期刊
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
卷 30, 期 2, 页码 347-358出版社
KARGER
DOI: 10.1159/000339069
关键词
Monoacylglycerol lipase; Cannabinoid; beta-cells; Human Islets; Insulin Secretion
资金
- Diabetes UK [RD06/0003201, RD07/0003510]
- Henry Lester Trust
- Novo Nordisk UK Research Foundation
- National Council of Science and Technology (CONACYT, Mexico) [00166231]
- PASPA from the DGAPA-UNAM, Mexico
Elements of the endocannabinoid system (ECS) are expressed by islet endocrine cells and activation of CB1 and CB2 cannabinoid receptors regulates insulin secretion from mouse and human beta-cells. The current study aimed to investigate the expression and function, in mouse and human beta-cells, of monoacylglycerol lipase (MGL), an enzyme that facilitates degradation of the endocannabinoid 2-arachidonoylglycerol (2-AG). We found that MGL mRNA is expressed by MIN6 beta-cells, mouse islets, human islets and enriched human islet beta-cells, and immunohistochemistry indicated that MGL localisation in human islets is consistent with its expression by some beta- and - alpha-cells. Blockade of MGL activity with the pharmacological inhibitor URB602 led to increased [Ca2+](i) and enhanced insulin secretion from MIN6 beta-cells, and MGL inhibition also elevated insulin and glucagon secretion from isolated human islets in vitro. These data imply a stimulatory role for endogenous 2-AG in islets that is amplified when its degradation is blocked. Copyright (C) 2012 S. Karger AG, Basel
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据