期刊
CELLULAR IMMUNOLOGY
卷 262, 期 1, 页码 44-51出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cellimm.2009.12.006
关键词
Regulatory B cell; Milk allergy; Non-IgE-mediated food allergy; CD5; Atopic dermatitis; Apoptosis; Interleukin 10
资金
- Korean Ministry of Education, Science and Technology
B cells have regulatory functions in immune responses. Antigen-specific responses of B cell subsets by allergen stimulation ex vivo were examined in milk allergy of late eczematous reactions. Eight milk allergy subjects and 13 milk tolerant subjects were selected by DBPCFC. PBMCs were stimulated by casein ex vivo and stained for B cell subsets using monoclonal antibodies. CD19+ B cells unchanged from 8.7 +/- 3.8% to 8.0 +/- 5.1 % (p = 0.504, n = 8) in the milk allergy group and decreased in the milk tolerant group from 8.5 +/- 12% to 5.0 +/- 1.6% (p = 0.001, n = 13). The fraction of apoptotic B cells in B cells significantly decreased 4.4 +/- 3.1% to 1.3 +/- 0.4% (p=0.027, n=4) in the allergy group and insignificantly increased from 2.8 +/- 0.6% to 5.4 +/- 2.6% (p = 0.059, n = 6) in the milk tolerant group. CD5+ regulatory B1 cell% in B cells decreased in milk allergy subjects from 36.2 +/- 5.0% to 31.0 +/- 5.7% (p = 0.010) and unchanged in milk tolerant subjects from 41.6 +/- 10.2% to 43.8 +/- 10.0% (p = 0.413). IL-10 producing CD19+CD5+ regulatory B cell% in CD19+CD5+ regulatory B cells significantly decreased from 24.9 +/- 6.5% to 13.8 +/- 5.6% (p = 0.002, n = 5) by casein stimulation in milk allergy group and unchanged from 44.8 +/- 11.3% to 43.9 +/- 10.0% (p = 0.297, n = 5) in the milk tolerant group. B cell subset responses to IL-4 and IL-5 were also similar in both groups. B cell subset changes seemed to have diagnostic value. Exact immunologic roles of regulatory CD5+ B1 cells need further investigation. (C) 2010 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据