4.5 Article

A single cell tracking system in real-time

期刊

CELLULAR IMMUNOLOGY
卷 265, 期 1, 页码 44-49

出版社

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cellimm.2010.07.001

关键词

Real-time; Cell tracking; Chemotaxis

资金

  1. Ministry of Commerce, Industry and Energy (MOCIE), Korea
  2. Ministry of Education, Science and Technology (MEST), Korea

向作者/读者索取更多资源

We describe here a novel real-time cell tracking system which can measure cell migration routes under cell culture condition. This system includes a mini incubator which controls temperature and CO2 gas flow and a PDMS (polydimethylsiloxane) chip for chemotaxis measurement. The main differences from previous ones are real-timely long-term (>= 24 h) tracking for single cell quantitatively, simple and inexpensive constitution of optical parts for illumination and imaging, and compatible to commercial well plate. The tracking principle is to trace cell images for each 0.2 s by converting the live cell images to binary images of black and white. Migration results of HUVEC and NCI-H23 cells are obtained respectively using this system. The results are single cell path (x, y) during migration, cell size, migration distance, migration speed, real-time pictures and so on. This system is applicable to all kinds of researches related to cell migration such as cell angiogenesis, chemotaxis, and moreover cancer metastasis. (C) 2010 Elsevier Inc. All rights reserved.

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