期刊
CELLULAR & MOLECULAR IMMUNOLOGY
卷 6, 期 2, 页码 105-110出版社
CHIN SOCIETY IMMUNOLOGY
DOI: 10.1038/cmi.2009.14
关键词
TCR; CDR3; CMV; pp65 peptide; CTL
类别
资金
- National Natural Science Foundation of China [30771952, 30771971]
- New Century Excellent Talents in University [NCET-07-0410]
- Natural Science Foundation of Guangdong Province [07117783]
- Major State Basic Research Development Program [2007CB512405]
Many viral epitope specific T cell receptors (TCRs) in MHC-matched individuals have been demonstrated to involve conserved amino acid motifs in beta chain complementarity-determining region 3 (CDR3). However, it is not sure whether the conserved motifs can also be found in TCR alpha chain. In previous studies, we developed a modified method to enlarge the percentage of cytomegalovirus (CMV) pp65 peptide-specific CD8(+) T cells in PBMC by continuous peptide stimulation in vitro, which provides sufficient number of specific T cells for detection. In this study, we further analyzed the restrictive usage of TCR V alpha and V beta gene families and investigated the CDR3 gene sequence of pp65 peptide-specific CD8(+) T cells. Analysis of CDR3 spectratypes suggested a restricted usage of TCR alpha chain AV8, AV12, AV21, AV31 families and TCR beta chain BV3, BV14, BV21, BV23, BV11 families in donor CD8(+) T cells stimulated by pp65 peptide. The sequences of these T cells involved similar sequence (TX) G(X)A in CDR3 region of TCR alpha chain and L(XT) G(X)A in TCR beta chain. Cellular & Molecular Immunology. 2009;6(2):105-110.
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