacification of the lens as temperature decreases, but prolonged exposure to low temperature can lead to irreversible opacification. Transcriptomic analysis showed up-regulation of genes related to lens structure and development, with small heat shock proteins identified as hub genes. Proteomic analysis revealed up-regulation of the apoptotic process, and integrated analysis suggested down-regulation of specific signaling pathways as part of the regulatory mechanism in the irreversible opacification process.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Food Science & Technology
Kaifang Guan, Xiaofen Qi, Haoran Chen, Ying Ma
Summary: Mitochondria-dependent myoblast apoptosis induced by oxidative stress and decrease in protein synthesis plays a crucial role in sarcopenia. Milk fat globule-EGF factor VIII (MFG-E8) can protect against mitochondrial injury and apoptosis through regulation of mitochondrial function and apoptosis-related factors. The findings provide a promising approach for mitigating sarcopenia.
Article
Biochemical Research Methods
Aikaterini G. Karra, Aikaterini Sioutopoulou, Vyron Gorgogietas, Martina Samiotaki, George Panayotou, Anna-Maria G. Psarra
Summary: This study characterized mitochondrial glucocorticoid receptor interacting proteins, revealing potentially new regulatory roles of the receptor in mitochondria. The interactions between mtGR and PDH, as well as mtGR and HSP60, suggest involvement of mtGR in energy production regulation and mitochondrial import processes. These findings uncover potential novel regulatory roles of mtGR in coordinating nuclear and mitochondrial functions.
JOURNAL OF PROTEOMICS
(2022)
Article
Virology
Savita Chaudhary, Vijayanandraj Selvaraj, Preshika Awasthi, Swati Bhuria, Rituraj Purohit, Surender Kumar, Vipin Hallan
Summary: The small heat shock protein 22.98 (sHsp22.98) from Trialeurodes vaporariorum binds to ASSVd RNA and plays a significant role in its transmission.
Article
Biochemistry & Molecular Biology
V. Sudhakar Reddy, S. Pandarinath, M. Archana, G. Bhanuprakash Reddy
Summary: Chronic hyperglycemia induces differential responses of sHsps by altering their expression, solubility, interaction, and roles in apoptosis in neuronal cells, potentially contributing to cell death.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Nanoscience & Nanotechnology
Zhenxing Wang, Cuimei Liu, Yingjiu Zhang, Chungang Wang
Summary: This study proposes an energy depletion-mediated heat-sensitization strategy to reduce cellular thermal resistance and enhance tumor-specific photothermal therapy. A novel nano-material was synthesized for drug loading, enabling controlled release and enhancing heat sensitivity by inhibiting tumor glycolysis and mitochondrial dysfunction, thereby improving therapeutic efficiency.
ACS APPLIED NANO MATERIALS
(2023)
Article
Environmental Sciences
Milton Talukder, Shao-Shuai Bi, Mei-Wei Lv, Jing Ge, Cong Zhang, Jin-Long Li
Summary: Cadmium exposure induces neuronal damage in the chicken brain, triggering the heat shock response (HSR) pathway and apoptotic pathway in response to the damage.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Cell Biology
Ke Mi, Lizhong Zeng, Yang Chen, Jingya Ning, Siyuan Zhang, Peilin Zhao, Shuanying Yang
Summary: In this study, the researchers explored the role of DHX38 in NSCLC and its underlying molecular mechanism. They found that DHX38 was overexpressed in NSCLC and patients with high DHX38 expression had poor prognosis. DHX38 promoted cell proliferation, migration, and invasion in NSCLC and activated the MAPK pathway. The researchers also identified G3BP1 as a target protein that interacted with DHX38 and showed that DHX38 regulated the expression of G3BP1. Silencing G3BP1 reversed the effects of DHX38 overexpression on tumor cell proliferation, migration, and invasion and inhibited the MAPK pathway activation.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Tiina A. Jokela, Mark A. Dane, Rebecca L. Smith, Kaylyn L. Devlin, Sundus Shalabi, Jennifer C. Lopez, Masaru Miyano, Martha R. Stampfer, James E. Korkola, Joe W. Gray, Laura M. Heiser, Mark A. Labarge
Summary: Microenvironment signals have a significant impact on cell fate and tissue homeostasis. Understanding how different microenvironment factors regulate cellular phenotype has been challenging. In this study, a high-throughput microenvironment microarray was used to identify factors that support the proliferation and maintenance of primary human mammary luminal epithelial cells. Multiple factors that modulate luminal cell number were identified and their effects were confirmed using RNA sequencing and cell-based functional studies. Hepatocyte growth factor (HGF) was found to be robust to individual variation and played a role in expanding luminal cells. Our approach demonstrates the power of high-dimensional cell-based approaches in dissecting microenvironmental signals.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chao He, Yongfeng Ding, Yan Yang, Gang Che, Fei Teng, Haohao Wang, Jing Zhang, Donghui Zhou, Yanyan Chen, Zhan Zhou, Haiyong Wang, Lisong Teng
Summary: This study categorized gastric cancer patients into three stemness subtypes, each demonstrating distinct prognoses, components of tumor microenvironment (TME) infiltration, and varying sensitivity or resistance to treatment. A stemness risk model was constructed to predict treatment response and prognosis.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Haile Zhao, Lijuan Feng, Rui Cheng, Man Wu, Xiaozhou Bai, Lifei Fan, Yaping Liu
Summary: miR-29c-3p is overexpressed in benign and malignant ovarian carcinoma and is associated with poor prognosis. Its overexpression modulates tumorigenesis in ovarian cancer cells, including epithelial-mesenchymal transition, proliferation, migration, and invasion, through the regulation of DNMT3A, TET1, and HBP1. miR-29c-3p may serve as a potential biomarker for clinical diagnosis or co-diagnosis of ovarian carcinoma.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Haiyan Zhao, Fangfang Bi, Mengyuan Li, Yuhan Diao, Chen Zhang
Summary: This study confirmed the tumor suppressor effect of RNF180 on ovarian cancer, elucidated the mechanism of the molecule network related to RNF180 and IPO4 in ovarian cancer, and identified a new therapeutic target for ovarian cancer.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chu Chen, Guanhua Xu, Jiajia Chen, Chunshuai Wu, Jinlong Zhang, Jiawei Jiang, Hongxiang Hong, Zhiming Cui
Summary: This study investigated the role of transcription factor FoxO1 in facet joint osteoarthritis (FJOA) and found that FoxO1 deletion led to severe osteoarthritic changes. Transcriptome sequencing and bioinformatics analysis identified differentially expressed genes (DEGs) and potential key contributors to FJOA. Additionally, over-expression of certain genes and inhibition of others were shown to counteract the impairments caused by FoxO1 deletion in chondrocyte migration and extracellular matrix synthesis. These findings help unravel the molecular mechanisms underlying FJOA and open up promising therapeutic avenues for its treatment.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Wen Deng, Ru Chen, Situ Xiong, Jianqiang Nie, Hailang Yang, Ming Jiang, Bing Hu, Xiaoqiang Liu, Bin Fu
Summary: This study demonstrates that circFSCN1 is upregulated in bladder cancer and associated with cancer-specific survival. CircFSCN1 promotes tumor progression and epithelial-mesenchymal transition in bladder cancer through enhancing MDM2-mediated silencing of p53 by sponging miR-145-5p.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Jun Wu, Weibin Hu, Wenhui Yang, Yihao Long, Kaizhao Chen, Fugui Li, Xiaodong Ma, Xun Li
Summary: Cholesterol biosynthesis and metabolism play critical roles in tumor development and microenvironmental conditions. Squalene Epoxidase (SQLE), the second rate-limiting enzyme in cholesterol synthesis, is found to be uniquely expressed in various cancers, and its expression level is closely associated with tumor mutation burden and microsatellite instability. SQLE expression is negatively correlated with immune cell infiltration. Inhibition of SQLE alters the immune response in the tumor microenvironment. Furthermore, protein metabolism and translation are identified as main binding factors with SQLE.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zhihong Zhang, Mingyue Li, Yi Tai, Yue Xing, Hongxiang Zuo, Xuejun Jin, Juan Ma
Summary: ZNF70 plays an important role in colitis-associated colorectal cancer (CAC) by regulating macrophages IL-1 beta secretion to promote HCT116 proliferation. It may serve as a promising target for treating CAC.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zenghong Wu, Gangping Li, Weijun Wang, Kun Zhang, Mengke Fan, Yu Jin, Rong Lin
Summary: This study comprehensively explored the role of immune checkpoints and tumor microenvironment in gastric cancer patients based on genomic data. It constructed an ICIs signature and ICI score to evaluate patient prognosis and heterogeneity.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Yantong Wan, Jieshu Zhou, Panpan Zhang, Xuemei Lin, Hao Li
Summary: This study found that Rac1 plays a role in astrocyte activation and attenuates chronic inflammatory pain by blocking the phosphorylation of NLRP3 inflammasome and NF-kappa B.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zhen Wang, Diankun She, Lei Liu, Xianming Hua, Hao Zhu, Lingfeng Yu, Han Wang, Yan Zhu, Gentao Fan, Yicun Wang, Meng Xu, Guangxin Zhou
Summary: Circular RNAs (circRNAs) are non-coding RNAs that play a role in the regulation of various cancers, including osteosarcoma (OS). This study identified circSATB2 as a highly expressed circRNA in OS tissues and cell lines, and demonstrated its involvement in promoting OS proliferation and migration. Mechanistically, circSATB2 was found to regulate the progression of OS by sponging miR-661 and FUS to regulate ZNFX1 mRNA. These findings suggest that circSATB2 could serve as a prognostic marker and therapeutic target for osteosarcoma.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Kenichi Ogata, Masafumi Moriyama, Tatsuya Kawado, Hiroki Yoshioka, Aiko Yano, Mayu Matsumura-Kawashima, Seiji Nakamura, Shintaro Kawano
Summary: This study found that extracellular vesicles released by induced pluripotent stem cells can reduce inflammatory cell infiltration, increase saliva volume, and decrease the production of antibodies associated with Sjogren's syndrome in a mouse model. The let-7 family in these vesicles may suppress the expression of TLR4 and NF-kappa B, which leads to the inhibition of pro-inflammatory cytokine production through the MAPK pathway.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Mikayla R. Erdelsky, Sarah A. Groves, Charmi Shah, Samantha B. Delios, M. Bibiana Umana, Donald H. Maurice
Summary: Recent evidence suggests that cAMP signaling within the primary cilium plays a crucial role in promoting adipogenic differentiation of 3T3-L1 preadipocytes. In this study, the researchers identified the specific cAMP phosphodiesterases expressed by these cells and found that inhibition of PDE4 promotes FFAR4-mediated adipogenesis. This work could potentially lead to the discovery of more targeted therapeutic approaches for controlling adipogenesis and differentiation of other stem cells.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chun-Hui Liu, Jun-Jie Zhang, Qian-Jin Zhang, Yang Dong, Zhen-Duo Shi, Si-Hao Hong, Hou-Guang He, Wei Wu, Cong-Hui Han, Lin Hao
Summary: Bladder cancer, the most common malignant tumor in the urinary system, is associated with significantly up-regulated expression of P3H4, which is regulated by METTL3 and plays a crucial role in the proliferation, metastasis, and EMT progression of bladder cancer. Targeting this METTL3-P3H4 pathway may serve as a potential therapeutic strategy for bladder cancer.
CELLULAR SIGNALLING
(2024)