期刊
CELLULAR SIGNALLING
卷 24, 期 1, 页码 282-289出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cellsig.2011.09.013
关键词
SPC; Fyn; RhoA; Rho-kinase; Stress fiber
类别
资金
- Ministry of Education, Science, Sports and Culture of Japan
- Center for Gene Research (Yamaguchi University)
- Grants-in-Aid for Scientific Research [10J04493, 23659113, 23380077, 22500364, 22580131] Funding Source: KAKEN
Sphingosylphosphorylcholine (SPC), a bioactive sphingolipid, has recently been reported to modulate actin cytoskeleton rearrangement. We have previously demonstrated Fyn tyrosine kinase is involved in SPC-induced actin stress fiber formation in fibroblasts. However, Fyn-dependent signaling pathway remains to be elucidated. The present study demonstrates that RhoA-ROCK signaling downstream of Fyn controls stress fiber formation in SPC-treated fibroblasts. Here, we found that SPC-induced stress fiber formation was inhibited by C3 transferase, dominant negative RhoA or ROCK SPC activated RhoA, which was blocked by pharmacological inhibition of Fyn activity or dominant negative Fyn. Constitutively active Fyn (ca-Fyn) stimulated stress fiber formation and localized with F-actin at the both ends of stress fibers, both of which were prevented by Fyn translocation inhibitor eicosapentaenoic acid (EPA). In contrast, inhibition of ROCK abolished only the formation of stress fibers, without affecting the localization of ca-Fyn. These results allow the identification of the molecular events downstream SPC in stress fiber formation for a better understanding of stress fiber formation involving Fyn. (C) 2011 Elsevier Inc. All rights reserved.
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