期刊
CELLULAR AND MOLECULAR LIFE SCIENCES
卷 70, 期 17, 页码 3145-3156出版社
SPRINGER BASEL AG
DOI: 10.1007/s00018-012-1222-z
关键词
Deamination; Mutagenesis; Endonuclease; Inosine; Xanthosine; Uridine; Oxanosine; DNA repair
资金
- CSREES/USDA [SC-1700274, 6059]
- Department of Defense [W81XWH-10-1-0385]
- National Institutes of Health [GM090141]
Endonuclease V (endo V) was first discovered as the fifth endonuclease in Escherichia coli in 1977 and later rediscovered as a deoxyinosine 3' endonuclease. Decades of biochemical and genetic investigations have accumulated rich information on its role as a DNA repair enzyme for the removal of deaminated bases. Structural and biochemical analyses have offered invaluable insights on its recognition capacity, catalytic mechanism, and multitude of enzymatic activities. The roles of endo V in genome maintenance have been validated in both prokaryotic and eukaryotic organisms. The ubiquitous nature of endo V in the three domains of life: Bacteria, Archaea, and Eukaryotes, indicates its existence in the early evolutionary stage of cellular life. The application of endo V in mutation detection and DNA manipulation underscores its value beyond cellular DNA repair. This review is intended to provide a comprehensive account of the historic aspects, biochemical, structural biological, genetic and biotechnological studies of this unusual DNA repair enzyme.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据