Article
Genetics & Heredity
Lucija Zenic, Denis Polancec, Damir Hudetz, Zeljko Jelec, Eduard Rod, Dinko Vidovic, Mario Staresinic, Srecko Sabalic, Trpimir Vrdoljak, Tadija Petrovic, Fabijan Cukelj, Vilim Molnar, Martin Cemerin, Vid Matisic, Petar Brlek, Zrinka Djukic Koroljevic, Igor Boric, Gordan Lauc, Dragan Primorac
Summary: The therapy using mesenchymal stem/stromal cells (MSC) shows promise in treating knee osteoarthritis, but lacks standardized protocols. By comparing stromal vascular fractions from adipose tissue, differences in MSC marker expression and progenitor cell ratios between female and male patients were observed, indicating a differential phenotype pattern even after microfragmentation. This study provides insights into the heterogeneity of adipose MSC subpopulations for osteoarthritis treatment, emphasizing interesting differences between women and men.
Article
Agriculture, Dairy & Animal Science
Gabriele Scattini, Martina Pellegrini, Giulio Severi, Monica Cagiola, Luisa Pascucci
Summary: Adipose-derived MSCs are commonly isolated by collagenase digestion, but waste tissue fragments contain cells that adhere belatedly to the plastic. In this study, three sets of cells that adhered at different time points were analyzed for their properties, and no significant differences were found between the three subpopulations. This procedure improves MSCs yield and shortens the time required for clinical applications.
Review
Biochemistry & Molecular Biology
Ibrahim Vargel, Ali Tuncel, Nilsu Baysal, Irem Hartuc-Cevik, Feza Korkusuz
Summary: SVF has the potential to modulate inflammation and initiate regeneration in joint tissues, making it a potential approach for treating knee osteoarthritis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
S. Gandolfi, B. Pileyre, L. Drouot, I. Dubus, I. Auquit-Auckbur, J. Martinet
Summary: Muscle regeneration is a complex process that requires precise interaction between immune and muscular cells. The mechanisms underlying autoimmune myopathies are not fully understood, but chronic inflammation and decreased muscle regenerative capacity play a role. Immunosuppressive treatments can be ineffective, and alternative therapies such as stromal vascular fraction show promise but lack a clear understanding of their active components and mechanisms of action.
CELL DEATH DISCOVERY
(2023)
Article
Surgery
Kevin M. Urlaub, Kavitha Ranganathan, Jeremy V. Lynn, Alexandra O. Luby, Lauren N. Patrick, Noah S. Nelson, Alexis Donneys, Steven R. Buchman
Summary: Stromal vascular fraction therapy has shown significant efficacy in enhancing bone healing in irradiated fracture repair, improving union rates and bone vascularization metrics while restoring bone cellularity and maturity despite preoperative irradiation. This study provides a promising foundation for the development of noncultured, cell-based therapies for clinical implementation.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2021)
Article
Biotechnology & Applied Microbiology
Paul Girard, Joelle Dulong, Jerome Duisit, Camille Mocquard, Simon Le Gallou, Benoit Chaput, Elise Lupon, Eric Watier, Audrey Varin, Karin Tarte, Nicolas Bertheuil
Summary: This study optimized the nanofat grafting technique to increase the yield of adipose-derived stem cells (ASCs) extraction. The results showed that the stromal vascular fraction (SVF) obtained by 20 inter-syringe passages contained more cells, including ASCs, and had better anti-inflammatory properties. This study provides an important foundation for decellularized tissue methods that can provide the required quantity and quality of cells in a clinical setting.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Medicine, General & Internal
Robert Ossendorff, Alessandra Menon, Frank A. Schildberg, Pietro S. Randelli, Sebastian Scheidt, Christof Burger, Dieter C. Wirtz, Davide Cucchi
Summary: The global distribution and quality of studies using adipose tissue derivates in orthopedics were assessed through a systematic review. Among the 82 included studies, 70% focused on knee disorders, mainly osteoarthritis. Approximately 26% of the studies investigated expanded adipose-derived stem/stromal cells (ADSCs), with 72% of these using stromal vascular fraction (SVF). The overall quality of the studies varied, with a higher score found in studies involving expanded ADSCs compared to SVF.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Mauro Vasella, Kevin Arnke, Dalia Dranseikiene, Elia Guzzi, Francesca Melega, Gregory Reid, Holger Jan Klein, Riccardo Schweizer, Mark W. Tibbitt, Bong-Sung Kim
Summary: In this study, the feasibility of using methacrylated gelatin (GelMA) as a scaffold for mechanically processed stromal vascular fraction (mSVF) was investigated. The results showed that GelMA maintained cell viability and proliferation, and released growth factors, which had a promoting effect on both mSVF and dermal fibroblasts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Oncology
Rawan Al-kharboosh, Jonathan Jude Perera, Alexandra Bechtle, Guojun Bu, Alfredo Quinones-Hinojosa
Summary: Neurodegenerative disorders are characterized by the irreversible loss of cognitive functions and CNS structures, and the lack of preventive and regenerative measures has led to traditional symptomatic management as the only available therapeutic recourse. However, recent research suggests that the adipose-derived stromal vascular fraction (SVF) may hold promise as a cellular treatment for neurodegenerative diseases, with improvements seen in clinical applications for conditions such as Multiple Sclerosis, Alzheimer's disease, and Parkinson's disease.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Agriculture, Dairy & Animal Science
Hanan Hendawy, Akiko Uemura, Danfu Ma, Ryosuke Namiki, Haney Samir, Mahmoud F. Ahmed, Ahmed Elfadadny, Hussein M. El-Husseiny, Cheng Chieh-Jen, Ryou Tanaka
Summary: The study addressed the ideal adipose tissue harvesting site for obtaining high-quality stromal vascular fraction (SVF) cells in middle-aged to old dogs. The peri-ovarian region was found to yield the highest viability and cellular yield of SVF cells with greater expression of adipose-derived mesenchymal stem cell markers, making it the preferred AT sampling site for canine regenerative therapies.
Article
Pharmacology & Pharmacy
Bong-Sung Kim, Shih-Heng Chen, Mauro Vasella, Marco Guidi, Epameinondas Gousopoulos, Nicole Lindenblatt, Huang-Kai Kao
Summary: This study investigates the in vivo fate of mechanically processed stromal vascular fraction (mSVF) for regenerative purposes. By establishing an arteriovenous (AV) shunt model in rats, the study shows that vascularized mSVF can proliferate and express adipocyte-specific markers.
Article
Cell & Tissue Engineering
Fermin Robledo, Lila Gonzalez-Hodar, Pablo Tapia, Ana-Maria Figueroa, Fernando Ezquer, Victor Cortes
Summary: Researchers successfully extracted multipotent cells from the brown adipose tissue of newborn mice, forming adipose spheroids with complex organoid structure and enhanced leptin secretion upon insulin stimulation. These findings have important implications for the potential therapeutic application of adipose tissue-derived stromal vascular fraction cells.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Stefan Wolf, Jeremy W. Deuel, Maija Hollmen, Gunther Felmerer, Bong-Sung Kim, Mauro Vasella, Lisanne Gruenherz, Pietro Giovanoli, Nicole Lindenblatt, Epameinondas Gousopoulos
Summary: Lipedema is characterized by disproportionate adipose tissue increase in the limbs, with a distinct systemic cytokine profile and metabolic activity compared to healthy adipose tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Yuan Cai, Fang Zhang, Jingwei Feng, Bihua Wu, Hai Li, Shune Xiao, Feng Lu, Zairong Wei, Chengliang Deng
Summary: This study found that stromal vascular fraction gel (SVF-gel) can promote the early healing of chronic wounds by secreting various cytokines and promoting cell proliferation and migration ability. Autologous SVF-gel transplantation in 20 patients with chronic wounds resulted in 100% wound closure eventually, with an average healing time of 28.3 days. Long-term follow-up ranging from 2 to 6 years showed no wound recurrence.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Orthopedics
Naomasa Yokota, Stephen Lyman, Hiroto Hanai, Kazunori Shimomura, Wataru Ando, Norimasa Nakamura
Summary: This study is the first head-to-head comparison of adipose-derived stromal cells (ASCs) and stromal vascular fraction (SVF) for the treatment of knee osteoarthritis (OA). The results showed that both ASCs and SVF injections significantly improved knee pain and function. ASC injections demonstrated better improvements in pain visual analog scale (VAS) and Knee injury and Osteoarthritis Outcome Score 5 (KOOS5) at 12 months. The use of ASCs over SVF may be warranted in the treatment of knee OA.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2022)
Correction
Cell & Tissue Engineering
Alisa Morss Clyne, Michele Marcolongo, Eric M. Darling, Nadeen O. Chahine
CELLULAR AND MOLECULAR BIOENGINEERING
(2019)
Article
Biophysics
Manisha K. Shah, Elizabeth A. Leary, Eric M. Darling
JOURNAL OF BIOMECHANICS
(2019)
Article
Engineering, Biomedical
Jessica S. Sadick, Lorin A. Crawford, Harry C. Cramer, Christian Franck, Shane A. Liddelow, Eric M. Darling
ANNALS OF BIOMEDICAL ENGINEERING
(2020)
Article
Biochemical Research Methods
R. Dubay, J. Fiering, E. M. Darling
Article
Biochemical Research Methods
Sylvia A. Sarnik, Bryan A. Sutermaster, Eric M. Darling
Summary: Mass-added density centrifugation (MADC) is a novel cell sorting method that addresses limitations of traditional cell sorting approaches, allowing rapid and efficient nonbinary separation of large quantities of cells based on surface protein levels. This technique is particularly useful for obtaining sufficient cell numbers for protein analysis from large samples, and can facilitate live cell enrichment for therapeutic applications.
Article
Engineering, Biomedical
Robert A. Gutierrez, Wenqiang Fang, Haneesh Kesari, Eric M. Darling
Summary: This study presents a versatile approach to measure cellular forces during mesenchymal condensation using hyper-compliant microparticles, revealing a wide range of tensile and compressive forces exerted by cells during tissue formation. Results show a period of relative equilibrium following an initial phase of diverse forces. Applying collagen coating enhances the forces exerted by cells, and the sensitivity of the force sensors allows for the detection of small changes in the microenvironment even at the earliest stages of development and morphogenesis.
Article
Chemistry, Multidisciplinary
Ryan Dubay, Joseph N. Urban, Eric M. Darling
Summary: Encapsulation of cells within hydrogel droplets has advanced significantly, enabling single-cell research and presenting a wide range of potential applications.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Cell & Tissue Engineering
Megan E. Dempsey, Olivia Woodford-Berry, Eric M. Darling
Summary: Researchers have developed a method to characterize antibody removal timelines for any cell-antibody combination, allowing for the study of broad mechanisms of antibody removal. The specific antibody tested determined the removal mechanism, while the cell type influenced the removal timelines. This method is relevant for quantifying an initial timeframe for uninhibited use of antibody-labeled cells.
CELLULAR AND MOLECULAR BIOENGINEERING
(2021)
Article
Oncology
Adrienne M. Parsons, Eric M. Darling
Summary: The study revealed the impact of time on adipose-derived stem/stromal cell (ASC) response to an inflammatory environment. Time played a role in the balance of pro- and anti-inflammatory ASC phenotypes, with IL-6 consistently present and IDO1 heterogeneously upregulated in later stages of sustained cytokine stimulation. Immune response of ASC remained constant for 96-168 hours after a pulse stimulus, and the presence of ASCs altered macrophage phenotype independent of ASC cytokine exposure time.
EXPERIMENTAL CELL RESEARCH
(2021)
Correction
Engineering, Biomedical
Robert A. Gutierrez, Wenqiang Fang, Haneesh Kesari, Eric M. Darling
Article
Biochemistry & Molecular Biology
Megan E. Dempsey, Graylen R. Chickering, Rafael D. Gonzalez-Cruz, Vera C. Fonseca, Eric M. Darling
Summary: This study demonstrates a workflow for surface protein discovery associated with cellular mechanophenotype. The method allows for the sorting and enrichment of live cells based on their mechanophenotype using surface protein biomarkers, followed by further characterization and differentiation studies.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell & Tissue Engineering
Robert A. Gutierrez, Vera C. Fonseca, Eric M. Darling
Summary: In this study, a microscale agarose microwell culture system was used to assess the chondrogenic response of adipose-derived stem cells (ASCs). The results demonstrated that high-throughput spheroid formats consistently induced chondrogenic differentiation, especially when seeding at a cell density of 5000 cells per spheroid. This opens up possibilities for studying tissue repair and remodeling.
CELLULAR AND MOLECULAR BIOENGINEERING
(2022)
Article
Environmental Sciences
Patritsia M. Stathatou, Christos E. Athanasiou, Marios Tsezos, John W. Goss, L. Camron Blackburn, Filippos Tourlomousis, Andreas Mershin, Brian W. Sheldon, Nitin P. Padture, Eric M. Darling, Huajian Gao, Neil Gershenfeld
Summary: Traces of heavy metals, such as lead, in water resources pose a global threat. Conventional treatment processes are ineffective in removing low concentrations of toxic heavy metals. However, using Saccharomyces cerevisiae, it is possible to efficiently remove trace lead from water through biosorption.
COMMUNICATIONS EARTH & ENVIRONMENT
(2022)
Article
Materials Science, Biomaterials
Kristin C. Meyer, Nicholas R. Labriola, Eric M. Darling, Bryan Kaehr
ADVANCED BIOSYSTEMS
(2019)