期刊
CELLULAR AND MOLECULAR LIFE SCIENCES
卷 65, 期 16, 页码 2613-2620出版社
SPRINGER BASEL AG
DOI: 10.1007/s00018-008-8164-5
关键词
oligoadenylate synthetase; archaeal CCA-adding enzyme; poly adenosine polymerase; interferon; RNA metabolism
资金
- Senior Researcher Fellowship
- Novo Nordisk Foundation
- Novo Nordisk
- Danish Medical Research Council [22-04-0704]
The 2'-5' oligoadenylate synthetases (OAS) are interferon-induced antiviral enzymes that recognise virally produced dsRNA and initiate an RNA destabilisation within the infected cell. We compared the structure of OAS to that of poly adenosine polymerase (PAP) and the class I CCA-adding enzyme from Archeoglobus fulgidus (AfCCA). This comparison revealed a strong structural homology between the three enzyme families. In particular, the active sites of OAS and CCA class I enzymes are highly conserved. We conducted an extensive mutagenesis of amino acid residues within the putative active site in OAS, thereby identifying enzymatically important residues and confirming the common active site architecture for OAS and the AfCCA. Our findings also have profound implications for our understanding of the evolutionary origin of the OAS enzymes and suggest that the OAS proteins diverged from a common 3'-specific ancestor at the beginning of metazoan evolution.
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