Article
Microbiology
Matthew A. Hulverson, Samantha A. Michaels, Jin Woo Lee, Karen L. Wendt, Linh T. Tran, Ryan Choi, Wesley C. Van Voorhis, Robert H. Cichewicz, Kayode K. Ojo
Summary: In this study, natural products and fungal extracts were screened for their ability to inhibit the growth of Giardia lamblia, leading to the discovery of novel chemical scaffolds that can potentially be developed into effective antigiardial agents.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Anna Alexanova, Emma Raitoharju, Joona Valtonen, Katriina Aalto-Setala, Leena E. Viiri
Summary: Metabolic dysfunction, driven by altered liver function, predisposes to coronary artery disease (CAD), but the role of liver in vulnerable atherosclerotic plaque development remains unclear.
SCIENTIFIC REPORTS
(2023)
Review
Cell Biology
Hongzhen Chen, Dewei Shang, Yuguan Wen, Chao Liang
Summary: Traditionally viewed as a structural organ, bone is now recognized as an endocrine organ that regulates metabolism and brain development. Abnormal bone metabolism is linked to neurological disorders. Various bone-derived modulators and bone marrow-derived cells play roles in brain function and neurological disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Jan Philipp Schuette, Mailin-Christin Manke, Katherina Hemmen, Patrick Muenzer, Barbara F. Schoerg, Gustavo Campos Ramos, Michaela Pogoda, Valerie Dicenta, Sabrina H. L. Hoffmann, Juergen Pinnecker, Ferdinand Kollotzek, Monika Zdanyte, Karin A. L. Mueller, Yogesh Singh, Andreas F. Mack, Bernd Pichler, Florian Lang, Bernhard Nieswandt, Meinrad Gawaz, Katrin G. Heinze, Nicolas Casadei, Oliver Borst
Summary: Platelet-derived miRNAs play a critical role in myocardial inflammation and structural remodeling in response to myocardial ischemia/reperfusion (I/R). In a mouse model with a Dicer gene deletion, disrupted miRNA processing machinery in platelets led to increased myocardial inflammation, impaired angiogenesis, accelerated cardiac fibrosis, and larger infarct size. These findings highlight the importance of platelet-derived miRNA in regulating cellular processes following myocardial I/R.
CIRCULATION RESEARCH
(2023)
Article
Multidisciplinary Sciences
Ayla Orang, Saira R. Ali, Janni Petersen, Ross A. McKinnon, Amanda L. Aloia, Michael Z. Michael
Summary: Metformin inhibits oxidative phosphorylation in colorectal cancer cells and miRNAs can sensitize these cells to its anti-proliferative effects through modulation of proliferation, apoptosis, cell cycle arrest, and metabolic disruption. Investigation of miRNAs and metformin on CRC cell metabolism can help identify relevant therapeutic targets and design innovative treatment strategies.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Anna Valentino, Stefania Leuci, Umberto Galderisi, Gianrico Spagnuolo, Michele Davide Mignogna, Gianfranco Peluso, Anna Calarco
Summary: We have found a different miRNA expression profile in plasma-derived exosomes (P-EVs) from MPV patients compared to healthy controls, with a significant downregulation of miR-148a-3p in MPV patients. Bioinformatics analysis predicted MMP7 as a potential target of miR-148a-3p. In vitro experiments showed that treatment with Dsg3 autoantibodies downregulated miR-148a-3p and upregulated MMP7 expression, leading to cell dissociation, which was prevented by overexpression of miR-148a-3p.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
H. N. Gao, F. Z. Ren, P. C. Wen, L. X. Xie, R. Wang, Z. N. Yang, Y. X. Li
Summary: This study investigated the effects of yak and cow milk-derived exosomal miRNAs on the barrier function of intestinal epithelial cells (IEC) under hypoxic conditions. It was found that bta-rnift-34a in yak milk exosomes played a crucial role in alleviating hypoxic injury to IEC-6 and increasing cell survival. Additionally, the study shed light on the regulatory relationship between bta-miRNA-34a and the hypoxia-inducible factor/apoptosis signaling pathway, providing insights into the mechanisms by which miRNAs promote survival of intestinal epithelial cells under stress.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Engineering, Biomedical
Hongtao Xu, Yanlun Zhu, Allen Wei-Ting Hsiao, Jiankun Xu, Wenxue Tong, Liang Chang, Xuerao Zhang, Yi-Fan Chen, Jie Li, Wei Chen, Yingze Zhang, Hon Fai Chan, Chien-Wei Lee
Summary: The use of MSC-EVs boosted with bioactive glasses (EVB) shows great potential in treating tendon injuries by promoting angiogenesis and enhancing anti-inflammatory responses of M2 macrophages. EVB can significantly improve the biomechanical properties and promote functional regeneration of the repaired tendon.
Article
Multidisciplinary Sciences
Jing Zhou, Miguel Flores-Bellver, Jianbo Pan, Alberto Benito-Martin, Cui Shi, Onyekwere Onwumere, Jason Mighty, Jiang Qian, Xiufeng Zhong, Tasmim Hogue, Baffour Amponsah-Antwi, Linda Einbond, Rajendra Gharbaran, Hao Wu, Bo-Juen Chen, Zhiliang Zheng, Tatyana Tchaikovskaya, Xusheng Zhang, Hector Peinado, Maria Valeria Canto-Soler, Stephen Redenti
Summary: Research has shown that three-dimensional retinal tissue derived from human induced pluripotent stem cells release exosomes and microvesicles containing small noncoding RNA cargo. The miRNA cargo of these EVs correlates with Gene Ontology pathways relevant to specific developmental stages of human retina development.
SCIENTIFIC REPORTS
(2021)
Article
Medicine, Research & Experimental
Iacopo Gesmundo, Barbara Pardini, Eleonora Gargantini, Giacomo Gamba, Giovanni Birolo, Alessandro Fanciulli, Dana Banfi, Noemi Congiusta, Enrica Favaro, Maria Chiara Deregibus, Gabriele Togliatto, Gaia Zocaro, Maria Felice Brizzi, Raul M. Luque, Justo P. Castano, Maria Alessandra Bocchiotti, Simone Arolfo, Stefania Bruno, Rita Nano, Mario Morino, Lorenzo Piemonti, Huy Ong, Giuseppe Matullo, Juan M. Falcon-Perez, Ezio Ghigo, Giovanni Camussi, Riccarda Granata
Summary: Healthy adipocyte-derived EVs are beneficial for beta cell survival and function, while EVs from inflamed adipocytes cause beta cell death and dysfunction. Lean adipocyte-derived EVs have similar beneficial effects, whereas EVs from obese adipose tissue are harmful for beta cells. Differential expression of miRNAs and alterations in signaling pathways and gene expression in recipient beta cells were observed, suggesting that adipocyte-derived EVs may influence beta cell fate and function depending on the physiological and pathological state of adipose tissue.
Article
Cardiac & Cardiovascular Systems
Xiaofei Luo, Chengcheng Hang, Ziming Zhang, Kaixing Le, Yuhan Ying, Ying Lv, Lingling Yan, Yajie Huang, Lixia Ye, Xuefeng Xu, Ying Zhong, Lizhong Du
Summary: In this study, the researchers discovered that the FoxM1 signaling pathway regulates the function of PASMCs through the transmission of exosomal miRNAs from PVECs. This finding suggests that FoxM1 may serve as a potential therapeutic target for IUGR-induced PH.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2022)
Article
Biochemistry & Molecular Biology
Pei Jiang, Xiangyu Ma, Shanying Han, Leyao Ma, Jingru Ai, Leilei Wu, Yuan Zhang, Hairong Xiao, Mengyao Tian, W. Andy Tao, Shasha Zhang, Renjie Chai
Summary: This study discovers the existence of sEVs in the cochlea and reveals their importance in the normal development of the auditory system.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Oncology
Junlong Zhuang, Lan Shen, Meiqian Li, Jingya Sun, Jiange Hao, Jiaxuan Li, Zhen Zhu, Shuning Ge, Dianzheng Zhang, Hongqian Guo, Ruimin Huang, Jun Yan
Summary: Cancer-associated fibroblasts (CAF) promote cancer stemness and chemoresistance in urothelial bladder cancer (UBC) by overexpressing miR-146a-5p. MiR-146a-5p also enhances UBC stemness and chemoresistance by targeting ARID1A and PRKAA2 mRNA in UBC cells. Elevated levels of exosomal miR-146a-5p in UBC patients' serum are associated with tumor stage and relapse risk.
Article
Oncology
Dorian Forte, Martina Barone, Cristina Morsiani, Giorgia Simonetti, Francesco Fabbri, Samantha Bruno, Erika Bandini, Daria Sollazzo, Salvatore Collura, Maria Chiara Deregibus, Giuseppe Auteri, Emanuela Ottaviani, Nicola Vianelli, Giovanni Camussi, Claudio Franceschi, Miriam Capri, Francesca Palandri, Michele Cavo, Lucia Catani
Summary: In this study, it was found that the absolute numbers of circulating CD34(+) cells were significantly increased in triple-negative (TN) myelofibrosis patients. These cells displayed in vitro defective functions and were unresponsive to the inflammatory microenvironment. Furthermore, the circulating extracellular vesicles (EVs) from TN patients were found to promote the survival of TN CD34(+) cells, suggesting a distinct EV-driven signal promoting malignant hemopoiesis in TN patients. The distinct gene expression profiling analysis and microRNA signature in TN patients may provide insights for novel therapeutic approaches for this rare form of myelofibrosis.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Weizhi Yu, Zhi Lin, Christina M. Woo, Jeremy M. Baskin
Summary: The study uses an ethanol surrogate to generate lipid reporters for visualizing and studying the interactions of phosphatidylethanol (PEth) with proteins in cells. Analysis reveals potential interactors of phosphatidyl alcohols at various membrane locations, with one interaction involving the transmembrane protein basigin/CD147 being validated. This provides a comprehensive view of the molecular interactions of phosphatidyl alcohols with the cellular proteome and suggests further research to link these interactions to potential pathophysiological roles of PEth.
ACS CHEMICAL BIOLOGY
(2022)