Article
Cell Biology
Yunmi Ko, Yeon Ho Jeong, Jun Ah Lee
Summary: This study investigated the therapeutic potential of expanded gamma delta T cells against osteosarcoma (OS). The expanded gamma delta T cells exhibited potent cytotoxicity against OS in vitro and may enhance the effect of chemotherapeutic agents. This suggests that gamma delta T cells could be a new treatment strategy for OS, including chemo-immunotherapy.
Review
Chemistry, Multidisciplinary
Bohwa Han, Yeonju Song, Jeehun Park, Junsang Doh
Summary: This review discusses the use of nanomaterials to address the limitations of CAR-T cell therapy, including methods to improve expansion, gene delivery, and enhance antitumor functions. Understanding the biological principles behind various nanomaterials is crucial for their application in ex vivo engineering of T/NK cells.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Oncology
Jeong A. Park, Nai-Kong Cheung
Summary: The study found that using multi-antigen targeting EATs can maintain target antigen specificity and anti-tumor potency, with substantially less cytokine release in the presence of tumor cells compared to mixing multiple BsAbs with unarmed T cells. Dual-armed EATs or multi-armed EATs effectively suppressed tumor growth and were highly efficient in preventing clonal escape.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Chemistry, Multidisciplinary
Stefania Douka, Lisa E. Brandenburg, Cristina Casadidio, Johanna Walther, Bianca Bonetto Moreno Garcia, Jan Spanholtz, Monica Raimo, Wim E. Hennink, Enrico Mastrobattista, Massimiliano Caiazzo
Summary: This study investigates the delivery of mRNA to NK cells using polymeric and lipid nanoparticle formulations. The results show that mRNA-LNPs have a high transfection efficiency and protein expression in NK cells, making them a promising strategy for NK cell therapies.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Biology
Anders Laustsen, Renee M. Van der Sluis, Albert Gris-Oliver, Sabina Sanchez Hernandez, Ena Cemalovic, Hai Q. Tang, Lars Henning Pedersen, Niels Uldbjerg, Martin R. Jakobsen, Rasmus O. Bak
Summary: Researchers have developed a new method for generating pDCs from whole blood, and these cells exhibit the characteristics and functions of pDCs. While TLR7/9 responses significantly decrease when cultured under GMP standards, this can be rescued by adding ascorbic acid.
Article
Oncology
Yaya Chu, Gaurav Nayyar, Susiyan Jiang, Jeremy M. Rosenblum, Patrick Soon-Shiong, Jeffrey T. Safrit, Dean A. Lee, Mitchell S. Cairo
Summary: The study demonstrates that the combination of N-803 with dinutuximab and exPBNK cells significantly enhances the anti-tumor effects against GD2(+) solid tumors, by enhancing NK cell activity and anti-tumor actions, reducing the secretion of various factors from tumor cells, and extending the survival time of mice.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Gastroenterology & Hepatology
Zhouhong Ge, Guoying Zhou, Lucia Campos Carrascosa, Erik Gausvik, Patrick P. C. Boor, Lisanne Noordam, Michael Doukas, Wojciech G. Polak, Turkan Terkivatan, Qiuwei Pan, R. Bart Takkenberg, Joanne Verheij, Joris Erdmann, Jan N. M. IJzermans, Maikel P. Peppelenbosch, Jaco Kraan, Jaap Kwekkeboom, Dave Sprengers
Summary: The study demonstrates that co-blockade of TIGIT/PD1 can improve the functionality of CD8(+) TILs in HCC patients, which could be a promising immune therapeutic strategy for patients who do not respond to single PD1 blockade.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Clinical Neurology
Regina R. Reimann, Martina Puzio, Antonella Rosati, Marc Emmenegger, Bernard L. Schneider, Pamela Valdes, Danzhi Huang, Amedeo Caflisch, Adriano Aguzzi
Summary: The cellular prion protein PrPC mediates neurotoxicity of prions and protein aggregates, but the mechanisms are not well understood. Antibody-derived ligands against PrPC induce neurotoxicity through hydrogen bonding and suppressing this bond prolongs the lives of prion-infected mice, suggesting convergent pathways. A study found that the toxic effects of these ligands require a specific amino acid residue within PrPC, which could be a potential target for preventing prion-related neurodegeneration.
Article
Oncology
Jeong A. Park, Brian H. Santich, Hong Xu, Lawrence G. Lum, Nai-Kong V. Cheung
Summary: T cell-based immunotherapies using BsAb armed T cells provide a safe alternative with improved antitumor potency. EATs release less TNF-alpha and infiltrate tumors faster, achieving robust antitumor responses. The in vivo potency of EAT therapy depends on BsAb dose, cell number, total dose, and treatment schedule intensity. EATs remain effective after cryopreservation, and third-party gamma delta -EATs offer an alternative for autologous T cell sources.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Oncology
Daun Jung, Young Seok Baek, In Jee Lee, Ki Yeon Kim, Heejoo Jang, Sohyun Hwang, Jieun Jung, Yong-wha Moon, Kyung-Soon Park, Yong-Soo Choi, Hee Jung An
Summary: This study focused on ex vivo expansion and cryopreservation of allogeneic NK cells, which showed upregulated genes related to NK cell functions and exhibited potent cytolytic activity against cancer cell lines. The research suggests the potential use of expanded and cryopreserved NK cells as a promising immunotherapy for drug-resistant cancer patients.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Cell Biology
Hiroki Kawano, Yuko Kawano, Chen Yu, Mark W. LaMere, Matthew J. McArthur, Michael W. Becker, Scott W. Ballinger, Satoshi Gojo, Roman A. Eliseev, Laura M. Calvi
Summary: Mitochondrial dysfunction is common in various conditions, and mitochondrial DNA (mtDNA) transfer may be a promising strategy for treatment. In this study, we successfully expanded mouse hematopoietic stem cells (HSCs) ex vivo and observed high engraftment of the donor cells in the host. We also found that HSCs from donor mice could transfer mtDNA to host cells, highlighting the utility of ex vivo expanded HSCs for mitochondrial transfer.
Article
Immunology
Xiyuan Bai, Deepshikha Verma, Cindy Garcia, Ariel Musheyev, Kevin Kim, Lorelenn Fornis, David E. Griffith, Li Li, Nicholas Whittel, Jacob Gadwa, Tamara Ohanjanyan, Matthew J. Eggleston, Manuel Galvan, Brian M. Freed, Diane Ordway, Edward D. Chan
Summary: Cigarette smoke exposure can impair the immune response against Mycobacterium tuberculosis (MTB) infection. In this study, it was found that cigarette smoke-exposed T regulatory cells (Tregs) can exacerbate MTB infection by impairing macrophage function and promoting immunosuppression. This was demonstrated through co-culture experiments and mouse models. These findings suggest that cigarette smoke not only directly affects immune cells, but also indirectly promotes TB infection through the modulation of Tregs.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Roberta Fusco, Giacomo Perazzolo Gallo, Elio Di Bernardo, Valeria D'Alessio, Mattia Ronchetti, Matteo Cadossi, Ruggero Cadossi
Summary: The aim of this document is to provide an overview of gene electrotransfer in ophthalmological disorders. Several electronic databases were considered and studies published between January 1998 and December 2021 were analyzed. The results showed that electrotransfer could be very efficient for plasmid DNA transfection. The optimization of electric field setting seems to be important for future gene therapy technologies in the treatment of human eye diseases.
Article
Neurosciences
Valentin H. Prevost, Andrew Yung, Sarah R. Morris, Irene M. Vavasour, Zahra Samadi-Bahrami, G. R. Wayne Moore, Cornelia Laule, Alex Mackay, Piotr Kozlowski
Summary: The temperature has different effects on unfiltered and filtered ihMT, with MWF decreasing and filtered ihMTR increasing at higher temperatures. The recommendations suggest controlled temperatures close to 37 degrees C for filtered ihMT acquisitions, while ex vivo myelin water imaging should be performed at room temperature.
Article
Engineering, Biomedical
Hsuan-Yu Mu, Chiao-Min Lin, Li-An Chu, Ya-Hui Lin, Ji Li, Chao-Yu Liu, Hsi-Chien Huang, Sheng-Liang Cheng, Tsung-Ying Lee, Hsin Mei Lee, Hsin-Min Chen, Yun-Jen Tsai, Yunching Chen, Jen-Huang Huang
Summary: Combination immunotherapy utilizing a tumor-microenvironment-on-chip (TMoC) has shown potential in addressing challenges of immune checkpoint inhibitor therapy in breast cancer. The TMoC platform allows for accurate evaluation of drug synergies in complex TME conditions, offering valuable insights for drug development and clinical decision-making.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Clinical Neurology
Koji Fujita, Tomoyasu Matsubara, Ryosuke Miyamoto, Hiroyuki Sumikura, Toshiaki Takeuchi, Keiko Maruyama Saladini, Toshitaka Kawarai, Hiroyuki Nodera, Fukashi Udaka, Kodai Kume, Hiroyuki Morino, Hideshi Kawakami, Masato Hasegawa, Ryuji Kaji, Shigeo Murayama, Yuishin Izumi
Article
Cardiac & Cardiovascular Systems
Junya Aoki, Yasuyuki Iguchi, Takao Urabe, Hiroshi Yamagami, Kenichi Todo, Shigeru Fujimoto, Koji Idomari, Nobuyuki Kaneko, Takeshi Iwanaga, Tadashi Terasaki, Ryota Tanaka, Nobuaki Yamamoto, Akira Tsujino, Koichi Nomura, Koji Abe, Masaaki Uno, Yasushi Okada, Hideki Matsuoka, Sen Yamagata, Yasumasa Yamamoto, Toshiro Yonehara, Takeshi Inoue, Yoshiki Yagita, Kazumi Kimura, Hidetaka Mitsumura, Yuji Ueno, Masao Watanabe, Yuki Sakamoto, Shuji Arakawa, Yoshinari Nagakane, Ryota Ishibashi, Yuka Terasawa, Koji Fujita, Kenichi Kashihara, Mutsumi Mitomi, Tatsu Nakano, Kensaku Shibazaki, Oshiki Takao, Yohei Tateishi, Seiji Goto, Yasuhiro Manabe, Naoaki Kanda, Toshihiko Ohashi, Yoko Okada, Ryo Itabashi, Eisuke Furui, Takaaki Takizawa, Masahiro Minami, Yasuhiro Noguchi, Yoshiyuki Kondo, Tesseki Izumi, Hirokuni Sakima, Yasushi Ueno, Junji Kasuya, Naoki Oba
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2019)
Article
Medicine, General & Internal
Keiko Imamura, Yuishin Izumi, Haruhiko Banno, Ryuji Uozumi, Satoshi Morita, Naohiro Egawa, Takashi Ayaki, Makiko Nagai, Kazutoshi Nishiyama, Yasuhiro Watanabe, Ritsuko Hanajima, Ryosuke Oki, Koji Fujita, Naoto Takahashi, Takafumi Ikeda, Akira Shimizu, Akiko Morinaga, Tomoko Hirohashi, Yosuke Fujii, Ryosuke Takahashi, Haruhisa Inoue
Article
Medicine, General & Internal
Yuki Yamamoto, Nobuaki Yamamoto, Koji Fujita, Tatsuya Fukumoto, Nagahisa Murakami, Hideo Mure, Yasuhisa Kanematsu, Yasushi Takagi, Yuishin Izumi
Review
Neurosciences
Hideo Mure, Naoto Toyoda, Ryoma Morigaki, Koji Fujita, Yasushi Takagi
STEREOTACTIC AND FUNCTIONAL NEUROSURGERY
(2020)
Article
Neurosciences
Ryoma Morigaki, Ryosuke Miyamoto, Hideo Mure, Koji Fujita, Taku Matsuda, Yoko Yamamoto, Masahito Nakataki, Tetsuya Okahisa, Yuki Matsumoto, Kazuhisa Miyake, Nobuaki Yamamoto, Ryuji Kaji, Yasushi Takagi, Satoshi Goto
Article
Medicine, General & Internal
Takayuki Kondo, Haruhiko Banno, Taro Okunomiya, Yoko Amino, Kayoko Endo, Akiyoshi Nakakura, Ryuji Uozumi, Akemi Kinoshita, Harue Tada, Satoshi Morita, Hidehiro Ishikawa, Akihiro Shindo, Ken Yasuda, Yosuke Taruno, Takakuni Maki, Takashi Suehiro, Kohji Mori, Manabu Ikeda, Koji Fujita, Yuishin Izumi, Kazutomi Kanemaru, Kenji Ishii, Kazue Shigenobu, Yumiko Kutoku, Yoshihide Sunada, Shinobu Kawakatsu, Shunji Shiota, Toshifumi Watanabe, Osamu Uchikawa, Ryosuke Takahashi, Hidekazu Tomimoto, Haruhisa Inoue
Summary: AD is a common cause of dementia, with PSEN1 gene mutations being a frequent cause. Current treatments for PSEN1-AD are limited, but iPSC-based drug repurposing identified bromocriptine as a therapeutic candidate. A multicentre trial will investigate the safety and efficacy of bromocriptine in PSEN1-AD patients, with primary outcomes focusing on cognitive and psychological function. The study is conducted ethically and results will be disseminated at conferences and published in journals.
Article
Biology
Hiroshi Koyama, Hideo Mure, Ryoma Morigaki, Ryosuke Miyamoto, Kazuhisa Miyake, Taku Matsuda, Koji Fujita, Yuishin Izumi, Ryuji Kaji, Satoshi Goto, Yasushi Takagi
Summary: The study assessed the outcomes of 12 patients with TD who underwent GPi DBS, with an average BFMDRS improvement rate of 75.6% at 1 month postoperatively and 78.0% in the long term. Two patients responded poorly to DBS, which was attributed to longer duration from TD onset to surgery and older age at surgery.
Article
Multidisciplinary Sciences
Naohiro Egawa, Yuishin Izumi, Hidefumi Suzuki, Itaru Tsuge, Koji Fujita, Hitoshi Shimano, Keiichi Izumikawa, Nobuhiro Takahashi, Kayoko Tsukita, Takako Enami, Masahiro Nakamura, Akira Watanabe, Motoko Naitoh, Shigehiko Suzuki, Tsuneyoshi Seki, Kazuhiro Kobayashi, Tatsushi Toda, Ryuji Kaji, Ryosuke Takahashi, Haruhisa Inoue
Summary: This study suggests the association between TDP-43 and dyslipidemia in ALS. Increased TDP-43 inhibits the transcriptional activity of SREBP2, leading to suppression of cholesterol biosynthesis. These findings contribute to a better understanding of the pathological mechanism of ALS.
SCIENTIFIC REPORTS
(2022)
Article
Clinical Neurology
Ryosuke Oki, Yuishin Izumi, Koji Fujita, Ryosuke Miyamoto, Hiroyuki Nodera, Yasutaka Sato, Satoshi Sakaguchi, Hiroshi Nokihara, Kazuaki Kanai, Taiji Tsunemi, Nobutaka Hattori, Yuki Hatanaka, Masahiro Sonoo, Naoki Atsuta, Gen Sobue, Toshio Shimizu, Kazumoto Shibuya, Ken Ikeda, Osamu Kano, Kazuto Nishinaka, Yasuhiro Kojima, Masaya Oda, Kiyonobu Komai, Hitoshi Kikuchi, Nobuo Kohara, Makoto Urushitani, Yoshiaki Nakayama, Hidefumi Ito, Makiko Nagai, Kazutoshi Nishiyama, Daisuke Kuzume, Shun Shimohama, Takayoshi Shimohata, Koji Abe, Tomohiko Ishihara, Osamu Onodera, Sagiri Isose, Nobuyuki Araki, Mitsuya Morita, Kazuyuki Noda, Tatsushi Toda, Hirofumi Maruyama, Hirokazu Furuya, Satoshi Teramukai, Tatsuo Kagimura, Kensuke Noma, Hiroaki Yanagawa, Satoshi Kuwabara, Ryuji Kaji
Summary: This study aimed to validate the efficacy and safety of ultrahigh-dose methylcobalamin for patients with early-stage ALS. The results showed that methylcobalamin was effective in slowing functional decline in ALS patients, and it was safe to use during the 16-week treatment period.
Article
Clinical Neurology
Koji Fukushima, Naoko Takamatsu, Yuki Yamamoto, Hiroki Yamazaki, Takeshi Yoshida, Yusuke Osaki, Shotaro Haji, Koji Fujita, Kazuma Sugie, Yuishin Izumi
Summary: Fasciculation detected in relevant muscles on muscle ultrasonography can contribute to the early diagnosis of ALS. We developed and validated diagnostic models using machine learning for early-stage ALS.
CLINICAL NEUROPHYSIOLOGY
(2022)
Article
Health Care Sciences & Services
Shotaro Haji, Koji Fujita, Ryosuke Oki, Yusuke Osaki, Ryosuke Miyamoto, Hiroyuki Morino, Seiichi Nagano, Naoki Atsuta, Yuki Kanazawa, Yuki Matsumoto, Atsuko Arisawa, Hisashi Kawai, Yasutaka Sato, Satoshi Sakaguchi, Kenta Yagi, Tatsuto Hamatani, Tatsuo Kagimura, Hiroaki Yanagawa, Hideki Mochizuki, Manabu Doyu, Gen Sobue, Masafumi Harada, Yuishin Izumi
Summary: EPI-589, an orally available small molecule, shows promising potential as a therapeutic agent for ALS due to its strong protective effects against oxidative stress. The EPIC-ALS trial aims to assess the safety and tolerability of EPI-589 in ALS patients, as well as its impact on oxidative stress and neuronal damage. The trial began in September 2021 and is expected to be completed in October 2023.
JMIR RESEARCH PROTOCOLS
(2023)
Article
Medicine, General & Internal
Keiko Imamura, Yuishin Izumi, Makiko Nagai, Kazutoshi Nishiyama, Yasuhiro Watanabe, Ritsuko Hanajima, Naohiro Egawa, Takashi Ayaki, Ryosuke Oki, Koji Fujita, Ryuji Uozumi, Akiko Morinaga, Tomoko Hirohashi, Yosuke Fujii, Takuya Yamamoto, Harutsugu Tatebe, Takahiko Tokuda, Naoto Takahashi, Satoshi Morita, Ryosuke Takahashi, Haruhisa Inoue
Summary: This study aimed to evaluate the safety and efficacy of bosutinib in patients with ALS, finding good tolerability in patients with a dose limit of 300mg QD, with a subset of patients showing treatment response to bosutinib, and no ALS-specific adverse events were observed.
Article
Genetics & Heredity
Kohei Muto, Ryosuke Miyamoto, Yuka Terasawa, Yoshimitsu Shimatani, Keijiro Hara, Takumi Kakimoto, Tatsuya Fukumoto, Yusuke Osaki, Koji Fujita, Masafumi Harada, Hisanori Uehara, Yasushi Takagi, Yuishin Izumi
Summary: COL4A1-related disorders are characterized by a higher risk of cerebral hemorrhage, broad phenotypic variations, extracerebral hemorrhages, and the coexistence of neural tumors.
HUMAN GENOME VARIATION
(2021)
Meeting Abstract
Clinical Neurology
Yuishin Izumi, Ryosuke Miyamoto, Koji Fujita, Hiroyuki Sumikura, Yasuhiro Sakashita, Hiroyuki Nodera, Toshitaka Kawarai, Yshihiko Nishida, Shigeo Murayama, Ryuji Kaji
