期刊
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
卷 32, 期 5, 页码 1342-1353出版社
KARGER
DOI: 10.1159/000354532
关键词
Arctigenin; Arrhythmia; Ion channel; Patch clamp; Aconitine
资金
- National Nature Science Fund (NNSF) of China [81173638, 81373506]
- Ministry of Education Doctoral Fund [20120031110042]
- Key Teacher Training Project of Heilongjiang Province [1155G61]
- Chinese Postdoctoral Fund [20080440687]
Background/Aims: Arctigenin possesses biological activities, but its underlying mechanisms at the cellular and ion channel levels are not completely understood. Therefore, the present study was designed to identify the anti-arrhythmia effect of arctigenin in vivo, as well as its cellular targets and mechanisms. Methods: A rat arrhythmia model was established via continuous aconitine infusion, and the onset times of ventricular premature contraction, ventricular tachycardia and death were recorded. The Action Potential Duration (APD), sodium current (I-Na), L-type calcium current (I-Ca,I- (L)) and transient outward potassium current (I-to) were measured and analysed using a patch-clamp recording technique in normal rat cardiomyocytes and myocytes of arrhythmia aconitine-induced by. Results: Arctigenin significantly delayed the arrhythmia onset in the aconitine-induced rat model. The 50% and 90% repolarisations (APD(50) and APD(90)) were shortened by 100 mu M arctigenin; the arctigenin dose also inhibited the prolongation of APD(50) and APD(90) caused by 1 mu M aconitine. Arctigenin inhibited I-Na and I-Ca,I-L and attenuated the aconitine-increased I-Na and I-Ca,I-L by accelerating the activation process and delaying the inactivation process. Arctigenin enhanced I-to by facilitating the activation process and delaying the inactivation process, and recoverd the decreased Ito induced by aconitine. Conclusions: Arctigenin has displayed anti-arrhythmia effects, both in vivo and in vitro. In the context of electrophysiology, I-Na, I-Ca,I- L, and I-to may be multiple targets of arctigenin, leading to its antiarrhythmic effect. Copyright (C) 2013 S. Karger AG, Basel
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