Article
Microbiology
Elise T. T. Zeiser, Scott A. A. Becka, John J. J. LiPuma, Krisztina M. M. Papp-Wallace
Summary: In this study, a new compound, ETX0462, was evaluated for its activity against multidrug-resistant Burkholderia cepacia complex and Burkholderia gladioli, with the results showing that ETX0462 demonstrated superior in vitro activity compared to currently available treatment options.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Multidisciplinary Sciences
Carmina Micelli, Yunfei Dai, Nicole Raustad, Ralph R. Isberg, Christopher G. Dowson, Adrian J. Lloyd, Edward Geisinger, Allister Crow, David I. Roper
Summary: Acinetobacter baumannii is a gram-negative bacterial pathogen that causes challenging nosocomial infections. The study reveals the molecular details of cell wall elongation inhibition upon zinc starvation in A. baumannii. The findings offer a perspective for dual inhibition of zinc-dependent peptidoglycan synthases and metallo-beta-lactamases by metal-chelating agents.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Surgery
Nathaniel B. Wilhelm, Thomas J. Bonsall, Clayton L. Miller
Summary: Reported BL allergic patients have an increased odds of developing SSI compared to NBL allergic patients. The increased risk is likely related to administration of NBL antibiotic regimens. Thorough antibiotic allergy history collection can be an important tool for preventing SSI.
Article
Microbiology
Shannon G. Murphy, Andrew N. Murtha, Ziyi Zhao, Laura Alvarez, Peter Diebold, Jung-Ho Shin, Michael S. VanNieuwenhze, Felipe Cava, Tobias Doerr
Summary: In Vibrio cholerae, endopeptidases are essential for proper cell elongation and division, while aPBPs become crucial for maintaining structural integrity during endopeptidase insufficiency, and the Rod system remains active but contributes minimally to cell expansion under these conditions.
Article
Chemistry, Medicinal
Jun Sato, Hiroki Kusano, Toshiaki Aoki, Satoru Shibuya, Katsuki Yokoo, Kazuo Komano, Takuya Oguma, Shuhei Matsumoto, Rio Nakamura, Takafumi Sato, Kenji Yamawaki
Summary: The emergence of carbapenem-resistant enterobacterales (CREs) is a significant global clinical and public health threat. This study reports the discovery of a potent tricyclic beta-lactam compound that overcomes resistance mechanisms other than beta-lactamase production. The compound showed promising therapeutic efficacy in a mouse lung infection model, making it a potential drug candidate for infectious diseases caused by CREs.
ACS INFECTIOUS DISEASES
(2022)
Review
Immunology
Dingle Yu, Danchun Guo, Yuejie Zheng, Yonghong Yang
Summary: With the widespread use of antibiotics, antimicrobial resistance (AMR) has become a global problem that endangers public health. The gene encoding penicillin binding protein 2X (pbp2x) has been found to be associated with group A Streptococcus (GAS) with reduced beta-lactam susceptibility.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Silvia Lopez-Arguello, Maria Montaner, Amanda Marmol-Salvador, Ana Velazquez-Escudero, Fernando Docobo-Perez, Antonio Oliver, Bartolome Moya
Summary: The lack of effective antibiotics for Neisseria gonorrhoeae and the worldwide spread of resistant strains are the main causes of a worsening global health crisis. Beta-lactam antibiotics have been the main treatment for gonococci, but we need more insights to design optimized therapies.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Jinglan Wang, Laura Alvarez, Silvia Bulgheresi, Felipe Cava, Tanneke den Blaauwen
Summary: Peptidoglycan is crucial for bacterial survival and cell shape maintenance. Research on the gamma proteobacterium Candidatus Thiosymbion oneisti reveals its unique PG composition with high peptide cross-linkage and short glycan chains, as well as the involvement of endopeptidase PBP4 in septum formation. PBP4 shows different beta-lactam selectivity and potential allosteric binding site, suggesting a role in PG cleavage during longitudinal cell division and adaptation to a symbiotic lifestyle.
Article
Microbiology
Si Hyoung Park, Umji Choi, Su-Hyun Ryu, Han Byeol Lee, Jin-Won Lee, Chang-Ro Lee
Summary: This study revealed the distinct roles of DacA in vancomycin and beta-lactam resistance, providing important insights into the regulation of bacterial antibiotic resistance.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Stefan Freischem, Immanuel Grimm, Arancha Lopez-Perez, Dieter Willbold, Burkhard Klenke, Cuong Vuong, Andrew J. Dingley, Oliver H. Weiergraber
Summary: Novel antimicrobial strategies are urgently needed to combat the rising threat of multi drug resistant bacterial strains. Penicillin binding proteins are of particular interest due to their good accessibility and reduced risk of side effects on humans. The newly developed beta-lactam antibiotic demonstrates broad antimicrobial activity and improved resistance to beta-lactamases.
Article
Biochemistry & Molecular Biology
Piotr Henryk Malecki, Pawel Mitkowski, Elzbieta Jagielska, Karolina Trochimiak, Stephane Mesnage, Izabela Sabala
Summary: The M23 family members, such as lysostaphin or LytM, are well-characterized glycyl-glycine hydrolases. Enzymes like EnpA(CD) from Enterococcus faecalis exhibit a broad specificity for cleaving D,L peptide bonds. The substrate-binding groove architecture and specificity of M23 enzymes suggest that they prefer peptides with short side chains or alternating chirality of amino acids.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jordan Delisle, Baptiste Cordier, Stephane Audebert, Matthieu Pophillat, Caroline Cluzel, Leon Espinosa, Christophe Grangeasse, Anne Galinier, Thierry Doan
Summary: Research indicates that TseB may be a novel regulator of PBPs, interacting with PBP2A to play a crucial role in cell wall elongation and spore germination.
MOLECULAR MICROBIOLOGY
(2021)
Article
Microbiology
Natalia Kurepina, Liang Chen, Kaelea Composto, Dalin Rifat, Eric L. Nuermberger, Barry N. Kreiswirth
Summary: In this study, we used CRISPRi assay to control the gene expressions of pbpB and cwIM in Mycobacterium abscessus and evaluated their contribution to beta-lactam susceptibility. Our results demonstrated that CRISPRi can be used as an experimental approach to study drug/target interactions in M. abscessus.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Joan Marunga, Eunhye Goo, Yongsung Kang, Ingyu Hwang
Summary: Bacteria have specific signaling systems to overcome selective pressure, such as exposure to antibiotics. The two-component system (TCS) plays an important role in the development of antibiotic resistance. The findings provide a new perspective on antibiotic resistance mechanisms and suggest a different therapeutic approach for successful control of bacterial pathogens.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Polymer Science
Cansu Ilke Kuru, Fulden Ulucan-Karnak, Sinan Akgol
Summary: We developed a selective and low-cost metal-chelated nanoparticle system for the removal of the Pen G antibiotic. The system consisted of poly(glycidyl methacrylate) nanoparticles covalently bound with iminodiacetic acid and modified with Cu2+ to target Pen G specifically. The optimized system showed a high adsorption capacity for Pen G and maintained its performance over multiple cycles.