Article
Biochemistry & Molecular Biology
Vien Q. T. Ho, Mark K. Rong, Eva Habjan, Samantha D. Bommer, Thang V. Pham, Sander R. Piersma, Wilbert Bitter, Eelco Ruijter, Alexander Speer
Summary: In this study, a 1,2,4-oxadiazole derivative was found to inhibit the secretion of active lipase LipY by the ESX-5 secretion system. Other ESX-5 substrates were even more abundantly secreted in the presence of several 1,2,4-oxadiazoles. These compounds significantly reduced bacterial burden in zebrafish models.
Article
Multidisciplinary Sciences
Rachel M. Cronin, Micah J. Ferrell, Clare W. Cahir, Matthew M. Champion, Patricia A. Champion
Summary: ESX-1 substrates have differential requirements in secretion and virulence in M. marinum, which can be quantitatively measured by isobaric-tagged proteomics. Our study provides important insights into the secretion mechanism of ESX-1 substrates and reveals their discrete roles in function and virulence of M. marinum.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Merel P. M. Damen, Aniek S. Meijers, Esther M. Keizer, Sander R. Piersma, Connie R. Jimenez, Coenraad P. Kuijl, Wilbert Bitter, Edith N. G. Houben
Summary: Pathogenic mycobacteria utilize the ESX-1 secretion system to mediate intracellular survival, but determining the responsible ESX-1 substrate is challenging due to complex secretion dependencies. This study reveals the critical role of the ESX-1 substrate PPE68 in mediating the secretion of ESX-1 substrates in Mycobacterium marinum, providing insights into the functional understanding of T7SSs and the virulence of Mycobacterium tuberculosis.
Article
Microbiology
Kevin G. Sanchez, Rebecca J. Prest, Kathleen R. Nicholson, Konstantin V. Korotkov, Patricia A. Champion
Summary: Pathogenic mycobacteria, including Mycobacterium tuberculosis, rely on the ESX-1 secretion system to survive and interact with the host. In this study, the researchers investigated the regulation of the ESX-1 system by the transcription factor EspM. They identified domains required for EspM to form multimers and bind DNA, providing insights into its mechanism of action.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Multidisciplinary Sciences
Yuchen Wang, Yuting Tang, Chen Lin, Junli Zhang, Juntao Mai, Jun Jiang, Xiaoxiao Gao, Yao Li, Guoping Zhao, Lu Zhang, Jun Liu
Summary: The ESX-4 system in Mycobacterium marinum does not secrete its cognate substrates, but the deletion of eccC4, an essential component of ESX-4, resulted in elevated secretion of protein substrates of ESX-1 and ESX-5, leading to enhanced phagocytosis by macrophages.
Article
Microbiology
Marion Lagune, Vincent Le Moigne, Matt D. Johansen, Flor Vasquez Sotomayor, Wassim Daher, Cecile Petit, Gina Cosentino, Laura Paulowski, Thomas Gutsmann, Matthias Wilmanns, Florian P. Maurer, Jean-Louis Herrmann, Fabienne Girard-Misguich, Laurent Kremer
Summary: ESX type VII secretion systems play an important role in pathogenicity, nutrient uptake and conjugation in mycobacteria. EsxU and EsxT are substrates of ESX-4 and form a stable heterodimer that permeabilizes artificial membranes. Deletion of the esxUT genes increases the virulence of M. abscessus in animal models.
Article
Multidisciplinary Sciences
Uday Tak, Terje Dokland, Michael Niederweis
Summary: The tuberculosis necrotizing toxin (TNT) is secreted by Mycobacterium tuberculosis to kill host cells, with proteins EsxE and EsxF forming membrane-spanning hetero-oligomeric pores that are essential for TNT secretion.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
B. Izquierdo Lafuente, R. Ummels, C. Kuijl, W. Bitter, A. Speer
Summary: CpnT, a potent secreted toxin of Mycobacterium tuberculosis, is exclusively secreted by the ESX-5 system in bacterial culture but relies on intact ESX-1, ESX-4, and ESX-5 systems during infection studies. This highlights the intricate interplay of three different secretion systems in secreting one substrate during infection.
Article
Chemistry, Physical
Bing Li, Siyuan He, Zhili Tan, Anqi Li, Junsheng Fan, Lan Zhao, Zhemin Zhang, Haiqing Chu
Summary: This study reveals that impaired ESX-3 severely restricts the growth of M. abscessus under iron-limited conditions and induces persistence to bedaquiline. The deficiency of iron due to impaired ESX-3 suppresses succinate dehydrogenase activity, dysregulates the tricarboxylic acid cycle, and inactivates bedaquiline. Moreover, the regulator MtrA can bind ESX-3 and promote the survival of M. abscessus.
Article
Multidisciplinary Sciences
Lin Wang, Emmanuel Asare, Amol C. Shetty, Freddy Sanchez-Tumbaco, Megan R. Edwards, Rajagopalan Saranathan, Brian Weinrick, Jiayong Xu, Bing Chen, Angele Benard, Gordon Dougan, Daisy W. Leung, Gaya K. Amarasinghe, John Chan, Christopher F. Basler, William R. Jacobs, JoAnn M. Tufariello
Summary: Mycobacterium tuberculosis possesses five type VII secretion systems, and the deletion of a specific substrate gene in one of these systems leads to the need for iron supplementation for growth and a significant decrease in virulence. This study identified the mechanisms behind the suppression of this attenuated phenotype, which involves the overexpression of a substrate in a paralogous region lacking the secretion apparatus genes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
Qinglong Guo, Jing Bi, Honghai Wang, Xuelian Zhang
Summary: EsxA, secreted by the ESX-1 secretion system, is a major virulence factor of Mycobacterium tuberculosis. EspC, an ESX-1 substrate, triggers ER stress-mediated apoptosis in macrophages, leading to inflammation, Ca2+ release, and bacterial survival. Understanding the role of EspC in Mtb-host interactions sheds light on complex host-pathogen interactions and mechanisms underlying pathogenesis mediated by the ESX-1 system.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Chemistry, Medicinal
Kylee Drever, Ze Long Lim, Slim Zriba, Jeffrey M. Chen
Summary: This study provides the first evidence of the importance of mycobacterial proteostasis to ESX-1 secretion system and identifies novel vulnerabilities in the ESX-1 system for potential anti-TB drug targets.
ACS INFECTIOUS DISEASES
(2021)
Article
Microbiology
Glennon Bythrow, Manal F. Farhat, Keith Levendosky, Poornima Mohandas, Gabrielle A. Germain, Barney Yoo, Luis E. N. Quadri
Summary: The opportunistic pathogen Mab is associated with chronic pulmonary disease. Understanding its biology can lead to better therapeutics. This study reveals a functional link between the ESX-3 secretion system and an iron uptake system, and identifies a potentially pathogenic siderophore.
Article
Microbiology
November Sankey, Haley Merrick, Padam Singh, Janet Rogers, Amit Reddi, Steven D. D. Hartson, Avishek Mitra
Summary: Mycobacterium tuberculosis (Mtb) is a successful pathogen that can overcome iron limitation by utilizing heme in the human host. The ESX-4 type VII secretion system (T7SS) and two specific proteins, Rv0125 and Rv1085c, play essential roles in heme uptake. Additionally, the alternative sigma factor SigM is a global regulator of core heme utilization genes. Furthermore, some mycobacterial PPE proteins have been shown to form channels in the outer mycomembrane for nutrient acquisition.
Article
Food Science & Technology
M. C. Rojo, F. N. Arroyo Lopez, M. C. Lerena, L. Mercado, A. Torres, M. Combina
Article
Food Science & Technology
Y. Paola Maturano, M. Victoria Mestre, Braulio Esteve-Zarzoso, Maria Cristina Nally, Maria Cecilia Lerena, Maria Eugenia Toro, Fabio Vazquez, Mariana Combina
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2015)
Review
Cell Biology
Maria C. Lerena, Cristina L. Vazquez, Maria I. Colombo
CELLULAR MICROBIOLOGY
(2010)
Article
Biotechnology & Applied Microbiology
M. C. Rojo, F. N. Arroyo Lopez, M. C. Lerena, L. Mercado, A. Torres, M. Combina
Article
Food Science & Technology
Y. P. Maturano, M. C. Lerena, M. V. Mestre, L. F. Casassa, M. E. Toro, F. Vazquez, L. Mercado, M. Combina
LWT-FOOD SCIENCE AND TECHNOLOGY
(2018)
Article
Food Science & Technology
M. C. Rojo, M. Cristiani, N. Szerman, M. L. Gonzalez, M. C. Lerena, L. A. Mercado, M. Combina
FOOD AND BIOPROCESS TECHNOLOGY
(2019)
Article
Food Science & Technology
A. S. Vargas-Trinidad, M. C. Lerena, J. Alonso-del-Real, B. Esteve-Zarzoso, L. A. Mercado, A. Mas, A. Querol, M. Combina
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Magali Lucia Gonzalez, Maria Elena Sturm, Maria Cecilia Lerena, Maria Cecilia Rojo, Selva Valeria Chimeno, Mariana Combina, Laura Analia Mercado
Article
Food Science & Technology
M. C. Lerena, M. C. Rojo, S. Sari, L. A. Mercado, S. Krieger-Weber, M. Combina
SOUTH AFRICAN JOURNAL OF ENOLOGY AND VITICULTURE
(2016)