Article
Medicine, Research & Experimental
Xiao Li, Chongyang Chen, Xu Zhan, Binyao Li, Zaijun Zhang, Shupeng Li, Yongmei Xie, Xiangrong Song, Yuanyuan Shen, Jianjun Liu, Ping Liu, Gong-Ping Liu, Xifei Yang
Summary: The study showed that treatment with R13 significantly slowed the abnormal motor performance of ALS mice, reduced the proliferation of microglia and astrocytes, promoted mitochondrial biogenesis, and increased the survival rate of worms expressing G93A SOD1.
Article
Neurosciences
Biying Yang, Jingrui Pan, Xiao-Ni Zhang, Hongxuan Wang, Lei He, Xiaoming Rong, Xiangpen Li, Ying Peng
Summary: This study found that oxidative stress inhibited iron-dependent lipid peroxidation and promoted redox reactions through the activation of the nuclear factor erythroid 2-related factor 2 (NRF2) signaling pathway, which slowed down the neurodegeneration in motor neurons with ALS. The study demonstrated the importance of glutathione peroxidase 4 (GPX4) in ALS patients with the hSOD1G93A mutation.
NEUROBIOLOGY OF DISEASE
(2023)
Article
Cell Biology
Jie Zhang, An Wen, Wen Chai, Huiting Liang, Chunyan Tang, Weiming Gan, Renshi Xu
Summary: This study systematically analyzed and compared brain proteomic alterations in an ALS mouse model and wild-type mice, revealing important proteins and related cellular components, molecular functions, biological processes, and pathways involved in ALS development. These findings have significant implications for further research on abnormal proteins in the pathogenesis of ALS.
CELL BIOLOGY INTERNATIONAL
(2022)
Article
Neurosciences
Gan Zhang, Rong Liu, Zhaofu Sheng, Yonghe Zhang, Dongsheng Fan
Summary: The expression and function of SIRT1 in the hypothalamus in amyotrophic lateral sclerosis (ALS) is investigated. SIRT1 is found to be overexpressed in the hypothalamus after motor symptom onset and interacts with prepro-orexin, a molecule involved in energy balance and the sleep/wake cycle, in both preclinical and clinical ALS. These findings suggest that SIRT1 might participate in sleep and metabolic changes in ALS, indicating it as a potential new target for ALS treatment.
Article
Biochemistry & Molecular Biology
Tanya S. McDonald, Vinod Kumar, Jenny N. Fung, Trent M. Woodruff, John D. Lee
Summary: The study aimed to elucidate deficits in energy homeostasis in ALS using SOD1(G93A) mouse model. Mid-symptomatic SOD1(G93A) mice showed increased oxygen consumption, faster glucose uptake, disturbed fasting glucose homeostasis, and increased liver glycogen stores despite elevated glucagon concentrations. This suggests that hormonal regulation of glucose concentrations may contribute to disease progression in ALS.
Article
Pharmacology & Pharmacy
Nuria Gaja-Capdevila, Neus Hernandez, Xavier Navarro, Mireia Herrando-Grabulosa
Summary: The study demonstrated that Sig-1R ligands, including three different drugs, can partially alleviate muscle function impairment in ALS patients and increase the number of surviving motor neurons. Furthermore, these drugs showed varying effects in improving muscle function and protecting neuromuscular junctions. Although more research is needed to determine their exact mechanisms of action, Sig-1R ligands have the potential to be promising tools for ALS treatment.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Neurosciences
Siyue Qin, Pan You, Hui Yu, Bo Su
Summary: A decrease in REEP1 expression in SOD1(G93A) mice with ALS was found, and forced expression of REEP1 in the spinal cord improved motor performance, prolonged lifespan, and alleviated neuromuscular synaptic loss, gliosis, and motor neuron loss in the mice. Mechanistically, REEP1 associates with NDUFA4 and plays a critical role in maintaining the integrity of mitochondrial complex IV. These findings provide insights into the pathogenic mechanism of REEP1 deficiency in neurodegenerative diseases and suggest a potential therapeutic target for ALS.
NEUROSCIENCE BULLETIN
(2023)
Article
Neurosciences
Ana Laura M. R. Tomiyama, Luciana Politti Cartarozzi, Liliande Oliveira Coser, Gabriela Bortolanca Chiarotto, Alexandre L. R. Oliveira
Summary: This study investigated the influence of IFN beta treatment on ALS mice. The results showed that low dosage of IFN beta increased the expression of MHC-I, leading to neuroprotection and immunomodulation during the presymptomatic phase of ALS. The treatment also affected synaptic preservation and microglia activation. Additionally, IFN beta treatment decreased the expression of the anti-inflammatory cytokine IL4.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Geriatrics & Gerontology
Tianchu Gao, Jia Huo, Cheng Xin, Jing Yang, Qi Liu, Hui Dong, Rui Li, Yaling Liu
Summary: This study found that overexpression of RabGGTB can delay the progression of the SOD1(G93A) mouse ALS model, reduce protein misfolding and glial overactivation, and have potential therapeutic effects on reducing abnormal aggregation of SOD1 in ALS.
FRONTIERS IN AGING NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Iago Mendez-Lopez, Francisco J. Sancho-Bielsa, Tobias Engel, Antonio G. Garcia, Juan Fernando Padin
Summary: Recent research on amyotrophic lateral sclerosis (ALS) suggests that non-autonomous motor neuron death hypothesis may involve other cells besides motor neurons, with mitochondria playing a crucial role in ALS pathophysiology. Mutant SOD1 protein and down-regulation of OPA1 in the sympathoadrenal system of ALS mouse model lead to mitochondrial ultrastructure alterations and functional impairments prior to clinical symptoms. These findings highlight the potential therapeutic target of OPA1 and the relevance of sympathetic impairment in ALS progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Zikai Xin, Hui Dong, Rui Li, Tongyang Niu, Xin Li, Cheng Xin, Jia Huo, Qi Liu, Yaling Liu
Summary: In SOD1(G93A) mice, pathological alterations in the structural components responsible for intestinal movement may contribute to delayed bowel movement frequently observed in SOD1(G93A) mice and in individuals with ALS who experience increased constipation. This study provides novel insights into the potential involvement of the gut-brain axis in ALS.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2023)
Article
Nutrition & Dietetics
Yoshihiro Kitaoka, Soju Seki, Sou Kawata, Akira Nishiura, Kohei Kawamura, Shin-ichiro Hiraoka, Mikihiko Kogo, Susumu Tanaka
Summary: ALS patients commonly experience feeding disorders, and this study found that mastication movements are altered and associated with body weight loss in an ALS mouse model. The study also identified a decrease in the activity of specific neurons related to feeding behavior.
Article
Biochemistry & Molecular Biology
Julia Post, Anja Schaffrath, Ian Gering, Sonja Hartwig, Stefan Lehr, N. Jon Shah, Karl-Josef Langen, Dieter Willbold, Janine Kutzsche, Antje Willuweit
Summary: The study demonstrates that RD2RD2 has therapeutic potential in SOD1(G93A) mice, significantly improving performance in behavioral and motor coordination tests and slowing the progression of neurodegenerative phenotype. Additionally, treatment of SOD1(G93A) mice leads to a significant reduction in glial cell activation and rescue of neurons.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Xiaojiao Xu, Qiu Yang, Zheyi Liu, Rong Zhang, Hang Yu, Manli Wang, Sheng Chen, Guowang Xu, Yaping Shao, Weidong Le
Summary: This study aimed to investigate the molecular changes at both metabolic and proteomic levels during the longitudinal progression of ALS and identify the most critical metabolic pathways and underlying mechanisms involved in ALS pathophysiological changes. The study found differential expressions of metabolites involved in purine metabolism, methionine cycle, and glycolysis in ALS mice, and abnormal expressions of enzymes in these metabolic pathways were also confirmed. Additionally, fatty acid metabolism, TCA cycle, arginine and proline metabolism, and folate-mediated one-carbon metabolism were significantly altered in this disease. The identified differential metabolites and proteins could complement existing data on metabolic reprogramming in ALS and provide new insights into the pathological mechanisms and therapeutic targets of ALS.
NEUROBIOLOGY OF DISEASE
(2023)
Article
Neurosciences
Xiaojiao Xu, Jingjing Zhang, Song Li, Murad Al-Nusaif, Qinming Zhou, Sheng Chen, Weidong Le
Summary: The study found that neuroinflammation in the early pathogenesis of ALS is associated with increased immune-related genes, and BST2 may serve as a potential target for ameliorating microglia-mediated neuroinflammation pathologies in ALS.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Medicine, General & Internal
Olga Kolesova, Flaminia Tomassetti, Paola Cerini, Davide Finucci, Giordano Turchetti, Francesca Capogreco, Sergio Bernardini, Graziella Calugi, Massimo Pieri
Summary: The Elecsys SARS-CoV-2 antigen test showed an overall sensitivity of 85% and specificity of 100%, with a higher sensitivity of 94% for samples with Ct values < 28. The agreement between the antigen test and RT-qPCR was 89.1% for total data and 95.9% for samples with Ct < 28. The antigen test performed well in detecting high viral loads but had lower sensitivity with lower viral loads.
IRISH JOURNAL OF MEDICAL SCIENCE
(2022)
Article
Virology
Massimo Pieri, Eleonora Nicolai, Marzia Nuccetelli, Serena Sarubbi, Flaminia Tomassetti, Martina Pelagalli, Marilena Minieri, Alessandro Terrinoni, Sergio Bernardini
Summary: This study evaluated the performance of the RapiRead™ reader and TestNOW™ COVID-19 NAb assay compared to chemiluminescence immunoassay (CLIA) methods. The results showed good correlation between the rapid lateral flow immunoassay (LFIA) test system and CLIA methods. The RapiRead™ reader and TestNOW™ COVID-19 NAb assay are quantitative, rapid, and valid methods for detecting SARS-CoV-2 neutralizing antibodies, making them useful tools for screening studies and vaccine efficacy assessment in non-laboratory settings.
ARCHIVES OF VIROLOGY
(2022)
Article
Nutrition & Dietetics
Sabrina Caporali, Alessandro De Stefano, Cinzia Calabrese, Alfredo Giovannelli, Massimo Pieri, Isabella Savini, Manfredi Tesauro, Sergio Bernardini, Marilena Minieri, Alessandro Terrinoni
Summary: Flavonoids are interesting molecules synthesized by plants, with important nutritional and anti-inflammatory properties in the human body. The biochemical properties of flavonoid molecules provide new perspectives for long-term health in healthy individuals, as well as their use as adjunctive treatment for specific diseases.
Article
Clinical Neurology
Tommaso Schirinzi, Henri Zenuni, Piergiorgio Grillo, Roberta Bovenzi, Gisella Guerrera, Francesca Gargano, Massimo Pieri, Sergio Bernardini, Nicola Biagio Mercuri, Luca Battistini, Giulia Maria Sancesario
Summary: This study found that there were no quantitative differences in serum biomarker levels between Parkinson's disease patients and control subjects. However, only Parkinson's disease patients showed associations between levels of tau and amyloid-beta-42 in inter-fluids (serum-CSF). In addition, the serum content of tau protein was inversely correlated with cognitive performance.
FRONTIERS IN NEUROLOGY
(2022)
Article
Medical Laboratory Technology
Silvia Angeletti, Jacopo M. Legramante, Maria Stella Lia, Loreta D'Amico, Marta Fogolari, Eleonora Cella, Marina De Cesaris, Fabio De Angelis, Massimo Pieri, Alessandro Terrinoni, Sergio Bernardini, Marilena Minieri
Summary: Midregional proadrenomedullin (MR-proADM) has been demonstrated to be a helpful biomarker for evaluating disease severity in patients with sepsis and pneumonia. This study evaluated the stability of MR-proADM in different biological matrices and found that serum should not be used as a sample matrix while heparin plasma can be used without reservation in clinical routine and emergency samples.
LABORATORY MEDICINE
(2023)
Article
Medicine, General & Internal
Marilena Minieri, Vito N. Di Lecce, Maria Stella Lia, Massimo Maurici, Francesca Leonardis, Susanna Longo, Luca Colangeli, Carla Paganelli, Stefania Levantesi, Alessandro Terrinoni, Vincenzo Malagnino, Domenico J. Brunetti, Alfredo Giovannelli, Massimo Pieri, Marco Ciotti, Cartesio D'Agostini, Mariachiara Gabriele, Sergio Bernardini, Jacopo M. Legramante
Summary: This study analyzed data from 321 COVID-19 patients to assess the potential role of the biomarker MR-proADM in predicting in-hospital mortality and the need for ventilation. The results showed that MR-proADM had a good predictive performance in these aspects.
Article
Medicine, General & Internal
Marco Infante, Massimo Pieri, Santina Lupisella, Ali Mohamad, Sergio Bernardini, David Della-Morte, Andrea Fabbri, Alberto De Stefano, Marco Iannetta, Lorenzo Ansaldo, Angela Crea, Massimo Andreoni, Maria Morello
Summary: Type 2 diabetes mellitus and impaired kidney function are linked to a higher risk of adverse outcomes in COVID-19. This study found that admission estimated glomerular filtration rate and white blood cell count were significantly associated with in-hospital mortality among patients with diabetes and COVID-19.
CURRENT MEDICAL RESEARCH AND OPINION
(2023)
Article
Infectious Diseases
Paola Cerini, Francesca Rita Meduri, Flaminia Tomassetti, Isabella Polidori, Marta Brugneti, Eleonora Nicolai, Sergio Bernardini, Massimo Pieri, Francesco Broccolo
Summary: The World Health Organization has identified three categories of pathogens based on the need for new antibiotics: critical, high, and medium priority. The prevalence of antibiotic resistant bacteria, such as CPO, MRSA, and VRE, has increased from 2019 to 2022 in both nosocomial and community patients. This highlights the importance of implementing preventive and control measures to reduce the spread of multidrug-resistant pathogens.
Article
Biochemistry & Molecular Biology
Alessandro Terrinoni, Gabriele Sala, Ernesto Bruno, Consuelo Pitolli, Marilena Minieri, Massimo Pieri, Alessandra Gambacurta, Elena Campione, Riccardo Belardi, Sergio Bernardini
Summary: Ichthyoses are genetically determined cornification disorders of the epidermis. Phenotypic overlap among different genetic entities can occur due to variable penetrance of mutations. This study identified a family with mild phenotypes resembling erythrokeratodermia variabilis (EKV), but genetic analysis revealed double heterozygosity for two different mutations in the ABCA12 gene responsible for harlequin ichthyosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Public, Environmental & Occupational Health
Salma Younes, Eleonora Nicolai, Duaa W. Al-Sadeq, Nadin Younes, Nader Al-Dewik, Haissam Abou-Saleh, Bushra Y. Abo-Halawa, Ali Hussein Eid, Massimo Pieri, Na Liu, Hanin I. Daas, Hadi M. Yassine, Parveen B. Nizamuddin, Laith J. Abu-Raddad, Gheyath K. Nasrallah
Summary: This study compared antibody responses between vaccinated individuals and naturally infected individuals over a 10-month period. The vaccinated individuals showed significantly higher antibody responses in the first 6 months compared to the naturally infected individuals. However, antibody responses started to decline after the 7th month in the vaccinated group. These findings have important implications for the development of long-lasting protection strategies.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2023)
Review
Biochemistry & Molecular Biology
Michele Basilicata, Massimo Pieri, Giulia Marrone, Eleonora Nicolai, Manuela Di Lauro, Vincenza Paolino, Flaminia Tomassetti, Ilaria Vivarini, Patrizio Bollero, Sergio Bernardini, Annalisa Noce
Summary: Saliva is a complex fluid that plays a crucial role in various physiological processes and functions, including oral health, taste, digestion, and immunological defenses. The composition of saliva and the oral microbiome can be influenced by factors such as diet and smoking habits, and changes in these factors can lead to the development of systemic illnesses. This review explores the potential of saliva as a new tool for early detection of conditions such as oral diseases and chronic non-communicable diseases, including chronic kidney disease. The correlation between oral and systemic diseases as well as oral and gut microbiota dysbiosis is also examined. Salivary parameters could serve as a new device for detecting oral and systemic pathologies, and modulating oral and gut microbiota through prebiotics, postbiotics, or symbiotics may offer a new approach for managing oral diseases and chronic kidney disease.
Article
Medicine, General & Internal
Cartesio D'Agostini, Jacopo M. Legramante, Marilena Minieri, Vito N. Di Lecce, Maria Stella Lia, Massimo Maurici, Ilaria Simonelli, Marco Ciotti, Carla Paganelli, Alessandro Terrinoni, Alfredo Giovannelli, Massimo Pieri, Mariacarla Gallu, Vito Dell'Olio, Carla Prezioso, Dolores Limongi, Sergio Bernardini, Antonio Orlacchio, Aw Tar-Choon
Summary: The study aimed to assess the association between CT scan results and MR-proADM levels in order to improve the prediction of in-hospital mortality of COVID-19 patients at the triage. The key role of CT score and MR-proADM levels in determining the correct clinical setting for these patients was also evaluated. The results showed that using CT score in combination with MR-proADM levels was particularly effective in risk stratification and decision-making in the emergency department, optimizing hospital resources.
Article
Biochemistry & Molecular Biology
Eleonora Nicolai, Flaminia Tomassetti, Martina Pelagalli, Serena Sarubbi, Marilena Minieri, Alberto Nisini, Marzia Nuccetelli, Marco Ciotti, Massimo Pieri, Sergio Bernardini
Summary: This study evaluated the level of neutralizing antibodies in individuals who received a three-vaccination series and found that after six months, the antibody titer significantly increased. Participants who received three vaccine doses had an antibody level of over 1000 BAU/mL five months after the third dose, indicating a low risk of infection.
Review
Biology
Maria Morello, Dominga Amoroso, Felicia Losacco, Marco Viscovo, Massimo Pieri, Sergio Bernardini, Gaspare Adorno
Summary: A urine test can be used as a non-invasive method for early monitoring of potential kidney damage. This review discusses the utility of urinalysis in predicting the severity of COVID-19, based on 68 articles obtained from PubMed studies. The review highlights the relationship between urinary markers, such as haematuria and proteinuria, and the severity of the disease, as well as the typical alterations of the urinary sediment in COVID-19-associated AKI patients.
Article
Medicine, Research & Experimental
Eleonora Nicolai, Serena Sarubbi, Martina Pelagalli, Valerio Basile, Alessandro Terrinoni, Marilena Minieri, Oreste Cennamo, Sandro Grelli, Sergio Bernardini, Massimo Pieri
Summary: Infectious diseases like HIV and HBV have a global impact on public health and healthcare costs. Timely diagnosis plays a central role in controlling the spread of these infections. This study compared the performance of the Abbott system and the new analyser Mindray 1200i in detecting HBV and HIV infections. Clinical serum samples from randomly selected patients were tested and compared with the routine instrument, Abbott Architect. The results showed that the Mindray CL-1200i platform offers high-level performance with accurate and consistent test results, making it a valuable tool for routine analysis.