Article
Biochemistry & Molecular Biology
Mattias Basle, Henry A. W. Padley, Floriane L. Martins, Gerlof Sebastiaan Winkler, Christof M. Jager, Anca Pordea
Summary: Artificial metalloenzymes were designed by inserting catalytically active metal complexes into biological scaffolds and engineering substrate binding, showing extended substrate scope. Kinetic studies revealed specific enzyme behaviors with different substrates, providing insights for further engineering of artificial metalloenzymes.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Marcel Hrubsa, Vaclav Tvrdy, Michal Kuty, Katerina Valentova, Vladimir Kren, Premysl Mladenka
Summary: This study focused on the chelating properties of flavonoids on zinc ions and their effects on zinc-containing enzymes. Nine out of 21 tested flavonoids were capable of chelating Zn2+, with baicalein and 3-hydroxyflavone being the most effective chelators under certain pH conditions. The chelation was confirmed by reversing Zn2+-induced enzymatic inhibition in bovine glutamate dehydrogenase.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Review
Chemistry, Inorganic & Nuclear
Ying-Wu Lin
Summary: This article discusses the progress in biodegradation of aromatic pollutants using native and artificial metalloenzymes. The structure-function relationship of metalloenzymes sheds light on potential applications in environmental protection, providing clues for future research directions in environmental science.
COORDINATION CHEMISTRY REVIEWS
(2021)
Article
Nutrition & Dietetics
Vaclav Tvrdy, Marcel Hrubsa, Eduard Jirkovsky, David Biedermann, Michal Kuty, Katerina Valentova, Vladimir Kren, Premysl Mladenka
Summary: Silymarin is known for its hepatoprotective effects, particularly against Amanita phalloides intoxication. It is hypothesized that silymarin may influence alcoholic liver damage by inhibiting zinc-containing alcohol dehydrogenase. Tests showed that 2,3-dehydroderivatives of silymarin significantly chelated zinc ions and inhibited alcohol dehydrogenase and glutamate dehydrogenase.
Article
Microbiology
Liam A. Wilson, Esmee G. Knaven, Marc T. Morris, Marcelo Monteiro Pedroso, Christopher J. Schofield, Thomas B. Brueck, Mikael Boden, David W. Waite, Philip Hugenholtz, Luke Guddat, Gerhard Schenk
Summary: The study investigates a fourth variant of metallo-beta-lactamase, SIE-1, which shows higher activity towards cephalosporins. The unusual preference of SIE-1 for cephalosporins and its presence in a widespread environmental organism suggest the potential for increased MBL-mediated beta-lactam resistance. Including SIE-1 in MBL inhibitor design studies could expand the therapeutic scope of much needed antiresistance drugs.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Review
Chemistry, Multidisciplinary
Marta Meneghello, Christophe Leger, Vincent Fourmond
Summary: Only two enzymes, CO dehydrogenase and formate dehydrogenase, are capable of directly reducing CO2, producing CO and formate respectively. These metalloenzymes are rapid, energy-efficient, and specific in product. Researchers have studied their mechanisms using protein film electrochemistry, and also exploited their catalytic performance to build biotechnological devices.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Chemistry, Inorganic & Nuclear
Ilker Avan, Ibrahim Kani, Leyla Calikusu
Summary: Homoleptic bis(dipyrrinato)zinc(II) complexes, (Dprm)2-Zn and (EDprm)2-Zn, were synthesized using two different dipyrrinato ligands. X-ray crystal structures revealed that these complexes are four-coordinate with distorted tetrahedral geometry. The catalytic activities of these complexes towards the oxidation of various alcohols were investigated, showing high catalytic activity in acetonitrile at 70 degrees C.
INORGANIC CHEMISTRY COMMUNICATIONS
(2023)
Article
Food Science & Technology
Hua Cao, Tuoyu Zhou, Haibo Tang, Pengya Feng, Gohar Ali, Pu Liu, Xiangkai Li
Summary: The genetically modified EcN has shown effectiveness in detoxifying ethanol metabolites in liver disease models, restoring liver function, inhibiting oxidative stress, lipid peroxidation, and inflammation, as well as promoting the recovery of gut microbiota homeostasis.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Chemistry, Multidisciplinary
Fan Tang, Wen-Cui Li, Lei He, Dongqi Wang, An-Hui Lu
Summary: This study reports a highly efficient zinc hydroxyapatite catalyst for the direct conversion of ethanol to aromatic alcohols. The catalyst exhibits the highest specific rate among all reported literature. The dehydrogenation and hydrogenation reactions of ethanol occur at the zinc site, and substituting zinc for calcium can lower the energy barrier for ethanol dehydrogenation. This work provides a strategy for the synthesis of high value-added aromatic alcohols using sustainable methods.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Nanoscience & Nanotechnology
Julia Alvarez-Malmagro, Ana R. Oliveira, Cristina Gutierrez-Sanchez, Beatriz Villajos, Ines A. C. Pereira, Marisela Velez, Marcos Pita, Antonio L. De Lacey
Summary: This study investigates the covalent bonding of Desulfovibrio vulgaris Hildenborough FdhAB formate dehydrogenase to gold and graphite electrodes, showing a compact enzyme layer anchored to the electrode surface for the reduction of CO2 to formic acid via direct electron transfer. The operational stability of CO2 electroreduction to formate by direct electron transfer is demonstrated with approximately 100% Faradaic yield.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Editorial Material
Chemistry, Multidisciplinary
Max Mortensen, Siris Laursen
Summary: By using intermetallic Pd-Zn, a series of well-defined multinuclear Pd-metal-Pd catalytic sites have been generated and studied, providing insights into their selectivity for the semi-hydrogenation of acetylene.
Article
Biochemistry & Molecular Biology
Ryan Feehan, Matthew Copeland, Meghan W. Franklin, Joanna S. G. Slusky
Summary: Recent advances have made it possible to generate high-quality structures for proteins without solved crystal structures using computational methods. However, functional data for proteins is still limited to experimental methods and homology mapping. In our laboratory, we have developed a method to distinguish between metalloenzyme and nonenzyme sites based on computational structures. Through upgrades to our physicochemical features and the use of machine learning to reduce training data labeling error, we have improved the accuracy and reliability of our classifier. Our improved model shows promising results in identifying catalytic metal sites and has the potential to uncover new enzymatic mechanisms and improve the design of metalloenzymes.
Article
Chemistry, Multidisciplinary
Chu Zheng, Zhe Ji, Irimpan I. Mathews, Steven G. Boxer
Summary: Improving enzyme activity based on the principle of electrostatic catalysis is demonstrated in this study. By enhancing the electric field in horse liver alcohol dehydrogenase through modifications in the active site, a significant rate acceleration is achieved. The effects of hydrogen bonding and metal coordination are described by the quantitative and predictive physical quantity of electric field, suggesting a new design paradigm for catalysts.
Article
Chemistry, Multidisciplinary
Ramona Jurgeleit, Benjamin Grimm-Lebsanft, Benedikt Maria Floeser, Melissa Teubner, Soren Buchenau, Laura Senft, Jonas Hoffmann, Maria Naumova, Christian Naether, Ivana Ivanovic-Burmazovic, Michael Ruebhausen, Felix Tuczek
Summary: The study demonstrates that low-temperature oxygenation of copper complexes results in the formation of unique tetranuclear mixed-valent peroxo compounds, which promote O-O bond homolysis and catalytic activity, showing efficient catalytic activity towards organic substrates.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Li Wang, Jia Zhang, Ming-Jie Han, Lu Zhang, Chao Chen, Aiping Huang, Ruipei Xie, Guosheng Wang, Jiangrui Zhu, Yuchuan Wang, Xiaohong Liu, Wei Zhuang, Yunliang Li, Jiangyun Wang
Summary: The genetic incorporation of a novel 2D-IR probe, N3Y, in the active site of DddK enzyme demonstrates potential application in investigating enzyme dynamics. Results indicate that oxidation of active-site iron to Fe-III and addition of denaturation reagents result in significant decrease in enzyme activity and active-site water motion confinement, highlighting the importance of tyrosine residues in enzyme function.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
