期刊
CELLULAR MICROBIOLOGY
卷 13, 期 7, 页码 967-977出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1462-5822.2011.01592.x
关键词
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资金
- United States-Israel Binational Science Foundation [2007350]
- Marie Curie International Reintegration Grant (IRG) [203675]
- German Israel Foundation [[997/2008]
- Israel Science Foundation
- Jacob and Lena Joels Memorial Foundation
- Weizmann Institute of Science
- Gerhardt M.J. Schmidt Minerva Center for Supramolecular Architecture
- Harold Perlman family
- Direct For Computer & Info Scie & Enginr
- Division of Computing and Communication Foundations [2007350] Funding Source: National Science Foundation
The deadliest form of human malaria is caused by the protozoan parasite Plasmodium falciparum. The complex life cycle of this parasite is associated with tight transcriptional regulation of gene expression. Nuclear positioning and chromatin dynamics may play an important role in regulating P. falciparum virulence genes. We have applied an emerging technique of electron microscopy to construct a 3D model of the parasite nucleus at distinct stages of development within the infected red blood cell. We have followed the distribution of nuclear pores and chromatin throughout the intraerythrocytic cycle, and have found a striking coupling between the distributions of nuclear pores and chromatin organization. Pore dynamics involve clustering, biogenesis, and division among daughter cells, while chromatin undergoes stage-dependent changes in packaging. Dramatic changes in heterochromatin distribution coincide with a previously identified transition in gene expression and nucleosome positioning during the mid-to-late schizont phase. We also found a correlation between euchromatin positioning at the nuclear envelope and the local distribution of nuclear pores, as well as a dynamic nuclear polarity during schizogony. These results suggest that cyclic patterns in gene expression during parasite development correlate with gross changes in cellular and nuclear architecture.
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