Article
Entomology
Mulood Mohammadi Bavani, Seyyed Jaavad Seyyedzadeh, Ehsan Radi, Azam Malekian, Mahdiyeh Hoseinpour, Saber Gholizadeh
Summary: Maggot debridement therapy is a method that utilizes sterile fly larvae for treating wounds and ulcers. The study found that using hydrogen peroxide solutions can effectively eliminate bacteria in the eggs and improve the sterility of the larvae.
JOURNAL OF MEDICAL ENTOMOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Rebecca J. Davis, Esther J. Belikoff, Allison N. Dickey, Elizabeth H. Scholl, Joshua B. Benoit, Maxwell J. Scott
Summary: The common green bottle blow fly Lucilia sericata is used for maggot debridement therapy, pest control, and forensic studies. A genome assembly of 565.3 Mb with 14,704 protein coding genes and 1709 non-coding genes was reported, with highly expressed genes identified in different tissues under various conditions. The genomic resources will support future genetic studies and the development of genetically engineered strains for L. sericata control and biotechnology-enhanced maggot therapy.
Article
Multidisciplinary Sciences
Takuma Yoshida, Hiroka Aonuma, Saori Otsuka, Hidetoshi Ichimura, Erisha Saiki, Kosei Hashimoto, Manabu Ote, Sari Matsumoto, Kimiharu Iwadate, Takeshi Miyawaki, Hirotaka Kanuka
Summary: Maggot debridement therapy (MDT) is a therapeutic wound treatment method that uses live fly larvae to remove necrotic tissues and promote wound healing. The study developed a feeding assay to evaluate the effectiveness of different Lucilia sericata strains. It was found that a strain derived from corpses showed higher feeding activity and potential improvement for MDT.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Meriem Taleb
Summary: This study compared three antiseptic solutions for disinfecting Lucilia sericata eggs, finding that 0.5% NaOCl was the best method in terms of eliminating bacterial contamination. Sterility testing of medicinal maggots after disinfection is crucial for preventing secondary infections.
WOUND REPAIR AND REGENERATION
(2021)
Review
Entomology
U. Gazi, A. Taylan-Ozkan, K. Y. Mumcuoglu
Summary: Chronic wounds are a major public health concern due to changes in lifestyle and aging population. Treatment strategies vary depending on the type of wound, with maggot therapy being a biological debridement method that can influence all phases of wound repair.
MEDICAL AND VETERINARY ENTOMOLOGY
(2021)
Article
Cell Biology
Fatma Kubra Tombulturk, Gonul Kanigur-Sultuybek
Summary: Chronic wounds caused by underlying physiological reasons can be effectively treated with maggot debridement therapy, which utilizes enzymes in maggots to promote wound healing and has various beneficial effects such as antibacterial, anti-inflammatory, angiogenic, and proliferative properties. Further research on the molecular content of maggot secretions and their biological effects is essential for understanding the mechanism underlying this therapy and its potential as a therapeutic agent in wound healing.
WOUND REPAIR AND REGENERATION
(2021)
Article
Biology
Mustafa Becerikli, Christoph Wallner, Mehran Dadras, Johannes M. Wagner, Stephanie Dittfeld, Birger Jettkant, Falk Gestmann, Heinz Mehlhorn, Tim Mehlhorn-Diehl, Marcus Lehnhardt, Bjoern Behr
Summary: Larval extract from Lucilia sericata can reduce the survival of free-floating Pseudomonas aeruginosa and destroy the biofilm stability of both P. aeruginosa and Staphylococcus aureus. SEM analyses showed crack and gap formations on P. aeruginosa biofilm surface, and decreased expression of P. aeruginosa biofilm maturation and virulence genes after treatment with larval extract.
Article
Entomology
German Alberto Tellez Ramirez, Juan Felipe Osorio-Mendez, Diana Carolina Henao Arias, Lily Johanna S. Toro, Juliana Franco Castrillon, Maribel Rojas-Montoya, Jhon Carlos Castano Osorio
Summary: The study identified 155 novel HDP sequences from nine transcriptomes of seven species of Coleoptera, enriching the diversity of antimicrobial peptides within this insect family. These sequences were identified based on their similarity to known HDP insect families, including defensins, cecropins, attancins, and coleoptericins.
JOURNAL OF INSECT SCIENCE
(2021)
Article
Veterinary Sciences
Ugur Uslu, Onur Ceylan, Abdullah Kucukyaglioglu, Huseyin Koray Akdeniz
Summary: This case study confirmed the effectiveness of using maggot debridement therapy on a chronic infected wound, showing the gradual healing process from a large and infected wound to a smaller healed wound.
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
(2021)
Article
Multidisciplinary Sciences
Dariusz Bazalinski, Pawel Wiech, Paulina Szymanska, Marek Muster, Maria Kozka
Summary: The use of biological therapy with Lucilia sericata larvae has supporters worldwide, but its application is limited due to the lack of awareness and experience among medical personnel. However, case reports suggest that maggot therapy can be supported by trained medical staff and caregivers. Maggot Debridement Therapy is considered a fast and effective method for wound preparation, which is safe and acceptable for patients and caregivers in outpatient settings.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Entomology
Julie Pinto, Paola A. Magni, R. Christopher O'Brien, Ian R. Dadour
Summary: This study compared the wingbeat frequency of four species of calyptrate flies and found differences between species and sexes. The findings suggest that wingbeat frequency could be used as a novel methodology to identify fly specimens and provide insight into fly communication.
Article
Veterinary Sciences
Ugur Uslu, Ayse Evci, Hueseyin Koray Akdeniz, Onur Ceylan
Summary: Maggot debridement therapy (MDT), although losing its importance with the advent of antibiotics, has seen an increase in recent years for treating non-healing wounds. This case report investigates the efficacy of MDT on a post-operative infected wound in a 5-month-old Doberman dog. After multiple applications of MDT, the wound showed gradual healing and positive response.
ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
(2023)
Review
Infectious Diseases
Jiahui Wu, Xia Zhou, Qiaoqiao Chen, Zhiqiang Chen, Jinyu Zhang, Lele Yang, Yuxuan Sun, Guohui Wang, Jianfeng Dai, Tingting Feng
Summary: This review provides an overview of tick defensins, including their structural classification and antimicrobial function. Tick defensins exhibit broad-spectrum antimicrobial activities against various pathogens and are considered novel candidates or targets for controlling infectious diseases.
INFECTIOUS DISEASES OF POVERTY
(2022)
Article
Entomology
Paul Brett, Kevin Lawrence, Paul Kenyon, Kristene Gedye, William Pomroy
Summary: Flystrike in New Zealand is caused by two Dipteran species, Lucilia cuprina and Lucilia sericata. This study compared the effectiveness of the LuciTrap and the Western Australian Trap, finding that both traps were effective in catching L. cuprina and L. sericata. New Zealand farmers can use either of these traps to monitor the populations of these target species.
Article
Public, Environmental & Occupational Health
Joanna Przybek Mita, Dariusz Bazalinski, Rafal Sztembis, Izabela Kuberka, Pawel Wiech
Summary: Stress is recognized as a key problem in neurological studies, with prolonged stress being detrimental to health. This study assessed the intensity of perceived stress in nurses dealing with wound care. The results showed that there were differences in stress levels among age groups, but they were not statistically significant. The highest differences in stress levels were observed between those who completed treatment courses and those who did not. The higher the stress level, the lower the willingness to undertake such therapy.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Endocrinology & Metabolism
Michal Dubsky, Alexandra Jirkovska, Libuse Pagacova, Robert Bem, Andrea Nemcova, Vladimira Fejfarova, Veronika Woskova, Edward B. Jude
JOURNAL OF DIABETES RESEARCH
(2015)
Article
Endocrinology & Metabolism
Vladimira Fejfarova, Alexandra Jirkovska, Michal Dubsky, Frances Game, Jana Vydlakova, Alena Sekerkova, Jana Franekova, Monika Kucerova, Ilja Striz, Vladimir Petkov, Robert Bem, Veronika Woskova, Andrea Nemcova, Jelena Skibova
JOURNAL OF DIABETES RESEARCH
(2016)
Article
Cell & Tissue Engineering
Andrea Nemcova, Alexandra Jirkovska, Michal Dubsky, Libor Kolesar, Robert Bem, Vladimira Fejfarova, Anna Pysna, Veronika Woskova, Jelena Skibova, Edward B. Jude
CELL TRANSPLANTATION
(2018)
Article
Cell & Tissue Engineering
Michal Dubsky, Alexandra Jirkovska, Robert Bem, Vladimira Fejfarova, Martin Varga, Libor Kolesar, Libuse Pagacova, Eva Sykova, Edward. B. Jude
CELL TRANSPLANTATION
(2014)
Review
Cell & Tissue Engineering
Anna Pysna, Robert Bem, Andrea Nemcova, Vladimira Fejfarova, Alexandra Jirkovska, Jitka Hazdrova, Edward B. Jude, Michal Dubsky
STEM CELL REVIEWS AND REPORTS
(2019)
Article
Endocrinology & Metabolism
M. Dubsky, A. Jirkovska, R. Bem, A. Nemcova, V. Fejfarova, J. Hazdrova, K. Sutoris, J. Chlupac, J. Skibova, E. B. Jude
Article
Dermatology
Vladimira Fejfarova, Hana Tibenska, Jitka Niklova, Robert Bem, Michal Dubsky, Veronika Woskova, Andrea Nemcova, Alexandra Jirkovska, Edward Jude, Vera Lanska
INTERNATIONAL JOURNAL OF LOWER EXTREMITY WOUNDS
(2019)
Article
Physiology
M. Dubsky, P. Sedivy, A. Nemcova, M. Drobny, J. Hazdrova, V. Fejfarova, R. Bem, A. Jirkovska, M. Dezortova, M. Hajek
PHYSIOLOGICAL RESEARCH
(2019)
Article
Endocrinology & Metabolism
Vladimira Fejfarova, Jaroslav Pavlu, Robert Bem, Veronika Woskova, Michal Dubsky, Andrea Nemcova, Alexandra Jirkovska, Bedrich Sixta, Karol Sutoris, Filip Thieme, David G. Armstrong, Eliska Vratna, Jitka Hazdrova, Vera Lanska
JOURNAL OF DIABETES RESEARCH
(2019)
Article
Peripheral Vascular Disease
Michal Dubsky, Vladimira Fejfarova, Robert Bem, Alexandra Jirkovska, Andrea Nemcova, Karol Sutoris, Jitka Husakova, Jelena Skibova, Edward B. Jude
Summary: The study evaluated the factors associated with nonresponse to ACT in diabetic patients with NO-CLI, revealing that inherited thrombotic disorders, particularly Leiden mutation and MTHFR 677 mutation, were significant predictors of impaired response to the treatment.
Article
Endocrinology & Metabolism
J. Husakova, R. Bem, V Fejfarova, A. Jirkovska, V Woskova, R. Jarosikova, V Lovasova, E. B. Jude, M. Dubsky
Summary: This study found that patients with CLTI and DFU who have MTHFR gene mutations, smoke, and have higher baseline CRP levels have an increased risk for major amputation after ACT. Additionally, lower transcutaneous oxygen pressure levels at 1 and 3 months after ACT may also have predictive value.
JOURNAL OF DIABETES RESEARCH
(2022)
Article
Physiology
A. Nemcova, A. Jirkovska, M. Dubsky, R. Bem, V Fejfarova, V Woskova, A. Pysna, M. Buncova
PHYSIOLOGICAL RESEARCH
(2018)
Review
Endocrinology & Metabolism
Michal Dubsky, Alexandra Jirkovska, Robert Bem, Andrea Nemcova, Vladimira Fejfarova, Edward B. Jude
DIABETES RESEARCH AND CLINICAL PRACTICE
(2017)
Letter
Peripheral Vascular Disease
Vladimira Fejfarova, Karel Roztocil, Alexandra Svedinkova, Helena Cermakova, Michal Dubsky, Robert Bem, Alexandra Jirkovska, Veronika Woskova, Andrea Nemcova, Jelena Skibova
INTERNATIONAL ANGIOLOGY
(2017)
Review
Chemistry, Medicinal
Vaclav Cerovsky, Robert Bem