期刊
CELLULAR AND MOLECULAR NEUROBIOLOGY
卷 29, 期 1, 页码 87-95出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10571-008-9300-3
关键词
Brain microvascular endothelial cells; Hemolysate; Inflammation; Intercellular adhesion molecule-1; Monocyte chemoattractant protein-1; NF-kappa B; Reactive oxygen species
资金
- National Natural Science Foundation of China [30700866]
In order to determine the possible effects of hemolysate on brain microvascular endothelial cells (BMECs), we examined the effects of hemolysate on the expression of intercellular adhesion molecule-1 (ICAM-1) and monocyte chemoattractant protein-1 (MCP-1), generation of reactive oxygen species (ROS), and NF-kappa B activation in rat BMECs. Hemolysate induced the expression of ICAM-1 and MCP-1 in endothelial cells. In addition, hemolysate stimulated nuclear translocation of the p65 subunit of NF-kappa B, and NF-kappa B DNA-binding activity in BMECs. Furthermore, hemolysate increased ROS generation, and hemolysate-induced ICAM-1 and MCP-1 expression and NF-kappa B activation were abrogated in the presence of the direct scavenger of ROS. Taken together, our results indicate that hemolysate can induce inflammatory responses that increase expression of ICAM-1 and MCP-1, through ROS-dependent NF-kappa B activation in BMECs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据