期刊
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
卷 49, 期 2, 页码 728-742出版社
KARGER
DOI: 10.1159/000493038
关键词
Arctigenin; Arrhythmias; Ischemia reperfusion; Oxidative stress; Nrf2 signaling
资金
- National Natural Science Foundation of China [81770407, 31171097, 31271223, 31671184, 2012CB518200, 81603676]
- Grant of Science and Technology Activities for Excellent Returned Chinese Scholars [C2015005004]
- Natural Science Foundation of Hebei Province [C2018206277, H2017206262, C2014206419]
- Research Project of Science and Technology of High School of Hebei Province [ZD2015005, ZD2015007, ZD2016002]
- High-level Talent Support Project of Hebei Province [A2017005070]
Background/Aims: Arctigenin (ATG) has been shown to possess anti-inflammatory, immunemodulatory, anti-viral, anti-microbial, anti-carcinogenic, vasodilatory and anti-platelet-aggregation properties. However, the protective role of ATG in prevention of arrhythmias induced by myocardial ischemia/reperfusion is unknown. The aim of this study was to investigate the anti-arrhythmia effect of ATG in an ischemia/reperfusion injured rat heart model and explore the related mechanisms. Methods: Rats were randomly exposed to sham operation, myocardial ischemia/reperfusion (MI/R) alone, ATG+ MI/R, pretreated with ATG in low (12.5 mg/kg/day), medium (50 mg/kg/day) and high dose (200 mg/kg/day), respectively. Ventricular arrhythmias were assessed. The activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and the level of malondialdehyde (MDA) in myocardial tissue were determined by chemical analysis. Results: Compared to MI/R, rats pretreated with ATG in doses of 50 mg/kg/day and 200 mg/kg/day showed significantly reduced incidence and duration of ventricular fibrillation, ventricular tachycardia and ventricular ectopic beat (VEB), and decreased the arrhythmia score during the 30-min ischemia. Incidence and duration of ventricular tachycardia, infarction size and arrhythmia scores in these groups were significantly decreased during the 120-min reperfusion. No ventricular fibrillation occurred during the period of reperfusion. Rats pretreated with ATG in doses of 50 mg/kg/day and 200 mg/kg/day markedly enhanced the activities of antioxidant enzymes SOD and GSH-Px, reduced the level of MDA. No differences were observed between the group pretreated with a low dose of ATG and the sham group. Administration of ATG significantly increased the expression of antioxidant stress protein Nrf2, Trx1 and Nox1. Conclusion: Our data suggested that ATG plays anti-arrhythmia role in ischemia/reperfusion injury, which is probably associated with attenuating oxidative stress by Nrf2 signaling pathway. (C) 2018 The Author(s) Published by S. Karger AG, Basel.
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